Search results

Sort By:

Limit search:

Cystic fibrosis ? a probable cause of Frederic Chopin?s suffering and death

100%

Majka L., Gozdzik J., Witt M.

Journal of Applied Genetics

2003

vol. 44

issue 1

77-84

Frederic Chopin ? a great Polish composer and pianist ? suffered from a chronic disease. Both during his life and after death physicians disagreed on the subject of Chopin?s diagnosis. His contemporaries accepted the diagnosis of a disease common in the 18th century ? tuberculosis. Description of new clinical entities provoked new dilemmas in the 20th century. In our opinion the most tenable seems to be the diagnosis of cystic fibrosis. In this work we present F. Chopin?s case history and discuss cons and pron for cystic fibrosis as the cause of F. Chopin?s suffering and death.

The migration of bone marrow-derived non-hematopoietic tissue-committed stem cells is regulated in an SDF-1-, HGF-, and LIF-dependent manner

64%

Kucia M., Wojakowski W., Reca R., Machalinski B., Gozdzik J., Majka M., Baran J., Ratajczak J., Ratajczak M. Z.

Archivum Immunologiae et Therapiae Experimentalis

2006

vol. 54

issue 2

121-135

Introduction: Recently we identified in bone marrow (BM) by employing chemotactic isolation to SDF-1 gradient combined with real time RT-PCR analysis a mobile population of CXCR4+ BM mononuclear cells that express mRNA for various markers of early tissue-committed stem cells (TCSCs). In this study we evaluated whether TCSCs respond to other moto-morphogens, such as hepatocyte growth factor (HGF) and leukemia inhibitory factor (LIF). Materials and Methods: We again employed chemotactic isolation combined with real-time RT-PCR analysis to assess whether murine and human BM contain TCSCs that respond to HGF and LIF gradients. We also evaluated expressions of HGF and LIF in damaged organs. Results: We noted that the number of TCSCs is highest in BM from young (1- to 2-month-old) mice and decreases in 1-year-old animals. Murine and human TCSCs 1) respond to HGF and LIF gradients in addition to an SDF-1 gradient, 2) reside in populations of BM-derived non-hematopoietic CD45? cells, and 3) are released (mobilized) from BM into the peripheral blood (PB) during tissue injury (e.g. after partial body irradiation). Conclusions: These findings further support our theory of the BM as a ?hideout' for TCSCs and we suggest that their presence in BM tissue should be considered before experimental evidence is interpreted simply as transdifferentiation/plasticity of hematopoietic stem cells. Since we demonstrated that not only SDF-1, but also HGF and LIF are upregulated in damaged tissues, we postulate that CXCR4+ c-Met+ LIF-R+ TCSC could be mobilized from the BM into the PB, from which they are subsequently chemoattracted to damaged organs, where they play a role in tissue repair/regeneration.

Refine search results

1 Archivum Immunologiae et Therapiae Experimentalis

1 Journal of Applied Genetics

1 2006

1 2003

2 Gozdzik J.

1 Baran J.

1 Kucia M.

1 Machalinski B.

1 Majka L.

1 Majka M.

1 Ratajczak J.

1 Ratajczak M. Z.

1 Reca R.

1 Witt M.

1 Wojakowski W.

Search results

Cystic fibrosis ? a probable cause of Frederic Chopin?s suffering and death

The migration of bone marrow-derived non-hematopoietic tissue-committed stem cells is regulated in an SDF-1-, HGF-, and LIF-dependent manner