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Methodology for FTIR Imaging of Individual Cells

100%

Yao S., Cestelli Guidi M., Delugin M., Della-Ventura G., Marcelli A., Petibois C.

Acta Physica Polonica A

2016

vol. 129

issue 2

250-254

FTIR imaging is a novel spectroscopic technique able to provide cell imaging, in vivo and in real-time. However, one key issue is developing methodologies for cell culture on IR-transparent substrates fitting cell biology requirements. In this work we tested different IR-transparent substrates in terms of biotoxicity, surface properties, and spectral image acquisition qualities. Only a few substrates, namely Si₃N₄, Ge, GLS, LaF₃, Si, SrF₂, ZnS/C, ZnS/F, were found to provide cell culture conditions comparable to those observed on usual polycarbonate Petri dishes, the main limiting parameter being the toxicity of the material (ZnS, GLS, PbF₂, PbCl₂) or a poor adhesiveness (notably diamond, AgCl, CaF₂, ZnS). From substrates eligible for a good-quality cell culture, the spectral acquisition quality is mainly affected by the refractive index value. Finally, the best compromise between cell culture quality and image spectral quality could be obtained using Si and Ge substrates. This rationalization of the available IR-transparent substrates for bioimaging is particularly relevant for live cell analyses, where cell culture conditions must remain unaffected by substrate properties.

Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis

84%

Kwiatek W., Paluszkiewicz C., Banaś A., Kisiel A., Podgórczyk M., Marcelli A., Cestelli Guidi M., Piccinini M.

Acta Physica Polonica A

2009

vol. 115

issue 2

602-605

The infrared spectromicroscopy is a quite recent technique rapidly developing thanks to the availability of new instruments and new brilliant synchrotron radiation sources in different areas and in particular to biomedical researches. In order to achieve a diffraction limited spatial resolution in tissue samples, we performed experiments at SINBAD, the synchrotron infrared beamline of the Laboratori Nazionali di Frascati. We characterized the chemical composition of prostate tissue samples taken from patients affected by prostate cancer disease. Different sizes of the pinholes were considered for the measurements. In the case of prostate tissue sections the results show the possibility to determine the intensity ratio of the CH_2 and CH_3 bands set at 2930 cm^{-1} and 2960 cm^{-1}, respectively. Experiments were also performed with a pinhole of 5 μm of diameter and the differences in both histological and chemical compositions of such samples were determined.

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2 Acta Physica Polonica A

1 2016

1 2009

2 Cestelli Guidi M.

2 Marcelli A.

1 Banaś A.

1 Della-Ventura G.

1 Delugin M.

1 Kisiel A.

1 Kwiatek W.

1 Paluszkiewicz C.

1 Petibois C.

1 Piccinini M.

1 Podgórczyk M.

1 Yao S.

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Methodology for FTIR Imaging of Individual Cells

Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis