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Application of modern methods for plant material studies of Gentiana kurroo (Royle) somatic embryogenesis

100%
Fiuk A., Rybczynski J. J.
Biotechnologia
|
2006
|
issue 4
95-106
EN
Nowadays somatic embryogenesis is being investigated with special attention paid to the identification of genes directly involved in triggering of cell competence and development of ontogenic stages. In order to trace successive cell divisions that lead to the formation of the globular structures, modern scanning and electron microscopy methods are applied. Plantlets, which come from somatic embryogenesis process, should be exactly the same as mother plants but in vitro culture conditions may induce many disturbances, which could be lasting (hereditary) or only transitory. These changes are usually called somaclonal variation and could be observed on different levels of the plant organization. To investigate this kind of variations both of the genetic and epigenetic types, specially designed molecular systems are needed. Here, we describe induction of somatic embryos from several explants of G. kurroo. In order to evaluate the particular ontogenic stages of somatic embryos and variability of regenerated plants, following methods were applied: light microscopy, scanning and transmission electron microscopy, 2D protein electrophoresis, flow cytometry of DNA content in the cell nucleus, cytogenetic analysis of chromosome number and molecular analysis with the use of AFLP.
2

In vitro culture of Gentiana kurroo (Royle)

81%
Fiuk A., Rajkiewicz M., Rybczynski J. J.
Biotechnologia
|
2003
|
issue 3
267-274
EN
The aim of the presented paper is to show the present status of in vitro studies of Gentiana kurroo (Royle). Experimental material originated from seedlings, organs of regenerants, callus tissue, cell suspensions and isolated protoplasts. Two years of experiments proved very high morphological potential of plant material originated from various system of cultures. The mostly used media were those based on Murashige and Skoog medium, but for protoplast cultures, Kao and Michaluk medium appeared to be most useful. Numerous plant growth regulators with various concentrations and combinations controled plant regeneration of studied gentiana. Plant regeneration via somatic embriogenesis was observed in the majority of the studied culture systems.
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