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Comparison of two methods used for monitoring low-copy cytomegalovirus infection in a patient with chronic myeloid leukemia after unrelated umbilical cord blood transplantation

100%

Dzieciatkowski T., Przybylski M., Tomaszewska A., Rokicka M., Luczak M.

Archivum Immunologiae et Therapiae Experimentalis

2007

vol. 55

issue 3

199-203

Introduction: Detection of human cytomegalovirus (CMV, HHV-5) DNA in clinical specimens is considered a cornerstone in the diagnosis of HHV-5 disease. The present study compared two quantitative methods used for diagnosing cytomegalovirus infection in a 21-year-old woman with chronic myeloid leukemia after an unrelated umbilical cord blood transplantation. Materials and Methods: Blood samples were tested for the presence of HHV-5 DNA using the LightCycler PCR, the quantitative Eclipse? CMV DNA Detection Kit, and a qualitative in-house PCR assay using primers that amplify part of the HHV-5 MIE gene. Results: Results from samples containing a low cytomegalovirus load were more accurate with the LightCycler test than those obtained with the Eclipse? test, which underestimated the viral load of samples containing low DNA copy numbers. Conclusions: These findings underline the value of novel PCR methods used in current therapeutic procedures and in monitoring antiviral therapy with nucleoside analogs. The high level of sensitivity, specificity, accuracy, and rapidity provided by the LightCycler instrument are favorable for the use of this system in the detection of HHV-5 DNA in clinical specimens.

Simultaneous transplantation of two allogeneic units of cord blood in an adult patient with acute myeloblastic leukemia. A case report

59%

Wiktor-Jedrzejczak W., Rokicka M., Urbanowska E., Torosian T., Graczyk-Pol E., Tomaszewska A., Krol M., Paluszewska M., Gronkowska A., Ziarkiewicz-Wroblewska B., Jolkowska J., Witt M.

Archivum Immunologiae et Therapiae Experimentalis

2005

vol. 53

issue 4

364-368

The major obstacle to the therapeutic use of hematopoietic transplantation is the unavailability of matched, unrelated marrow donors for the large number of potential patients, although all of them have the chance to find sufficiently matched, unrelated cord blood units. However, the use of cord blood as a source of cells for transplantation is limited by its cell number, usually below 1 billion, which allows for routine transplantation only in children weighting less than 30 kg, while most potential recipients possess a higher body mass. This led to the idea of the simultaneous use of several units of cord blood which, combined, would fulfill the requirements for the necessary cell number for an adult recipient. We attempted to simultaneously transplant an adult patient with refractory acute myeloblastic leukemia utilizing two different cord blood units, one fully matched and one mismatched at one locus. The patient became reconstituted with only one unit, the mismatched, as determined using microsatellite markers, and had no signs of relapse of leukemia. Unfortunately, he died of persistent fungal (brain aspergilloma) infection on day +103. The successful engraftment may suggest that a method based on the principle of using more than one cord blood unit for transplantation is feasible in large adult patients and may reach routine application.

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2 Archivum Immunologiae et Therapiae Experimentalis

1 2007

1 2005

2 Rokicka M.

2 Tomaszewska A.

1 Dzieciatkowski T.

1 Graczyk-Pol E.

1 Gronkowska A.

1 Jolkowska J.

1 Krol M.

1 Luczak M.

1 Paluszewska M.

1 Przybylski M.

1 Torosian T.

1 Urbanowska E.

1 Wiktor-Jedrzejczak W.

1 Witt M.

1 Ziarkiewicz-Wroblewska B.

Search results

Comparison of two methods used for monitoring low-copy cytomegalovirus infection in a patient with chronic myeloid leukemia after unrelated umbilical cord blood transplantation

Simultaneous transplantation of two allogeneic units of cord blood in an adult patient with acute myeloblastic leukemia. A case report