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  1. 1 Postępy Higieny i Medycyny Doświadczalnej

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  1. 1 1997

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  1. 1 Grzybowska E.

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Analysis of aromatic DNA adducts in the inhabitants of upper Silesia

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Grzybowska E.
Postępy Higieny i Medycyny Doświadczalnej
|
1997
|
vol. 51
|
issue 2
185-203
EN
Aromatic DNA adducts levels were measured in white blood cells collected from three groups of volunteers: occupationally exposed group (cokery workers), environmentally exposed group (residents from Silesia), control group (inhabitants of Biala Podlaska) using ELISA and 32P-postlabelling methods. The highest level of DNA adducts was observed in the occupationally exposed group, slightly lower in the environmentally exposed group and much lower in the control group. Seasonal variations in the adducts levels were found in total white blood cells and in lymphocytes showing that the levels of DNA adducts reflect the present exposure to genotoxic agents in the air. It was also shown using 32P-postlabelling with HPLC that not only adducts with polycyclic aromatic hydrocarbons were found in human DNA.
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