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1
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Characterization of wax-ester hydrolase from roots of white mustard (Sinapis alba L.) seedlings

100%
Kalinowska M., Wojciechowski Z. A.
|
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vol. 32
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issue 3
259-269
2
Content available remote

Characterization of acyllipid:sterol glucoside acyltransferase from oat (Avena Sativa L.) seedlings

81%
Misiak M., Kalinowska M., Wojciechowski Z. A.
Acta Biochimica Polonica
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1991
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vol. 38
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issue 1
43-45
3
Content available remote

UDP-glucose:solasodine glucosyltranslerase from eggplant (Solanum melongena L.) leaves: Partial purification and characterization

81%
Pączkowski C., Kalinowska M., Wojciechowski Z. A.
Acta Biochimica Polonica
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1997
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vol. 44
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issue 1
43-53
4
Content available remote

Evidence for essential histidine and dicarboxylic amino-acid residues in the active site of UDP-glucose : solasodine glucosyltransferase from eggplant leaves.

81%
Nawłoka P., Kalinowska M., Pączkowski C., Wojciechowski Z. A.
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2003
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vol. 50
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issue 2
567-572
EN
Effects of several chemical probes selectively modifying various amino-acid residues on the activity of UDP-glucose : solasodine glucosyltransferase from eggplant leaves was studied. It was shown that diethylpyrocarbonate (DEPC), a specific modifier of histidine residues, was strongly inhibitory. However, in the presence of excessive amounts of the enzyme substrates, i.e. either UDP-glucose or solasodine, the inhibitory effect of DEPC was much weaker indicating that histidine (or histidines) are present in the active site of the enzyme. Our results suggest also that unmodified residues of glutamic (or aspartic) acid, lysine, cysteine, tyrosine and tryptophan are necessary for full activity of the enzyme. Reagents modifying serine and arginine residues have no effect on the enzyme activity.
5
Content available remote

Hip and Knee Kinematics in Sagittal Plane during Stair Ascent and Descent in Children, Adolescents and Young Adults

81%
Syczewska M., Szczerbik E., Kalinowska M.
Journal of Human Kinetics
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2010
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vol. 26
45-49
EN
Ascending and descending stairs is a frequent activity in every-day living. Available data usually describe the ascent and descent pattern of young healthy adults, or elderly people.In order to assess the problems of peadiatric patients during stair ascent and descent, and to evaluate how treatment changes this pattern, a normative data base is needed. This data is lacking in the literature. Therefore, the aim of this study was to collect the kinematic data of healthy children, adolescents and young adults during stair locomotion and to assess the changes in range of movement in hip and knee joints in the sagittal plane. Twenty-seven healthy subjects, aged 6 to 21 years old, participated in the study. The subjects were climbing stairs with dimensions comparable to steps in public building.The data were collected using optoelectronic motion system VICON 460. Helen Hayes marker set and Plug-In-Gait model were used. The data were further processed using Polygon and Matlab softwares. The subjects were divided into 6 subgroups, according to their body stature.Hip and knee kinematics in sagittal plane during stair ascent and descent are reported for all 6 subgroups.The main factor determining the dynamic range of movement in the hip and knee joints during stair locomotion is the body stature / step height ratio. The present paper presents detailed data, which could be used as reference data for assessment of stair locomotion of paediatric patients.
6
Content available remote

Appearance of poly(A)-rich RNA in germinating pea seeds

71%
Sieliwanowicz B., Kalinowska M., Chmielewska I.
Acta Biochimica Polonica
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1977
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vol. 24
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issue 1
59-64
7
Content available remote

Use of instrumented spasticity tests in clinical applications. Preliminary results

62%
Szczerbik E., Kalinowska M., Graff K., Olczak-Kowalczyk D., Dąbrowska-Gontarczyk A., Jelonek E., Syczewska M.
Biomedical Human Kinetics
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2015
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vol. 7
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issue 1
EN
Study aim: the aim of the study was to evaluate whether the use of instrumented versions of spasticity tests would provide us with clinically more useful information on the patient’s status. Material and methods: the study included 19 children, 8–17 years old: 7 girls and 12 boys. Pendulum, velocity, and popliteal tests were performed using the Vicon system (knee joint angle, 8 muscles EMG). Dynamic movement ranges of the knee joint during velocity and popliteal tests (ROM), indices of the pendulum test, and muscle activity in dependence of velocity of movement (MA, MAST) were calculated. Correlation coefficients between ROM, Vmax, MA, and MAST were calculated to show whether instrumentation of clinical tests can validate the patient’s status more precisely. Results: Vmax value from the pendulum test does not always correlate with ROM. Scores of MA and MAST do not correlate with ROM. Vmax generally does not correlate with MA or MAST. Conclusions: ROM is one of the most important parameters reflecting the level of spasticity but it is not sensitive enough to detect small changes in the patient’s status. In that case, Vmax of the pendulum test and the number of activated muscles in velocity and popliteal tests could become important tools to assess changes in spasticity level, especially when motion systems are more commonly available.
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