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1

The transgenes are expressed with different level in plants

100%
Yin Z., Malepszy S.
Biotechnologia
|
2003
|
issue 2
236-260
EN
Variable expression for the same transgene construct has been documented in various plant species, regardless of the type of transgene. This phenomenon and the factors influencing it are reviewed. A variability in the transgene expression level was found to exist between and/or within independently derived lines, different lines of clonal replicates, siblings of the same line, a single plant, a single leaf as well as the same cell. Both the transgene dependent and the recipient dependent factors have been proposed to account for this phenomenon, the transgene dependent factors of which include transgene construct fidelity, T-DNA integration pattern, T-DNA copy number, promoter activity and the effect of nuclear matrix attachment region. In addition, different forms of epigenetic, homology-dependent gene silencing also contribute to the unstable expression of the identical transgene. The recipient dependent factors include position effect, ploidy level, genetic background, homozygosity, and developmental stage. Furthermore, environmental factors such as light intensity, temperature, field growth conditions and the season have been shown to modulate the levels of transgene expression. The approaches to stabilize the transgene expression were also discussed.
2

Differences in the inheritance stability of kanamycin resistance between transgenic cucumbers (Cucumis sativus L.) containing two constructs

81%
Yin Z., Szwacka M., Malinowski R., Malepszy S.
|
|
issue 3
307-314
EN
A phenotypic segregation of kanamycin resistance conferred by nosnptII was investigated in two groups of transgenic cucumber lines one containing the PR-2duidA-nosnptII construct and the other with the thaumatinII-nosnptII construct to the third and fifth generation, respectively. Expression of the nptII gene was more stable and predictable in the progeny of the PR-2d lines than that of thaumatin lines. In T2 and T3 generations representing seven PR-2d primary transformants, 78% of the hemizygous progenies exhibited a segregation ratio consistent with Mendelian inheritance; non-Mendelian ratio occurred in the remaining 22%. Mendelian segregation was observed in 46% of the hemizygous progenies derived from 11 thaumatin primary transformants. The segregation ratio for two and three independent loci appeared in single PR-2d and thaumatin lines, respectively.
3

Transgene inheritance in plants

81%
Yin Z., Plader W., Malepszy S.
Journal of Applied Genetics
|
2004
|
vol. 45
|
issue 2
127-144
EN
The patterns of transgene inheritance in plants and the possible explanations for non-Mendelian transmission are reviewed. The non-Mendelian inheritance of a transgene has been recorded with a frequency between 10% and 50% in transgenic plants produced either by Agrobacterium-mediated transformation or through particle bombardment. Different effects such as deletion, duplication, rearrangement, repeated sequence recombination as well as gene interaction have been observed for transgenic loci. The nature of the recipient genome, nature of the transgene and the interactions between them seem to contribute to the non-Mendelian segregation of transgenes.
4

Cucumber transformation methods - the review

81%
Yin Z., Bartoszewski G., Szwacka M., Malepszy S.
Biotechnologia
|
2005
|
issue 1
95-113
EN
Several aspects of cucumber transformation including the ways of transgene introduction, factors influencing the transformation efficiency and the fate of the introduced genes were reviewed. Various transgenes have been introduced into the cucumber genome mostly via the Agrobacterium-mediated transformation. The frequency of Agrobacterium-mediated transformation ranged from 0.8 to 10% and was influenced by the selection agent, the regeneration efficiency, activation of vir genes expression, the explant size, bacteria cell density, the length of exposure and the co-cultivation period. The transgenes were integrated mostly as single copy in the Agrobacterium-mediated transformation and as multiple copies in direct transformation. Variable levels of the transgene expression were observed. The transmission of the transgenes as well as the transgenic phenotype follow the Mendelian, and rarely non-Mendelian, ratio. The production of marker-free transgenic cucumber and use of an alternative transformation method are recommended.
5
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Porcine insulin receptor substrate 2: molecular cloning, tissues distribution, and functions in hepatocyte and aortic endothelial cells

72%
Yin Z., Cai M., Weng X., Liu Z., Zhang G., Yin Z., Cai M., Weng X., Liu Z., Zhang G.
Polish Journal of Veterinary Sciences
|
2019
|
issue 3
6

Tissue-culture-responsive and autotetraploidy-responsive changes in metabolic profiles of cucumber (Cucumis sativus L.)

61%
Filipecki M., Yin Z., Wisniewska A., Smiech M., Malinowski R., Malepszy S.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 1
17-21
EN
Somaclonal variation commonly occurs during in vitro plant regeneration and may introduce unintended changes in numerous plant characters. In order to assess the range of tissue-culture-responsive changes on the biochemical level, the metabolic profiles of diploid and tetraploid cucumber R1 plants regenerated from leaf-derived callus were determined. Gas chromatography and mass spectrometry were used for monitoring of 48 metabolites and many significant changes were found in metabolic profiles of these plants as compared to a seed-derived control. Most of the changes were common to diploids and tetraploids and were effects of tissue culture. However, tetraploids showed quantitative changes in 14 metabolites, as compared to regenerated diploids. These changes include increases in serine, glucose-6P, fructose-6P, oleic acid and shikimic acid levels. Basing on this study we conclude that the variation in metabolic profiles does not correlate directly with the range of genome changes in tetraploids.
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