Search results

1

GMO w perspektywie ewolucji

100%

Bartnik E.

Kosmos

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2007

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vol. 56

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issue 3-4

217-219

EN

Genetically modified organisms are perceived by many as unnatural man-made creations, which are an inappropriate intervention into the natural order. In this paper I try to show that had genes not jumped and recombined, we would not be here.

2

Ludzki genom mitochondrialny

100%

Bartnik E.

Kosmos

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2009

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vol. 58

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issue 3-4

555-558

EN

The human mitochondrial genome is a small 16.5 kb circular double-stranded DNA molecule containing 37 genes. Mutations in this DNA can lead to various diseases, and the mitochondrial DNA (mtDNA) genome which is maternally inherited has been very often used in studies on human evolution. Mitochondria of all humans appear to originate from one woman who lived in Africa about 180000 years ago. Various parts of the mtDNA may not evolve at the same rate, and the different mitochondrial DNA haplogroups may not be totally functionally equivalent, raising questions as to the involvement of mitochondria in various human diseases and the process of aging.

3

Contemporary techniques of data acquisition for preparation of numerical models of hydrotechnical structures

45%

Popielski P., Zaczek-Peplinska J., Bartnik E., Kasprzak A., Smoliński B.

Czasopismo Techniczne

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-

PL

This paper presents an analysis of possible uses of contemporary data acquiring methods for elaborating hydrotechnical object numerical models. The subject is considered in two aspects – preparation of a geometrical model based on the results of a geodetic survey of the structure as well as subsoil parameters, and data acquisition for building material characteristics. The results presented for non-invasive measurements of hydrotechnical concrete parameters and geometry of the modelled object are based on the example of Rożnów Dam. The research uses data obtained by terrestrial laser scan and sclerometer tests (taken with a S chmidt hammer).

4

Nuclear and mitochondrial genome responses in HeLa cells treated with inhibitors of mitochondrial DNA expression

45%

Piechota J., Szczęsny R., Wolanin K., Chlebowski A., Bartnik E.

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2006

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vol. 53

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issue 3

485-495

EN

The influence of mutations in the mitochondrial DNA (mtDNA) on the bioenergetic metabolism of the cell is still poorly understood. Many of the mutations in the mtDNA affect the expression of the mitochondrial genome. Investigations on cells from patients are not easy, especially as the mitochondrial DNA is heteroplasmic and this state is changed in culture. Moreover, the nuclear background and the mitochondrial haplotype may affect the behaviour of cells. Transfer of patient mitochondria to rho zero cell lines is also not optimal as these cells in general have many nuclear changes which may also affect cell behaviour. Thus, we decided to use inhibitors of mitochondrial genome expression, such as thiamphenicol, ethidium bromide and dideoxycytidine to investigate the bioenergetic metabolism of HeLa cells. We found that oxidative phosphorylation and glycolysis participate equally in ATP production in HeLa cells and that decreased activity of the respiratory chain leads to increased glycolysis and the reduction of cell growth. Insufficient ATP production in the oxidative phosphorylation process was not compensated by increased proliferation of the mitochondria. However, we were able to show that there are some mechanisms compensating limited expression of the mitochondrial genome within the mitochondria. Experiments with dideoxycytidine revealed that 10-fold decrease of the mtDNA copy number resulted in almost normal activity of cytochrome c oxidase. We found that mtDNA depletion is compensated mostly on the level of RNA metabolism in the mitochondria. Thus, our results are in agreement with the hypothesis that transcription initiation rather than mtDNA copy number is a rate limiting factor for expression of the mitochondrial genome.

5

Genetyka - od Mendla do genomiki.

44%

Bartnik E.

Kosmos

|

2000

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vol. 49

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issue 3

395-398

6

Comparison between the Polish population and European populations on the basis of mitochondrial morphs and haplogroups.

39%

Piechota J., Tońska K., Nowak M., Kabzińska D., Lorenc A., Bartnik E.

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vol. 51

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issue 4

883-895

EN

Polymorphisms in mitochondrial DNA (mtDNA) were analyzed in 152 samples from the Polish population using restriction enzymes AvaI, BamHI, HaeII, HpaI and PstI. Additionally, each sample was classified into the appropriate haplogroup. When required, appropriate fragments were sequenced to establish the exact polymorphic sites. We found one new morph for PstI and six new morphs for AvaII. Some detected morphs have previously been described as population specific morphs in different regions of the world. All polymorphisms were classified into 31 different haplotypes. 21 of them were detected in single individuals. The Polish population was compared with other populations from different regions. Moreover, we have obtained evidence for mutation hot spots in the mtDNA coding region. Our results indicate that AvaII morph and haplogroup composition of the Polish population is similar to other European populations and has a distribution typical for this part of the world. However, statistically significant differences in haplogroup composition were found between the Polish population and Italian and Finnish populations.

7

Heteroplasmy analysis in the Polish patients with 11778A mutation responsible for Leber hereditary optic neuropathy.

39%

Mroczek-Tońska K., Ratajska D., Guillot C., Sąsiek M., Ambroziak A., Lubos L., Bartnik E.

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2002

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vol. 49

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issue 1

257-262

EN

We have analysed the heteroplasmy level in 11 individuals from 3 families harbouring the mitochondrial 11778A mutation responsible for Leber hereditary optic neuropathy using last cycle hot PCR. The mutation level exceeded 90% both in affected and in unaffected individuals. We also checked whether any of the families belonged to the J haplogroup of mitochondrial DNA and obtained a negative result.

8

Yeast nuclear PET127 gene can suppress deletions of the SUV3 or DSS1 genes: An indication of a functional interaction between 3' and 5' ends of mitochondrial mRNAs

39%

Węgierski T., Dmochowska A., Jabłonowska A., Dziembowski A., Bartnik E., Stępień P. P.

Acta Biochimica Polonica

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1998

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vol. 45

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issue 4

935-940

9

Evolutionary conservation of the transcribed spacer sequences of the rDNA repeat unit in three species of the genus Aspergillus

39%

Borsuk P., Gniadkowski M., Kucharski R., Bisko M., Kanabus M., Stępień P. P., Bartnik E.

Acta Biochimica Polonica

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1994

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vol. 41

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issue 1

73-77

10

Mitochondrial DNA in pediatric leukemia patients

39%

Kodroń A., Ghanim M., Krawczyk K., Stelmaszczyk-Emmel A., Tońska K., Demkow U., Bartnik E.

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2017

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vol. 64

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issue 1

183-187

EN

Numerous studies of mitochondrial DNA (mtDNA) in cancer have shown differences between mtDNA sequences in tumor and normal tissue and at various stages of cancer treatment in the same patient. However, there is little data on acute lymphoblastic leukemia (ALL), the most common type of leukemia in children. In this study we compared mitochondrial sequence variation in the D-loop region and in 5 genes of mtDNA in bone marrow samples of 6 pediatric patients with ALL at various stages of therapy. We found several common polymorphisms and one variant at position 3688 whose level varied during leukemia treatment. Our results suggest that mitochondrial DNA mutations, whose levels change during patient treatment, could be potential biomarkers for monitoring treatment efficacy and disease progression.

11

How many 5S rRNA genes and pseudogenes are there in Aspergillus nidulans?

39%

Pelczar P., Fiett J., Bartnik E.

Acta Biochimica Polonica

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1994

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vol. 41

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issue 4

449-452

12

Differential stability of mitochondrial mRNA in HeLa cells

33%

Piechota J., Tomecki R., Gewartowski K., Szczęsny R., Dmochowska A., Kudła M., Dybczyńska L., Stepien P. P., Bartnik E.

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2006

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vol. 53

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issue 1

157-168

EN

The physiological significance and metabolism of oligoadenylated and polyadenylated human mitochondrial mRNAs are not known to date. After study of eight mitochondrial transcripts (ND1, ND2, ND3, ND5, CO1, CO2, ATP6/8 and Cyt. b) we found a direct correlation between the half-lives of mitochondrial mRNAs and their steady-state levels. Investigation of the mt-mRNA decay after thiamphenicol treatment indicated that three transcripts (ND2, ND3 and Cyt. b) are significantly stabilized after inhibition of mitochondrial translation. Careful analysis one of them, ND3, showed that inaccurate processing of the H-strand RNA precursor may occasionally occur between the ND3 and tRNAArg locus leading to synthesis of ND3 mRNAs lacking the STOP codon. However, analysis of the oligo(A) fraction observed in case of the ND3 indicates that partially polyadenylated mRNAs are linked rather to the transcription process than to the translation-dependent deadenylation. Analysis of ND3 mRNA turnover in cells with siRNA-mediated knock-down of the mitochondrial poly(A) polymerase shows that strongly decreased polyadenylation does not markedly affect the decay of this transcript. We present a model where oligoadenylated mitochondrial transcripts are precursors of molecules containing full length poly(A) tails.

13

A human putative Suv3-like RNA helicase is conserved between Rhodobacter and all eukaryotes

33%

Dmochowska A., Kalita K., Krawczyk M., Golik P., Mroczek K., Lazowska J., Stępień P. P., Bartnik E.

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Refine search results

GMO w perspektywie ewolucji

Ludzki genom mitochondrialny

Contemporary techniques of data acquisition for preparation of numerical models of hydrotechnical structures

Nuclear and mitochondrial genome responses in HeLa cells treated with inhibitors of mitochondrial DNA expression

Genetyka - od Mendla do genomiki.

Comparison between the Polish population and European populations on the basis of mitochondrial morphs and haplogroups.

Heteroplasmy analysis in the Polish patients with 11778A mutation responsible for Leber hereditary optic neuropathy.

Yeast nuclear PET127 gene can suppress deletions of the SUV3 or DSS1 genes: An indication of a functional interaction between 3' and 5' ends of mitochondrial mRNAs

Evolutionary conservation of the transcribed spacer sequences of the rDNA repeat unit in three species of the genus Aspergillus

Mitochondrial DNA in pediatric leukemia patients

How many 5S rRNA genes and pseudogenes are there in Aspergillus nidulans?

Differential stability of mitochondrial mRNA in HeLa cells

A human putative Suv3-like RNA helicase is conserved between Rhodobacter and all eukaryotes