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1
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Content available

Controlling partnering relations in construction operations using fuzzy reasoning

100%
Radziszewska-Zielina E., Szewczyk B., Radziszewska-Zielina E., Szewczyk B.
Archives of Civil Engineering
|
2015
|
issue 3
2

Enzyme-linked immunosorbent assay (ELISA) as a method for detection of specific antibodies in animal sera

89%
Gut-Winiarska M., Szewczyk B.
Biotechnologia
|
2001
|
issue 3
53-70
EN
This paper aims to evaluate different forms of the enzyme-linked immunosorbent assays (ELISA) for the detection of virus-specific antibodies and focuses on factors which may influence the diagnostic reliability of such tests. The differences between competitive and non-competitive ELISAs are described, with reference to the influence of the antigen, the conjugated antibody, and the test sample on the test results. Attention is drawn to interference, which may result in false positive or false negative test results. The use of monoclonal antibodies and discriminatory tests is briefly discussed. Diagnostic reliability is described for tests that are used in monitoring or eradication programmes, emphasising the consequences of false negative or false positive results.
3

Application of transfer onto PVDF and nitrocellulose membranes in the purification and structural analyses of proteins

89%
Szewczyk B., Bienkowska-Szewczyk K.
Biotechnologia
|
1996
|
issue 1
129-150
EN
Replicas of SDS-PAGE gels widen the range of application of electrophoresis to the studies of diverse biological properties of proteins as well as to the elucidation of their structures. Most often proteins are transferred to membranes made of polyvinylidene difluoride (PVDF) or nitrocelulose.Usually, a "semi-dry" blotting apparatus is used for electrophoretic transfer.SDS-PAGE gel replicas are used for the studies of these biological properties which cannot be studied directly on polyacrylamide gels. The major applications of this technique is in the immunoblotting which allows for the antigen-antibody reaction on the membrane.This reaction, after necessary amplification, is visualized with a variety of reagents.Protein microsequencing is another important application of SDS-PAGE gel replicas.In this case, a replica is stained with a general protein stain, and a band of interest is excised and used to obtain partial or complete amino acid sequence in an automatic sequencer.
4
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Market research concerning functional and material solutions employed in nursery facilities

76%
Radziszewska-Zielina E., Grabowska W., Szewczyk B., Radziszewska-Zielina E., Grabowska W., Szewczyk B.
Archives of Civil Engineering
|
2019
|
vol. 65
|
issue 1
5

Baculovirus expression of genes encoding the glycoproteins of animal herperviruses

72%
Bienkowska-Szewczyk K., Kochan G., Tyborowska J., Szewczyk B.
Postępy Higieny i Medycyny Doświadczalnej
|
1996
|
vol. 50
|
issue 5
545-548
EN
Baculovirus system has been used for the expression of genes of herpesvirus glycoproteins. In this system highly glycosylated proteins are obtained. The glycoproteins may find potential use as vaccines and in diagnosis of pseudorabies.
6

Elution of glycoproteins blotted from polyacrylamide gel

72%
Pilat Z., Ficinska J., Bienkowska-Szewczyk K., Szewczyk B.
Postępy Higieny i Medycyny Doświadczalnej
|
1996
|
vol. 50
|
issue 5
541-544
EN
The paper describes the procedure of efficient elution of glycoproteins from Immobilon membrane after their blotting from polyacrylamide gel. The yield of elution from membrane is 30-70%.
7
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Managing information flow in self-organising networks of communication between construction project participants

64%
Radziszewska-Zielina E., Śladowski G., Kania E., Sroka B., Szewczyk B., Radziszewska-Zielina E., Śladowski G., Kania E., Sroka B., Szewczyk B.
Archives of Civil Engineering
|
2019
|
vol. 65
|
issue 2
8
Publication available in full text mode
Content available

Development of a recombinant NP protein based indirect ELISA for the detection of antibodies against Newcastle disease virus and differentiation of infected or recombinant vaccine immunized chickens

52%
Domańska-Blicharz K., Tyborowska J., Sajewicz-Krukowska J., Olszewska-Tomczyk M., Rąbalski Ł., Kucharczyk K., Szewczyk B., Śmietanka K., Domańska-Blicharz K., Tyborowska J., Sajewicz-Krukowska J., Olszewska-Tomczyk M., Rąbalski Ł., Kucharczyk K., Szewczyk B., Śmietanka K.
Polish Journal of Veterinary Sciences
|
2019
|
issue 3
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