Search results

Sort By:

Limit search:

Thermostability conditions and catalytic characterization of isocitrate dehydrogenase from Bacillus licheniformis

100%

Zabielski P.

Biotechnologia

2001

issue 3

219-230

The NADP+ - dependent isocitrate dehydrogenase (IDH) from Bacillus licheniformis was partially purified using ammonium sulphate fractionation and gel filtration on Sephadex G-200 column. The enzyme preparation had specific activity of 1.52 U mg-1. The temperature optimum for the IDH activity was about 59C. The Arrthenius activation energy was determined to be 65.3 kJ/mol below 47C, and 18.3 kJ/mol above this temperature. The IDH activity at 65C was much protected by isocitrate and magnesium, but no NADP+. Manganese ions were more efficient activators of the enzyme than Mg2+. Calcium ions rather inhibited the IDH. The Km values for DL-isocitrate and NADP+ in phosphate buffer (pH 7.4) at 20C were 85.5 and 4.9 muM, respectively; at 58C the corresponding values were 181.5 and 69.3 muM.

Liver regeneration ofter partial hepatectomy. Function of the lipid messengers

51%

Chocian G., Zabielski P., Chabowski A.

Postępy Higieny i Medycyny Doświadczalnej

2002

vol. 56

issue 1

49-71

The paper contains current data on the different ways of the liver regeneration. There is still not enough data about control of the regeneration processes in the liver. However nuclear phosphatidylinositols and sphingomyelins have been shown to play a potent role of messengers signaling. The lipid messengers may be potent factors affecting process of liver regeneration after partial hepatectomy.

Refine search results

1 Biotechnologia

1 Postępy Higieny i Medycyny Doświadczalnej

1 2002

1 2001

2 Zabielski P.

1 Chabowski A.

1 Chocian G.

Search results

Thermostability conditions and catalytic characterization of isocitrate dehydrogenase from Bacillus licheniformis

Liver regeneration ofter partial hepatectomy. Function of the lipid messengers