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Testing endodontic sealers on human umbilical vein endothelial cells

100%
Moldovan M., Filip A., Prejmerean C., Prodan D., Alb C., Pop A., Ioana B.
Open Medicine
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2013
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vol. 8
|
issue 4
523-529
EN
The toxic potential of the endodontic sealers ingredients, especially the unreacted monomer, that can irritate the periapical tissue and interfere with the healing process, thus having a negative impact on the biocompatibility of the material. The aim of this study was to evaluate the influence of three experimental endodontic sealers on cells viability in vitro. Human umbilical vein endothelial cells (HUVEC) were used. The experiments were done with solid samples and extracts of sealers in artificial saliva and water. The experiments evaluated the cytotoxicity of the residual monomers that resulted from the tested composites. The decrease in cell viability was quantified by colorimetric measurement of formazan. The components of the sealers dissolved in artificial saliva and water were determined by high performance liquid chromatography (HPLC). The HUVEC are a novelty for testing the endodontic sealers biocompatibility, with certain advantages compared to other cell types used in the literature, e.g. HELA cells, fibroblasts. The data showed that cytotoxicity was directly linked with the unreacted monomer - 2-hydroxyethyl methacrylate (HEMA) present in these composites. Two of the three formulations had little or no cytotoxic effect, which makes them suitable for further testing in order to be used in endodontic treatment.
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Structural and morphological properties of HA-ZnO powders prepared for biomaterials

84%
Moldovan M., Prodan D., Popescu V., Prejmerean C., Saroși C., Saplonţai M., Țălu S., Vasile E.
Open Chemistry
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2015
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vol. 13
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issue 1
EN
The purpose of this study was to investigate the structural and morphological properties of hydroxyapatite – zinc oxide (HA-ZnO) powders prepared to be used in an endodontic sealer formulation. The HA-ZnO was synthesized from hydroxyapatite (HA) in the presence of zinc oxide (ZnO) sol. The starting reagents were CaO, H3PO4 and a suspention of ZnO (25% in water). Following precipitation, the obtained sol was subjected to heat treatment at 110°C, 400°C, 850°C and 1050°C. The study focused on the influence of the thermal treatment on the cristallinity of precipitated powders. X-ray diffraction was used in order to study the structural properties of the obtained powder. Fourier Transform Infrared Spectroscopy (FT-IR) was used in order to evaluate the interactions between HA and ZnO. The morphology of the samples was studied by Scanning Electron Microscopy (SEM). Transmission Electron Microscopy and High Resolution Transmission Electron Microscopy (TEM, HRTEM) were used for the determination of particle size and fractal analysis. The fractal analysis of images using the standard box-counting method is presented and the results are discussed. It was demonstrated that the fractal dimension analysis is a useful method to quantitatively describe the complex microstructures and it can reveal the relation between fractal parameters.
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