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PCR-RFLP detection of point mutations A2143G and A2142G in 23S rRNA gene conferring resistance to clarithromycin in Helicobacter pylori strains

100%

Klesiewicz K., Nowak P., Karczewska E., Skiba I., Wojtas-Bonior I., Sito E., Budak A.

2014

vol. 61

issue 2

311-315

Background. The occurrence of clarithromycin resistance among Helicobacter pylori strains is a major cause of the treatment failure. Resistance to this drug is conferred by point mutations in 23S rRNA gene and the most prevalent mutations are A2143G and A2142G. The aim of the study was to evaluate the occurrence of A2143G and A2142G mutations in a group of H. pylori strains resistant to clarithromycin. Materials and Methods. The study included 21 clarithromycin-resistant H. pylori strains collected between 2006 and 2009 in southern Poland. Resistance to clarithromycin was quantitatively tested with the E-test to determine the minimal inhibitory concentration (MIC value). The point mutations of H. pylori isolates were detected by PCR followed by RFLP analysis. Results. The MIC values for clarithromycin for the analyzed strains ranged from 1.5 mg/L to 64 mg/L. Nine H. pylori strains exhibited A2143G mutation and A2142G mutation was found in 9 isolates as well. The results of RFLP analysis of 3 clarithromycin-resistant strains were negative for both mutations. The average MIC values for A2143G and A2142G mutants were 6 and 30 mg/L, respectively. Conclusions. Frequencies of A2143G and A2142G mutations were the same in all isolates tested. Strains with A2143G mutation exhibited lower MIC values than A2142G mutants. Application of PCR-RFLP method for detection of clarithromycin resistance allows for better and more efficient management of H. pylori infections.

COMPARATIVE IN VITRO STUDIES OF FURAZIDIN AND NITROFURANTOIN ACTIVITIES AGAINST COMMON UROPATHOGENS INCLUDING MULTIDRUG-RESISTANT STRAINS OF E. COLI AND S. AUREUS

84%

Klesiewicz K., Karczewska E., Nowak P., Mrowiec P. M., Skiba-Kurek I., Białecka J., Majka Z., Berdzik-Kalarus S., Budak A., Zajdel P.

Acta Poloniae Pharmaceutica - Drug Research

2018

vol. 75

issue 3

Abstract: Urinary tract infections caused by wide range of pathogens including gram-negative and gram-positive bacteria as well as fungi are a severe public health problem. The predominant causative agent of both uncomplicated and complicated urinary tract infections is Escherichia coli. In an era of increasing bacterial resistance to antimicrobial agents and a high prevalence of multidrug-resistant (MDR) strains in community and hospital acquired infections, the re-evaluation of older generations of antimicrobial agents, such as nitrofuran derivatives, seems to be a reasonable approach. The aim of the study was to evaluate furazidin activity against common uropathogens in comparison to nitrofurantoin and other selected antimicrobial agents, routinely used in the treatment of urinary tract infections. Furazidin exhibited lower MICs than nitrofurantoin when tested against gram-negative and gram-positive bacteria including clinical MDR E. coli and methicillin-resistant Staphylococcus aureus. The MICs for furazidin ranged from 4 to 64 mg/L for Enterobacteriaceae strains, from 2 to 4 mg/L for gram-positive cocci, and 0.5 mg/L for anaerobic bacteria. The MICs for nitrofurantoin ranged from 16 to 64 mg/L for Enterobacteriaceae strains, from 8 to 64 mg/L for gram-positive cocci, and 4 mg/L for anaerobic bacteria. In addition, both nitrofurans displayed better activity against the tested bacterial strains than ciprofloxacin, fosfomycin, trimethoprim and co-trimoxazole. Nitrofuran derivatives displayed higher antimicrobial activity than other antimicrobial agents regardless of bacteria species or resistance mechanism.

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1 Acta Biochimica Polonica

1 Acta Poloniae Pharmaceutica - Drug Research

1 2018

1 2014

2 Budak A.

2 Karczewska E.

2 Klesiewicz K.

2 Nowak P.

1 Berdzik-Kalarus S.

1 Białecka J.

1 Majka Z.

1 Mrowiec P. M.

1 Sito E.

1 Skiba I.

1 Skiba-Kurek I.

1 Wojtas-Bonior I.

1 Zajdel P.

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PCR-RFLP detection of point mutations A2143G and A2142G in 23S rRNA gene conferring resistance to clarithromycin in Helicobacter pylori strains

COMPARATIVE IN VITRO STUDIES OF FURAZIDIN AND NITROFURANTOIN ACTIVITIES AGAINST COMMON UROPATHOGENS INCLUDING MULTIDRUG-RESISTANT STRAINS OF E. COLI AND S. AUREUS