Search results

Sort By:

Limit search:

Serological differentiation of H. pylori CagA(+) and CagA(-) infections

100%

Chmiela M., Wisniewska M., Bak-Romaniszyn L., Rechcinski T., Planeta-Malecka I., Bielanski W., Konturek S. J., Plonka M., Klink M., Rudnicka W.

Archivum Immunologiae et Therapiae Experimentalis

2003

vol. 51

issue 2

131-136

Many of H. pylori strains causing gastroduodenal diseases have a cagA gene encoding CagA protein, a virulence factor of these bacteria. Anti-CagA antibodies produced by majority of people infected with CagA(+) strains can indicate such infection. In this study the efficacy of three immunoenzymatic tests: immunoblot (MileniaID Blot H. pylori IgG, DPC Biermann GmbH, Germany) (MB) and ELISA, conducted with a recombinant immunodominant fragment of CagA (rCagA) and full length CagA molecule (flCagA), in detecting CagA(+) and CagA(-) infections, was compared. The 13C urea breath test (13C-UBT) was used for establishing H. pylori status. The serum samples from 157 individuals were used for serodiagnosis. The H. pylori CagA(+) infection was detected in H. pylori infected individuals with similar frequency by MB (64%) and flCagA-ELISA (60%) and little less frequently by rCagA-ELISA (53%). There was a high coincidence between the negative results of these three tests for H. pylori uninfected individuals with no anti-CagA IgG in the serum (96-100%). The results show that rCagA-ELISA and especially flCagA-ELISA are easy, inexpensive and useful noninvasive assays for discrimination of CagA(+) and CagA(-) H. pylori infections in the subjects examined by urea breath test.

Refine search results

1 Archivum Immunologiae et Therapiae Experimentalis

1 2003

1 Bak-Romaniszyn L.

1 Bielanski W.

1 Chmiela M.

1 Klink M.

1 Konturek S. J.

1 Planeta-Malecka I.

1 Plonka M.

1 Rechcinski T.

1 Rudnicka W.

1 Wisniewska M.

Search results

Serological differentiation of H. pylori CagA(+) and CagA(-) infections