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1

Ultrastructural and cytochemical identification of peroxisomes in Balantidium coli, Ciliophora

100%
Skotarczak B.
Folia Biologica
|
1997
|
vol. 45
117-120
EN
Peroxisomes of the trophozoites of Balantidium coli isolated from pig intestine content were investigated, using ultrastructural and cytochemical techniques. The peroxisomes of B. coli trophozoites from pigs with subclinical balantidiasis are less then 0.8 mm in diameter whereas those from pigs with acute balantidiasis are greater than 0.8 mum in diameter. In all the trophozoites peroxisomes are round, oval or dumb-bell shaped. Catalase as an indicative enzyme was detected by cytochemical techniques in B. coli peroxisomes.
2

Cytochemical identification of mucocysts in Balantidium coli trophozoites

100%
Skotarczak B.
Folia Biologica
|
1999
|
vol. 47
|
issue 1-2
61-65
EN
The mucocyst ultrastructure in B. coli has not been described so far. As demonstrated in this work, cytoenzymatic assays on B. coli with the use of a reaction-detecting membrane-coupled hydrolase, i.e., ATP-ase, permitted identification of the mucocysts in the ciliate studied. The shape, size, and location of mucocysts in B. coli trophozoites were found to correspond to descriptions of these structures in other ciliates. The mucocysts were more numerous in B. coli trophozoites isolated from the symptomatic balantidiosis-affected pigs (Group I), and the product of reaction to ATP-ase was more copious than in Group II trophozoites. However, not all the bubble-like structures with similar morphological features reacted positively to the enzyme. The discrepancy was explained by the cytoenzymatic reaction to Beta-GR. The reaction product was visible in the vesicular structures, situated above the plasmolemma, although some of them contained no reaction product. Thus the presence of two types of secretory structure can be inferred: the mucocysts, with ATP-ase in their membranes, and other extrusomes containing active Beta-GR.
3

A comparison of nucleic acid content in Balantidium coli, trophozoites from different isolates

63%
Skotarczak B., Zielinski R.
Folia Biologica
|
1997
|
vol. 45
121-124
EN
Cytophotometric assays were performed on Balantidium coli trophozoites isolated from 30 pigs affected by acute balantidiasis (Group I) and from 30 pigs with symptom-free balantidiasis (Group II). Trophozoites from cultures obtained from Group I and II pig isolates were assayed for comparison. Comparative cytophotometric studies on nucleic acids of B.coli trophozoites isolated from acute and symptomless balantidiasis-affected pigs as well as from in vitro cultured trophozoites showed differences which could have resulted from diferences between populations in the trophozoans under investigation.
4

The occurrence DNA of Babesia microti in ticks Ixodes ricinus in the forest areas of Szczecin

63%
Skotarczak B., Cichocka A.
Folia Biologica
|
2001
|
vol. 49
|
issue 3-4
247-250
EN
Babesia microti, an intraerythrocytic protozoan and the etiological agent of human babesiosis, is transmitted by the bite of the tick, Ixodes ricinus. The aim of the present study was to confirm the presence of B. microti by detection of the DNA of these protozoans. The prevalence of B. microti was studied using the PCR method with primers complementary to the gene fragment encoding nuclear small-subunit ribosomal RNA (ss-rDNA). In the course of this study a total of 2095 ticks, Ixodes ricinus, were examined. The mean infection rate was 6.2%. Variable prevelance values were also obtained from six different locations and they were further modified by the seasons of the year. The results confirmed the competence of I. ricinus as a vector of B. microti and that a B. microti-specific PCR can provide a sensitive test for laboratory detection of babesiosis.
5

Phylogenetic relationships of Paramecium jenningsi strains (classical analysis and RAPD studies)

51%
Przybos E., Skotarczak B., Wodecka B.
Folia Biologica
|
2003
|
vol. 51
|
issue 1-2
85-95
EN
The present studies with application of classical strain crosses and RAPD-PCR analyses showed the existence of different genetic species (syngens) within Paramecium jenningsi. So far the existence of only one syngen has been accepted. It was found that strains from Saudi Arabia, India, and China compose one genetic species (syngen 1) and six strains from Japan compose second genetic species (syngen 2).
6

Random amplified polymorphic DNA fingerprinting as a marker for Paramecium jenningsi strains

51%
Skotarczak B., Przybos E., Wodecka B., Maciejewska A.
Folia Biologica
|
2004
|
vol. 52
|
issue 1-2
117-124
EN
The aim of the present study is to establish a common RAPD marker for P. jenningsi using a series of Ro primers and to investigate if strains originating from distant and isolated localities (Japan, China, India, Saudi Arabia) have isolated gene pools and represent distinct species. An analysis of dendrograms constructed on the basis of RAPD-PCR fingerprints with four primers (Ro 460-04, 460-06, 460-07, and 460-10) from the first part of this project (SKOTARCZAK et al. 2004), assigns the strains to two groups consisting of the continental strains (India, Saudi Arabia, China) and Japanese strains that have been considered as a separate sibling species within P. jenningsi. The genetic similarity of the Indian and Arabian strains was ascertained, whereas the Chinese strain formed an independent branch in this sibling species. The primers Ro (460-01, 460-02, 460-03, 460-05, 460-08) also distinguish between two groups of strains, although they divide the Japanese strains into two subgroups that are not reproductively isolated. This probably indicates genetic variation within this sibling species. However, it comprises one common gene pool (successful inter-strain crosses) and is reproductively isolated from the other sibling species. The results presented in these papers confirm that the construction of ten band patterns having marker attributes is possible on the basis ofDNAamplification from 9 strains of P. jenningsi with the RAPD-PCR fingerprinting method using five primers from the Ro series. The patterns can be assigned to three marker-groups: a general species group, a group differentiating between sibling species, and accessory strain markers.
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