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Triple-stranded forms of nucleic acids

100%

Kwinkowski M.

Biotechnologia

1994

issue 4

69-78

Triple-stranded DNA structures are presented.Methods for their analysis and their physical and biological properties are reviewed.Application of triplex-forming oligonucleotides as agents for anti-gene therapy is discussed.

Antisense oligonucleotides against inducible nitric oxide synthase reduce No production in RAW 264.7 cells

45%

Bilecki W., Okruszek A., Kwinkowski M., Sanak M., Przewlocki R.

Biotechnologia

1996

issue 4

173-182

Several oligodeoxyribonucleotides in a nuclease-resistant phosphorothioate form targeted to iNOS mRNA were tested in RAW 264,7 cells as potential antisense inhibitors.Antisense S-ODNs inhibited iNOS activity in a time- and dose-depended manner.Application of Lipofectin agent, which itself had no significant effect, greatly increased antisense activity.Decreased levels of the target mRNA, as demonstrated by reverse transcriptase -polymerase chain reaction (RT-PCR), suggest tha RNase H mediated mechanism.

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2 Biotechnologia

1 1996

1 1994

2 Kwinkowski M.

1 Bilecki W.

1 Okruszek A.

1 Przewlocki R.

1 Sanak M.

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Triple-stranded forms of nucleic acids

Antisense oligonucleotides against inducible nitric oxide synthase reduce No production in RAW 264.7 cells