Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 5

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Diversity of diploid androgenic Brussels sprout plants of R0 and R1 generations

100%
Kaminski P., Dyki B., Krzyzanowska D., Gorecka K.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 1
25-33
EN
Androgenic Brussels sprout plants were produced by the use of anther culture from the donor cultivar 'Philemon F1'. A total of 96 plants obtained from 20 androgenic R0 genotypes assigned as diploids were evaluated both in the generative and vegetative stage, in respect of their morphological characters: mean plant height; leaf size, colour and waxiness; leaf blade shape, blistering and attitude; number of sprouts; as well as their self-incompatibility and fertility. Androgenic R0 plants derived from each of the 20 embryos were highly diversified and differed from the donor in one or more morphological traits in the vegetative stage. Evaluated populations also varied in fertility and self-incompatibility. Six androgenic genotypes that set a sufficient amount of seeds of the R1 generation and 'Philemon F1' were evaluated in the field in respect of plant height, total and marketable yield per plot, shape of stem with sprouts, shape and density of sprouts, and spacing between sprouts. Only four diploid R0 and R1 populations may have some value for further breeding, as they are characterised by good vigour, high or medium ability for sprout generation, and sufficient fertility.
2

The influence of several factors on the efficiency of androgenesis in carrot

100%
Gorecka K., Krzyzanowska D., Gorecki R.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 3
265-269
EN
The influence of cultivar, donor plant and culture procedure on the efficiency of androgenesis was studied in carrot anther culture. Experiments were carried out on five carrot cultivars: CxC 9900 F1, Lucky B F1, HCM, Beta III and Perfekcja, which were chosen because of their high carotene contents. Two procedures of anther culture were compared: (1) incubation in darkness for two weeks, followed by exposure to continuous light and transfer onto a fresh medium of the same composition; and (2) incubation in darkness until embryos appeared, without transfer onto a fresh medium. Temperature was +27oC all the time. Genotype played an important role in the process of androgenesis in carrot anther culture.The efficiency was the highest in cv. HCM ? 5.6 embryos per 100 anthers. Considerable differences in the capacity for androgenesis were observed between individual donor plants. The ratio of embryos obtained per 100 anthers for cv. HCM varied from 0.0 to 48.9. The second procedure of anther culture proved to be more efficient, cheaper and less complicated.
3

Obtaining carrot (Daucus carota L.) plants in isolated microspore cultures

100%
Gorecka K., Kowalska U., Krzyzanowska D., Kiszczak W.
|
|
vol. 51
|
issue 2
141-147
EN
Microspores were cultured on the modified B5 liquid medium containing 2.4D (0.1 mg L?1), NAA (0.1 mg L?1), L-glutamine (500 mg L?1), L-serine (100 mg L?1), and sucrose (100 g L?1). The developmental stages of microspores and divisions were observed. Initially, the formation of binuclear and multicellular structures was noticed. Plants regenerated in the cultures in which the tetrad stage of microsporogenesis had predominated. Embryoids were still forming 24 weeks after the cultures were set up. Six weeks after the transfer of androgenetic embryos onto the B5 regeneration medium, they were converted into complete plants. Out of 90 androgenetic plants planted in a growth chamber, 42 plants adapted to the new conditions. All of those plants proved to be diploids in cytometric analysis.
4

Obtaining homozygous carrot plants with the aid of androgenesis in anther cultures

88%
Gorecka K., Krzyzanowska D., Kiszczak W., Kowalska U., Gorecki R.
Biotechnologia
|
2008
|
issue 2
142-152
EN
Experiments with anther cultures of 22 carrot cultivars were carried out to study the effect of various factors on the effectiveness of embryogenesis in these cultures. The factors included: the stage of microsporogenesis, genotype, training of donor plants and their growth conditions. A modified B5 medium (Gamborg, et al. 1968) containing 500 mg L-1 glutamine, 100 mg L-1 serine, 0.1 mg L-1 of 2,4D, 0.1 mg L-1 NAA, 100 g L-1 sucrose and 6.5 g L-1 agar were used to induce androgenesis. Regeneration was carried out on MS media and B5 with reduced concentration of sucrose at 20 g L-1 without aminoacids and hormones or with small amount of hormones. Substrates that were a mixture of various components, such as peat, sand, mineral wool and charcoal, were used for adaptation. Ploidy of the obtained plants was determined by cytometry method. Homozygosity of the plants was established using two isoenzymatic systems: PGI ? phosphoglucose isomerase, and AAT ? aspartate aminotransferase. Anatomical studies of embryogenesis during anther cultures were also carried out to confirm the androgenetic origin of embryos. It was found that the uninucleate stage was the most suitable time to stimulate microspores to produce embryos, and that bud length was a good external indicator of the stage of microsporogenesis. The studied cultivars differed in their ability to undergo androgenesis in vitro. It was shown that it was not necessary to remove all shoots and umbels except the main one. Generally, the embryos were obtained regardless of the way the donor plants were trained, even when the plants were not trained at all. The donor plants grown in a greenhouse produced more embryos than the plants grown in the field. On MS and B5 media without hormones, used to regenerate plants from embryos, secondary embryogenesis was found to take place followed by a conversion of embryos to complete plants, which subsequently resulted in better adaptation (more than 80% of plants became adapted). Cytometric studies revealed that more than 90% of the obtained androgenetic plants had a doubled chromosome complement. By analyzing the AAT and PGI isoenzymes, it was found that the obtained carrot androgenetic plants were homozygotes. Anatomical studies confirmed that embryos were formed from microspores.
5

Morphological diversity of androgenic carrot plants

76%
Kozik E. U., Nowak R., Klosinska U., Gorecka K., Krzyzanowska D., Gorecki R.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 1
49-53
EN
The aim of this study was to evaluate phenotypic variation of R0 androgenic plants obtained from four seed sources and donor plants by anther culture. Several morphological traits (leaf size, petiole length, leaf division, cortex colour) and the range of diversity were evaluated. There was large variation in all traits among the donor varieties. Especially leaf division and cortex colour differed significantly among the androgenic plants that came from different seed sources. The plants regenerated from four donor plants of variety 62 were significantly different in most traits except for leaf width and cortex colour. Evaluation of R1 plants will demonstrate whether the R0 variation observed is due to genetic variation or physiological differences from tissue culture.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.