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Sulphur XANES Analysis of Cultured Human Prostate Cancer Cells

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Kwiatek W., Podgórczyk M., Paluszkiewicz C., Balerna A., Kisiel A.

Acta Physica Polonica A

2008

vol. 114

issue 2

463-470

Prostate cancer is one of the most commonly diagnosed cancers in men throughout the world. It is believed that changes to the structure of protein binding sites, altering its metabolism, may play an important role in carcinogenesis. Sulphur, often present in binding sites, can influence such changes through its chemical speciation. Hence there is a need for precise investigation of coordination environment of sulphur. X-ray absorption near edge structure spectroscopy offers such possibility. Cell culture samples offer histologically well defined areas of good homogeneity, suitable for successful and reliable X-ray absorption near edge structure analysis. This paper presents sulphur speciation data collected from three different human prostate cancer cell lines (PC-3, LNCaP and DU-145). Sulphur X-ray absorption near edge structure analysis was performed on K-edge structure. The spectra of cells were compared with those of cancerous tissue and with organic substances as well as inorganic compounds.

Distinguishing Prostate Cancer from Hyperplasia

73%

Kwiatek W., Banaś A., Banaś K., Podgórczyk M., Dyduch G., Falkenberg G., Gajda M., Cichocki T.

Acta Physica Polonica A

2006

vol. 109

issue 3

377-381

The prostate gland is the most common site of neoplastic disorders in men. The knowledge of the pathogenesis of benign prostate hyperplasia and prostate cancer is still under investigation. X-ray fluorescence analysis using synchrotron radiation seems to be an appropriate technique to study the elemental composition of biological tissues. The measurements presented in this paper were carried out on the L-beam line at the HASYLAB, DESY (Germany). The observed concentrations of Fe, Cu, Mn, and Zn are significantly higher in cancerous tissues, as compared to normal and hyperplastic ones. Our results reveal also the existence of the two types of hyperplasia.

Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis

73%

Kwiatek W., Paluszkiewicz C., Banaś A., Kisiel A., Podgórczyk M., Marcelli A., Cestelli Guidi M., Piccinini M.

Acta Physica Polonica A

2009

vol. 115

issue 2

602-605

The infrared spectromicroscopy is a quite recent technique rapidly developing thanks to the availability of new instruments and new brilliant synchrotron radiation sources in different areas and in particular to biomedical researches. In order to achieve a diffraction limited spatial resolution in tissue samples, we performed experiments at SINBAD, the synchrotron infrared beamline of the Laboratori Nazionali di Frascati. We characterized the chemical composition of prostate tissue samples taken from patients affected by prostate cancer disease. Different sizes of the pinholes were considered for the measurements. In the case of prostate tissue sections the results show the possibility to determine the intensity ratio of the CH_2 and CH_3 bands set at 2930 cm^{-1} and 2960 cm^{-1}, respectively. Experiments were also performed with a pinhole of 5 μm of diameter and the differences in both histological and chemical compositions of such samples were determined.

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3 Acta Physica Polonica A

1 2009

1 2008

1 2006

3 Kwiatek W.

3 Podgórczyk M.

2 Banaś A.

2 Kisiel A.

2 Paluszkiewicz C.

1 Balerna A.

1 Banaś K.

1 Cestelli Guidi M.

1 Cichocki T.

1 Dyduch G.

1 Falkenberg G.

1 Gajda M.

1 Marcelli A.

1 Piccinini M.

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Sulphur XANES Analysis of Cultured Human Prostate Cancer Cells

Distinguishing Prostate Cancer from Hyperplasia

Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis