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1

The effect of salicylic acid, jasmonates biosynthesis inhibitor, on cell suspension growth, development and regeneration of somatic embryos in Medicago sativa L. tissue cultures

100%
Kepczynska E., Zielinska S.
Biotechnologia
|
2004
|
issue 2
114-121
EN
The effect of salicylic acid (SA) applied during the proliferation phase of cell suspension in vitro regeneration system of Medicago sativa L. on cell suspension growth, production of somatic embryos and their following development, germination and conversion were examined. SA is a potent inhibitor of the above processes. It is suggested that endogenous jasmonates are of some importance in regulation of somatic embryogenesis.
2

Ethylene and bacteria

81%
Kepczynska E., Zielinska S., Kepczynski J.
Biotechnologia
|
2000
|
issue 1
147-155
EN
The review article presents data on the ethylene emanation by bacteria, the two different pathways of its biosynthesis in these microorganisms and the role of ethylene in plant pathogenesis. ACC deaminase from Pseudomonas and Enterobacter spp., which catalyses the hydrolytic cleavage of ACC in higher plants was also discussed.
3

The effects of 2,4-D, NAA and picloram on callus induction and somatic embryogenesis in ornamental asparagus

81%
Kepczynski J., Zielinska S., Kepczynska E.
Biotechnologia
|
2001
|
issue 2
170-173
EN
In this study we examined the effect of 2,4-D, NAA and picloram at 1.5, 3.0 and 4.5 mg/l in MS medium on callus induction and somatic embryogenesis in Asparagus densiflorus cv. Sprengeri and Asparagus plumosus. The callus formation of both shoot tips and nodal explants from A. densiflorus after 8 weeks of culture on MS medium supplemented with NAA and picloram (1.5, 3.0 mg/l) was observed. However, callus on medium containing 2,4-D was occasionally induced. On medium supplemented with picloram shoot tip explants of Asparagus plumosus showed callus inducing capacity. After four weeks of callus culture on hormone-free medium the globular embryos were achieved. On callus induced NAA organogenesis was observed.
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