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1

RAPD-PCR analysis of various goose populations

100%
Maciuszonek A., Grajewski B., Bednarczyk M.
Folia Biologica
|
2005
|
vol. 53
|
issue 1-2
83-85
EN
The aim of this study was to genetically analyse by the RAPD-PCR method four indigenous Polish goose breeds, Kartuska (Ka), Lubelska (Lu), Kielecka (Ki) and Podkarpacka (Pd), in order to determine the band-sharing frequency as well as bands characteristic of the evaluated breeds. The birds were maintained as conservative flocks, accounting for a reserve of genetic resources. A total of 102 scorable bands were obtained, their number ranging from 0 to 8, depending on one of seven primers used and the group of birds analyzed, within a mean of 3.64. For each genetic group specific bands with given primers were obtained, suggesting their potential for use as population-specific markers, especially in ex-situ conservation methods. The results also suggest that keeping endangered geese as separate flocks is relevant for their preservation.
2

Expression of exogenous genes in blastodermal cells of chicken in vitro

76%
Bednarczyk M., Plucienniczak G., Plucienniczak A., Lakota P., Sochanik A., Dluzniewska A., Grajewski B.
Folia Biologica
|
2003
|
vol. 51
|
issue 3-4
189-194
EN
Chicken blastodermal cells (BCs) from stage X embryos produce both somatic and germline chimeras when injected into the subgerminal cavity of recipient embryos. Transfection of the donor cells in vitro could lead to the production of chimeras capable of transmitting the transgene to their offspring. The aim of this study was to transfer and express foreign genes under control of the ovalbumin promoter in the BCs. The results showed that luciferase activity in the BCs reached a plateau value with a 2.0:1.0 or 5.0:1.0 liposome ? DNA ratio and using 1mug of DNA. Under this same condition, no difference was found in relative activity between the pGL-control and pOVALUC plasmid. The expression of other exogenous genes (green fluorescent protein and interferon alpha2a) driven by the chicken ovalbumin promoter in cultured chicken blastodermal cells in vitro is possible by this assay. Hatchability of recipient embryos after injection of 1, 500 or 800 transfected BCs was compared. The advantage of using a smaller number (800) of injected transfected BCs was that early embryonic mortality was reduced and resulted in higher (P<0.01) hatchability (24.5%) than in the case of 1, 500 BCs injected.
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