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1

Lipopolysaccharides of the genus Proteus

100%
Sidorczyk Z.
Postępy Higieny i Medycyny Doświadczalnej
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1996
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vol. 50
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issue 5
401-417
EN
In this review up-to-date knowledge on chemical structure and epitope specificity of Proteus lipopolysaccharides is summarized, with special emphasis to P. penneri LPS
2

Application of two different kinds of sera against the Proteus penneri lipopolysaccharide core region in search of epitopes determining cross-reactions with antibodies

51%
Palusiak A., Dzieciatkowska M., Sidorczyk Z.
Archivum Immunologiae et Therapiae Experimentalis
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2008
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vol. 56
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issue 2
135-140
EN
Introduction: Proteus penneri lipopolysaccharide (LPS) core regions are characterized by a greater structural variability than that observed in other Enterobacteriaceae. This fact and the small amount of published data concerning the serological activity of this part of P. penneri LPS prompted an examination of which fragment might determine cross-reactions with antibodies. To date, such epitopes have been found in the LPS core regions of P. mirabilis and P. vulgaris strains. Materials and Methods: Proteus sp. LPSs were tested with unabsorbed rabbit antisera by enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot, and once again by ELISA or passive immunohemolysis after the absorption of these antisera with selected LPSs. Results: The serological studies of P. penneri 8 LPS demonstrated antibodies in the tested antisera recognizing a common epitope located in the core regions of six of the LPSs, i.e. P. penneri 8, 34, 133, 7, 14, and 15. Additionally, another type of antibody directed against some fragment of P. penneri 13 and the core regions of other LPSs investigated was observed in one antiserum. Conclusions: A distal, trisaccharide fragment of the P. penneri 8 LPS core region is suggested to determine the cross-reactions of the tested antisera with the six P. penneri LPSs.
3

Characterization and serological classification of a collection of Proteus penneri clinical strains

51%
Drzewiecka D., Zych K., Sidorczyk Z.
Archivum Immunologiae et Therapiae Experimentalis
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2004
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vol. 52
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issue 2
121-128
EN
Chronic Granulomatous disease bacteria of the genus Proteus, which are a common cause of urinary tract infections, are divided into four species: P. mirabilis, P. vulgaris, P. penneri, and P. hauseri, and three unnamed genomospecies, Proteus 4, 5, and 6 (single-strain species P. myxofaciens was isolated from the gypsy moth). Establishing the serological classification of these species would aid in completing the classification scheme of the whole genus Proteus and in applying serological methods in diagnostic procedures and epidemiological investigations for these opportunistic pathogens. The aim of this research was a serological characterization and classification of 57 Proteus penneri clinical strains, isolated from patients from different countries all over the world, into Proteus O serogroups. Purified lipopolysaccharides (LPSs) extracted from 57 P. penneri strains were used as antiandgens in enzyme immunosorbent assay (EIA), SDS/PAGE, and Western blot techniques, and alkali-treated LPSs in passive immunohemolysis test (PIH), inhibition of PIH, and absorption of rabbit polyclonal O-antisera. Results: That result confirms the serological distinction of this species within the genus Proteus, and may have diagnostic significance. Conclusions: As a result of serological studies of LPSs extracted from the P. penneri strains, one new Proteus serogroup, represented by the P. penneri 97 strain, was established. Three further strains were classified into the Proteus serogroup O8, which had not contained any P. penneri strains before. All the remaining strains were classified into 11 already existing Proteus O serogroups. It is important to emphasize that 72% of studied strains were classified into serogroups that contain P. penneri strains only.
4

Serological classification and epitope specificity of Proteus penneri S29 lipopolysaccharide

