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1
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Partial purification and some properties of a liver alkaline ribonuclease from the frog Rana esculenta

100%
Malicka-Błaszkiewicz M., Kubicz A.
Acta Biochimica Polonica
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1979
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vol. 26
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issue 3
275-283
2
Content available remote

Acid phosphatase from liver of the frog Rana esculenta, separation and partial characterization of multiple forms

81%
Kubicz A., Dratewka E., Malicka-Błaszkiewicz M.
Acta Biochimica Polonica
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1978
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vol. 25
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issue 4
349-359
3
Content available remote

The effect of methotrexate on actin and invasiveness of hepatoma Morris 5123 cells in culture.

81%
Otrocka M., Verschueren H., Malicka-Błaszkiewicz M.
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2001
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vol. 48
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issue 4
1051-1060
EN
Monomeric (G), total (T) and filamentous (F) actin and the state of actin polymerisation (F:G) were determined and actin filaments were visualized in hepatoma Morris 5123 cells cultured in the presence of methotrexate (MTX) at various concentration. The exposure of the cells to this drug resulted in a decrease of total and polymerised actin in cytoplasm and in some changes in actin filament organization. This coincided with a decrease of the cells' ability to migrate through Matrigel coated filters and with inhibition of tumour formation after reimplantation of the methotrexate treated cells to experimental rats.
4
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Beta-actin in human colon adenocarcinoma cell lines with different metastatic potential.

81%
Nowak D., Skwarek-Maruszewska A., Zemanek-Zboch M., Malicka-Błaszkiewicz M.
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2005
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vol. 52
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issue 2
461-468
EN
Human colon adenocarcinoma LS180 parental cell line and selected variants, characterized by different metastatic capacity were used to examine, whether a correlation exists between β-actin expression, its subcellular distribution and metastatic potential of these cells. Cytosolic fraction (supernatant 105000 × g), isolated from the tumor cells was used as a source for actin quantification. The higher level of β-actin was observed in the cytosol of three selected sublines to compare with LS180 parental line. Statistically significant increase of β-actin level in highly motile EB3 cells variant should be underlined to compare with the other sublines. Distinct differences in the phenotype of adenocarcinoma cell variants were found, such as the changes in cells shape, cells spreading and ability to attach to the surface of culture dish. Actin cytoskeleton was visualized with fluorescence microscopy application and microfilaments rhodamine-conjugated phalloidin staining. β-actin subcellular localization was done by immunofluorescence staining with monoclonal anti-β actin antibodies. In the elongated cells (LS180, 3LNLN), this isoactin is dispersed in the whole cell body and concentrates in pseudopods and at the leading edges, when in the rounded variant (EB3) β-actin dominates mainly in cortical ring under cellular membrane and it is also seen in the subtle protrusions. Summary of our former (Nowak et al., 2002, Acta Biochim. Polon., 49: 823) and current data lead to the conclusion that there is a distinct correlation between metastatic capacity of examined human colon adenocarcinoma cells, the state of actin polymerization, actin cytoskeleton organization and β-actin expression.
5
Content available remote

Identification of actin from hepatoma Morris 5123 cells

81%
Nowak D., Kochman A., Malicka-Błaszkiewicz M.
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EN
The hepatoma Morris 5123 tumor growth is accompanied by changes in actin content and polymerization (Malicka-Błaszkiewicz et al. (1995) Mat. Med. Pol., 27, 115-118;  Nowak et al. (1995) J. Exp. Cancer Res. 14, 37-40). Presently actin isoforms from cytosol and  cytoskeleton fractions were separated by SDS/PAGE and identified with antibodies directed against different actin isoforms. Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit α skeletal muscle actin (M^r 43000) and isoelectric point equal to 5.3. It interacts only with monoclonal anti β actin isoform antibodies, posing the question of differential affinity of actin isoforms to DNase I.
6
Content available remote

Actin in human colon adenocarcinoma cells with different metastatic potential.

81%
Nowak D., Krawczenko A., Duś D., Malicka-Błaszkiewicz M.
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EN
Four human colon adenocarcinoma cell line variants with different metastatic potential were used to examine whether a correlation exists between actin level, state of actin polymerization and invasiveness of tumour cells. Monomeric (G), total (T) and filamentous (F) actin were determined in the cytosolic fraction of these cells. A statistically significant decrease in G actin level and increase in the state of actin polymerization (measured by F:G actin ratio) were found in the cytosol of three cell variants with higher metastatic potential and invasiveness (EB3, 3LNLN, 5W) compared with the parental cell line (LS180). Our experimental data lead to the conclusion that there is a correlation between the metastatic capacity of human colon adenocarcinoma cells and the state of actin polymerization.
7
Content available remote

Does a natural DNase-actin complex exist in the carp liver cytosol?

81%
Krawczenko A., Ciszak L., Malicka-Błaszkiewicz M.
Acta Biochimica Polonica
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1994
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vol. 41
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issue 2
113-116
8
Content available remote

Gelsolin in human colon adenocarcinoma cells with different metastatic potential

71%
Litwin M., Mazur A., Nowak D., Mannherz H., Malicka-Błaszkiewicz M.
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issue 4
739-743
EN
Gelsolin, one of a major actin-binding proteins, is involved in the regulation of actin cytoskeleton organization by its severing and capping activity towards actin filaments. Human colon adenocarcinoma cell line LS180 and its selected variants of different metastatic potential were used to check for a correlation between gelsolin level, its subcellular localization and the invasive capacity of cells. Based on immunoblotting experiments, a decreased level of gelsolin was detected in the most invasive 5W subline when compared to the parental cell line LS180. The intracellular distribution of actin filaments and gelsolin in colon adenocarcinoma cells was examined by confocal microscopy. In the 5W subline, unlike in the other examined cells, gelsolin was colocalized with filamentous actin at the cell periphery. In summary, in human colon adenocarcinoma cells, gelsolin level and its subcellular distribution seem to correlate with their metastatic potential.
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