Search results

1

High-Density Cultivation of the Marine Ciliate Uronema marinum (Ciliophora, Oligohymenophorea) in Axenic Medium

100%

ZHENG W., Gao F., Warren A.

Acta Protozoologica

|

2015

|

vol. 54

|

issue 4

PL

Uronema marinum is a cosmopolitan marine ciliate. It is a facultative parasite and the main causative agent of outbreaks of scuticociliatosis in aquaculture fish. This study reports a method for the axenic cultivation of U. marinum in high densities in an artificial medium comprising proteose peptone, glucose and yeast extract powder as its basic components. The absence of bacteria in the cultures was confirmed by fluorescence microscopy of DAPI-stained samples and the failure to recover bacterial SSU-rDNA using standard PCR methods. Using this axenic medium, a maximum cell density of 420,000 ciliate cells/ml was achieved, which is significantly higher than in cultures using living bacteria as food or in other axenic media reported previously. This method for high-density axenic cultivation of U. marinum should facilitate future research on this economically important facultative fish parasite.

2

Phylogenetic Analyses on the Tintinnid Ciliates (Protozoa, Ciliophora) Based on Multigene Sequence Data

100%

Zhao Y., Gao F., Li J., Yi Z.

Acta Protozoologica

|

2012

|

vol. 51

|

issue 4

PL

In order to better understand phylogenetic relationships among tintinnid ciliated protozoa, we sequenced and analyzed the SSU rDNA and ITS1-5.8S-ITS2 regions of 10 species belonging to five genera in the order Tintinnida. The secondary structures of the ITS2 region were compared among 8 closely related genera, revealing two stable helices of the palm. In addition, we identified a bulge absence in position II of the ITS2 putative secondary structures of species in basal positions in phylogenetic trees, suggesting the absence bulge might be an ancestral character in the order Tintinnida. Phylogenetic analyses based on SSU rDNA and ITS1-5.8S-ITS2 regions sequence show 1) divergences within the family Tintinnidae are higher than that among other four families (Codonellidae, Ptychocylididae, Metacylididae and Codonellopsidae), suggesting the subdivision of the this family; 2) the family Ptychocylididae is polyphyletic; 3) the subdivision of Tintinnopsis are suggested, because the Tintinnopsis spp. scatter into different clades; 4) species with agglutinated loricae are not clearly separated from that with hyaline ones.

3

Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.

76%

Pan X., Gao F., MIAO M., Al-Rasheid K. A. S., Ma H., Fan X., Huang J.

Acta Protozoologica

|

2015

|

vol. 54

|

issue 1

PL

The morphology and infraciliature of four marine scuticociliates, Pleuronema elegans spec. nov., P. setigerum Calkins, 1902, P. grolierei Wang et al., 2008 and Uronema orientalis spec. nov., collected from China seas, were investigated through live observation and protargol staining methods. Pleuronema elegans spec. nov. can be recognized by the combination of the following characters: size in vivo 90–115 × 45–60 µm, slender oval in outline with a distinctly pointed posterior end; about 10 prolonged caudal cilia; consistently two preoral kineties and 18 or 19 somatic kineties; membranelle 2a double-rowed with its posterior end straight; membranelle 3 three-rowed; one macronucleus; marine habitat. Uronema orientalis spec. nov. is distinguished by the following features: in vivo about 40–55 × 20–30 μm with a truncated apical plate; consistently twenty somatic kineties; membranelle 1 single-rowed and divided into two parts which comprise four and three basal bodies respectively; contractile vacuole pore positioned at the end of the second somatic kinety; marine habitat. We also provide improved diagnoses for P. grolierei Wang et al., 2008 and P. setigerum Calkins, 1902 based on current and previous reports. The small subunit rRNA gene of U. orientalis, P. elegans, P. grolierei and P. puytoraci were sequenced. Phylogenetic analyses indicate that Uronema and Pleuronema are not monophyletic.

Refine search results

3 Acta Protozoologica

2 2015

1 2012

3 Gao F.

1 Al-Rasheid K. A. S.

1 Fan X.

1 Huang J.

1 Li J.

1 MIAO M.

1 Ma H.

1 Pan X.

1 Warren A.

1 Yi Z.

1 ZHENG W.

1 Zhao Y.

High-Density Cultivation of the Marine Ciliate Uronema marinum (Ciliophora, Oligohymenophorea) in Axenic Medium

Phylogenetic Analyses on the Tintinnid Ciliates (Protozoa, Ciliophora) Based on Multigene Sequence Data

Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.