51%
Zych K., Siwinska M., Sidorczyk Z.
Archivum Immunologiae et Therapiae Experimentalis
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2005
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vol. 53
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issue 6
540-545
EN
Gram-negative bacteria of genus Proteus are common human intestinal and urinary tract pathogens. In the genus Proteus there are four clinically important named species: P. mirabilis, P. vulgaris, P. penneri, and P. hauseri, and three unnamed Proteus genomospecies: 4, 5, and 6. The clinical significance of P. penneri, described in 1982 as a new species, is poorly documented. The aim of this work is serological characterization and classification of a ceftriaxone-susceptible P. penneri S29 strain isolated from a 34-year-old patient with postneurosurgical meningitis. In this characterization we will also include a ceftriaxon-resistant strain, P. penneri R15, isolated from the same patient after 12 days' treatment with ceftriaxon and other antibiotics. Rabbit polyclonal O-antisera were obtained against these two strains and purified lipopolysaccharides (LPS) were extracted from the bacterial mass of the P. penneri S29 and R15 strains. In the serological investigations the following tests were used: enzyme immunosorbent assay (EIA), passive immunohemolysis (PIH), inhibition of these tests, absorption of rabbit O-antisera with the respective LPS, and repeated PIH, SDS/PAGE, and Western blot techniques. The serological studies of the LPS extracted from both P. penneri strains showed the identity of both preparations of O-polysaccharides from LPS.. In P. penneri S29 O-antiserum, four different types of antibodies were described and characterized. Both investigated P. penneri S29 and R15 strains were classified to the Proteus O31ab serogroup.
5

Characterization and serological classification of O-specific polysaccharide of Proteus mirabilis TG 276-90 from Proteus serogroup O34

45%
Kolodziejska K., Siwinska M., Zych K., Rozalski A., Sidorczyk Z.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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vol. 54
|
issue 3
223-226
EN
Introduction: Gram-negative bacteria of the genus Proteus from the family Enterobacteriaceae are currently divided into the five species P. mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens and three unnamed Proteus genomospecies 4, 5, and 6. They are important facultative human and animal pathogens which, under favorable conditions, cause mainly intestinal and urinary tract infections, sometimes leading to serious complications such as acute or chronic pyelonephritis and the formation of bladder and kidney stones. In this study we report on the serological properties of the lipopolysaccharide (LPS) of Proteus mirabilis TG 276-90, whose O-polysaccharide chemical structure was described earlier. Materials and Methods: LPS and alkali-treated LPS of a few serologically related Proteus strains and O-antisera against P. mirabilis TG 276-90 and CCUG 4669 (O34) were used. Serological characterization of P. mirabilis TG 276-90 O-specific polysaccharide was done using enzyme immunosorbent assay, passive immunohemolysis test (PIH), inhibition of these tests, SDS/PAGE and Western blot techniques, absorption of rabbit polyclonal O-antisera, and repeated PIH test. Results: Structural and serological investigations showed that the O-polysaccharides of P. mirabilis TG 276-90 and P. vulgaris O34 are identical and that their LPSs differ only in epitopes in the core part. Therefore these two strains could be classified into the same Proteus O34 serogroup. Conclusions: The serological data showed that the beta-D-GalpNAc-(14)-alpha-D-GalpNAc disaccharide is an important epitope of the P. mirabilis TG 276-90 and P. vulgaris O34 LPSs, shared by the P. mirabilis O16 and P. vulgaris TG 251 LPSs. It is responsible for cross-reactions with P. mirabilis TG 276-90 and P. vulgaris O34 O-antisera.
6

Classification of Proteus mirabilis TG 115 and CCUG 10701 into the Proteus O23 serogroup based on chemical and serological studies of O-polysaccharides

39%
Zablotni A., Perepelov A. V., Kolodziejska K., Zych K., Knirel Y. A., Sidorczyk Z.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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vol. 54
|
issue 6
411-417
EN
Introduction: Bacteria of the genus Proteus are a common cause of urinary tract infections. The O-polysaccharide (OPS) chain of their lipopolysaccharide (LPS) defines the serological specificity of strains. Based on the OPS structures and the immunospecificity of the LPS, Proteus strains have been classified into 74 O-serogroups. Materials and Methods: The OPS of P. mirabilis TG 115 was obtained by mild acid degradation of the LPS and studied by 1H and 13C nuclear magnetic resonance spectroscopy. Antisera were raised by immunization of rabbits with heat-killed bacteria. Serological studies were performed using enzyme immunosorbent assay, passive immunohemolysis, inhibition experiments, absorption of O-antisera, and Western blot.Results: The following structure of the P. mirabilis TG 115 OPS was established: 2)--D-GalpA-(13)--D-GalpNAc-(14)--D-GalpA-(13)--D-GlcpNAc-(1 The same structure has been reported previously for the O-polysaccharides of P. mirabilis CCUG 10701 (O74) and P. mirabilis 41/57 (O23), except that they contain O-acetyl groups in non-stoichiometric quantities. Serological studies showed the antigenic identity of the three strains and their close serological relatedness to P. vulgaris 44/57. Conclusions: Based on the OPS structures and serological data, it is suggested to classify P. mirabilis 41/57, TG 115, and CCUG 10701 into one subgroup and P. mirabilis 42/57 and P. vulgaris 43/57 and 44/57 into another subgroup of the Proteus O23 serogroup.
7

Serological classification and epitope specificity of Proteus vulgaris TG 251 from Proteus serogroup O65

39%
Zych K., Kolodziejska K., Drzewiecka D., Perepelov A. V., Knirel Y. A., Sidorczyk Z.
|
2007
|
vol. 55
|
issue 3
187-191
EN
Introduction: Proteus rods are currently subdivided into five named species, i.e. Proteus mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens, and three unnamed Proteus genomospecies 4 to 6. Based on the serospecificity of the lipopolysaccharide (LPS; O-antigen), strains of P. mirabilis and P. vulgaris were divided into 49 O-serogroups and 11 additional O-serogroups were proposed later. About 15 further O-serogroups have been proposed for the third medically important species, P. penneri. Here the serological classification of P. vulgaris strain TG 251, which does not belong to these serogroups, is reported. Serological investigations also allowed characterization of the epitope specificity of its LPS. Materials and Methods: Purified LPSs from five Proteus strains were used as antigens in enzyme immunosorbent assay (EIA), SDS/PAGE, and Western blot and alkali-treated LPSs in the passive immunohemolysis (PIH) test, inhibition of PIH and EIA, and absorption of the rabbit polyclonal O-antisera with the respective LPS. Results: The serological studies of P. vulgaris TG 251 LPS indicated the identity of its O-polysaccharide with that of P. penneri O65. The antibody specificities of P. vulgaris TG 251 and P. penneri O65 O-antisera, were described. Conclusions: P. vulgaris TG 251 was classified to the Proteus O65 serogroup. Two disaccharide-associated epitopes present in P. vulgaris TG 251 and P. penneri O65 LPSs are suggested to be responsible for cross-reactions with three heterologous Proteus strains.
8

Structural and serological characterization of the lipopolysaccharide from proteus penneri 20 and classification of cross-reacting proteus penneri strains 10, 16, 18, 20, 32 and 45 in proteus serogroup O17

33%
Sidorczyk Z., Toukach F. V., Zych K., Arbatsky N. P., Drzewiecka D., Ziolkowski A., Shashkov A. S., Knirel Y. A.
Archivum Immunologiae et Therapiae Experimentalis
|
2002
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vol. 50
|
issue 5
345-355
EN
O-specific polysaccharide (O-antigen) of the lipopolysaccharide of Proteus penneri 20 was studied using sugar analysis along with various one- and two-dimensional NMR spectroscopy techniques. The structure of the polysaccharide was established. It has the same carbohydrate backbone structure as that described earlier for P. penneri 16, in which the positions of the O-acetyl groups have not been determined. P. penneri 20 O-antiserum showed a strong cross-reactivity with the lipopolysaccharides of P. penneri 10, 16, 18, 32, 45 and P. mirabilis O17. These data enable classifying these strains together with P. penneri 20 in one Proteus serogroup, O17.
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