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1

DNAzyme as an efficient tool to modulate invasiveness of human carcinoma cells

100%

Wiktorska M., Papiewska-Pająk I., Okruszek A., Sacewicz-Hofman I., Niewiarowska J.

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2010

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vol. 57

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issue 3

269-275

EN

In this study we evaluated efficiency of DNAzymes to modulate motility of cancer cells, an important factor in the progression and metastasis of cancers. For this purpose we targeted β1 integrins that are predominant adhesive receptors in various carcinoma cell lines (CX1.1, HT29, LOVO, LS180, PC-3). To evaluate invasiveness of cancer cells, we used a transwell migration assay that allowed analyzing chemotactic migration of colon carcinoma cell lines across an ECM-coated membrane. Their adhesive properties were also characterized by the analysis of adhesion to fibronectin, laminin and collagen. In addition, the expression of major integrin subunits, selected intact β1 integrins, and other adhesive receptors (ICAM, E-selectin, uPAR) was analyzed by flow cytometry. Inhibition of β1 integrin expression by DNAzyme to β1 mRNA almost abolished the invasiveness of the CX1.1, HT29, LS180, LOVO and PC-3 cells in vitro. These data show that DNAzymes to β1 integrin subunit can be used to inhibit invasiveness of carcinoma cells.

2

Synthesis and interaction with thymidylate synthase of 5'-dithiophosphate and 5'-fluorothiophosphate of thymidine

100%

Gołos B., Misiura K., Olesiak M., Okruszek A., Stecand Wojciech Rode W. J.

Acta Biochimica Polonica

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1998

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vol. 45

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issue 1

83-86

3

Diastereomers of thymidine 3’-O-(methanephosphonothioate): Synthesis, absolute configuration and reaction with 3'-methoxyacetylthymidine under conditions of triester approach to oligonucleotide synthesis

86%

Niewiarowski W., Leśnikowski Z. J., Wilk A., Guga P., Okruszek A., Uzański B., Stec W.

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issue 2

217-231

4

The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E. coli, purification and ESR studies of its interaction with human blood platelets

73%

Pietrucha T., Stec W. J., Okruszek A., Uznański B., Koziołkiewicz M., Wilk A., Płucienniczak A., Świątkowska M., Cierniewski C. S.

Acta Biochimica Polonica

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1994

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vol. 41

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issue 1

25-34

5

Inhibition of plasminogen activator inhibitor release in endothelial cell cultures by antisense oligodeoxyribonucleotides with a 5˘-end lipophilic modification

59%

Kobylańska A., Pluskota E., Światkowska M., Wójcik M., Cierniewska-Cieślak A., Krakowiak A., Boczkowska M., Pawłowska Z., Okruszek A., Koziołkiewicz M., Cierniewski C. S., Stec W. J.

Acta Biochimica Polonica

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1999

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vol. 46

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issue 3

679-691

Refine search results

DNAzyme as an efficient tool to modulate invasiveness of human carcinoma cells

Synthesis and interaction with thymidylate synthase of 5'-dithiophosphate and 5'-fluorothiophosphate of thymidine

Diastereomers of thymidine 3’-O-(methanephosphonothioate): Synthesis, absolute configuration and reaction with 3'-methoxyacetylthymidine under conditions of triester approach to oligonucleotide synthesis

The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E. coli, purification and ESR studies of its interaction with human blood platelets

Inhibition of plasminogen activator inhibitor release in endothelial cell cultures by antisense oligodeoxyribonucleotides with a 5˘-end lipophilic modification