Search results

Sort By:

Limit search:

A novel single-nucleotide polymorphism of the visfatin gene and its associations with performance traits in the chicken

100%

Han R.-L., Lan X.-Y., Zhang L.-Z., Ren G., Jing Y.-J., Li M.-J., Zhang B., Zhao M., Guo Y.-K., Kang X.-T., Chen H.

vol. 51

issue 1

59-65

Visfatin is a peptide that is predominantly expressed in visceral adipose tissue and is hypothesized to be related to obesity and insulin resistance. In this study, a novel silent single-nucleotide polymorphism (SNP) was found in exon 7 of the chicken visfatin gene (also known as PBEF1) by single-stranded conformation polymorphism (SSCP) and DNA sequencing. In total, 836 chickens forming an F2 resource population of Gushi chicken crossed with Anka broiler were genotyped by XbaI forced RFLP, and the associations of this polymorphism with chicken growth, carcass characteristics, and meat quality were analyzed. Significant associations were found between the polymorphism and 4-week body weight (BW4), 6-week body weight (BW6), 4-week body slanting length (BSL4), fat bandwidth (FBW), breast muscle water loss rate (BWLR) and breast muscle fiber density (BFD) (P < 0.05), as well as 4-week breastbone length (BBL4) (P < 0.01). These observations suggested that the polymorphism in exon7 of the visfatin gene had significant effects on the early growth traits of chicken.

Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits

100%

Huang Y.-Z., Zhang E.-P., Chen H., Wang J., Li Z.-J., Huai Y.-T., Ma L., Lan X.-Y., Ren G., Lei C.-Z., Fang X.-T., Wang J.-Q.

vol. 51

issue 2

199-202

The nucleophosmin 1 gene (NPM1) encodes a multifunctional nucleolar phosphoprotein that plays a crucial role in the control of various aspects of cell growth and homeostasis. In this study, the coding region of the NPM1 gene was screened in 1035 individuals of 4 Chinese cattle breeds by DNA sequencing and polyacrylamide gel electrophoresis. A novel 12-bp deletion mutation was identified in the coding region of the NPM1 gene. The PCR products of primer NPM1-P2 exhibited 3 genotypes and 2 alleles: 178 bp (denoted as W) and 166 bp (denoted as D). Genotype DD and allele D were predominant in the studied populations. Association analysis with growth traits in the Nanyang breed (N = 265) showed that the animals with genotype DD had significantly greater birth weight, body weight, body length, and heart girth than those with genotype WD (P < 0.01 or P < 0.05) at birth and after 6 months and 12 months, but not at 18 and 24 months of age. Results of this study suggest that the NPM1 gene is a candidate gene for growth traits in cattle.

Refine search results

2 Journal of Applied Genetics

2 2010

2 Chen H.

2 Lan X.-Y.

2 Ren G.

1 Fang X.-T.

1 Guo Y.-K.

1 Han R.-L.

1 Huai Y.-T.

1 Huang Y.-Z.

1 Jing Y.-J.

1 Kang X.-T.

1 Lei C.-Z.

1 Li M.-J.

1 Li Z.-J.

1 Ma L.

1 Wang J.

1 Wang J.-Q.

1 Zhang B.

1 Zhang E.-P.

1 Zhang L.-Z.

1 Zhao M.

Search results

A novel single-nucleotide polymorphism of the visfatin gene and its associations with performance traits in the chicken

Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits