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The organ transplantation ? a challenge for biotechnology

100%

Jasinski A., Slomski R., Szalata M., Lipinski D.

Biotechnologia

2006

issue 1

7-28

The use of animals as a source of organs and tissues for xenotransplantation can overcome the growing shortage of human organ donors. However, xenoreactive antibodies present in humans directed against swine Gal antigen on the surface of xenograft donor cells leads to the complement activation and immediate xenograft rejection as a consequence of hyperacute immunological reaction. The graft of genetically modified organ of a swine would be tolerated with simultaneous administration of medicines decreasing other less severe immunological reactions. This review summarizes the clinical history and rationale for xenotransplantation, recent progress in understanding the physiologic, immunologic, and infectious obstacles to xenotransplantation and some of the strategies being pursued to overcome these dificulties.

Visualisation of the extent of damage in a rat spinal cord injury model using MR Microscopy of the water diffusion tensor

76%

Krzyzak A. T., Jasinski A., Weglarz W. P., Adamek D., Sagnowski P., Baj M.

Acta Neurobiologiae Experimentalis

2005

vol. 65

issue 3

255-264

Magnetic Resonance Diffusion Tensor Imaging (DTI) of the control and traumatic injured spinal cord of a rat in vitro is reported. Experiments were performed on excised spinal cords from 10 Wistar rats, using a home-built 6.4 T MR microscope. MRI and histopathological results were compared. Presented results show that DTI of the spinal cord, perfused with formalin 10 minutes after the injury, can detect changes in water diffusion in white matter (WM) and in gray matter (GM), in areas extending well beyond the region of direct impact. Histology of neurons of the GM shows changes that can be attributed to ischemia. This is in agreement with the observed decrease of diffusion in the injured regions, which may be attributed to the cytotoxic edema due to ischemia. However, the diffusion changes in highly anisotropic WM seem to be caused by a direct action of mechanical force of impact, which significantly distorts the nerve fibers.

2-Deoxyglucose induces beta-APP overexpression, tau hyperphosphorylation and expansion of the trans-part of the Golgi complex in rat cerebral cortex

52%

Grieb P., Gordon-Krajcer W., Frontczak-Baniewicz M., Walski M., Ryba M. S., Kryczka T., Fiedorowicz M., Kulinowski P., Sulek Z., Majcher K., Jasinski A.

Acta Neurobiologiae Experimentalis

2004

vol. 64

issue 4

491-502

The effects of a single intraperitoneal injection of a non-metabolizable glucose analog 2-deoxyglucose (2-DG, 500 mg/kg) on the levels of b-APP expression, and phosphorylated and unphosphorylated tau protein in the rat cerebral cortex were investigated. The effects of 2-DG on the ultrastructure of cortical neurons with particular emphasis on the morphology of the Golgi apparatus, and on brain bioenergetics assessed by in vivo 31P-MRS technique were also evaluated. Seven and a half hours after injection of 2-deoxyglucose a significant increase in brain cortex b-APP expression, increased tau phosphorylation, and a marked relative expansion of the trans- part of the Golgi intracellular secretory pathway in cortical neurons has been found. The changes of b-APP expression and tau phosphorylation appeared within 1 h after 2-DG application and continued for at least 24 h. However, brain 31P resonance spectra remained unchanged for up to 7.5 h after 2-DG. It is suggested that the increase of b-APP expression represents a response of brain tissues to 2-DG-evoked biochemical stress, while tau hyperphosphorylation and the change in Golgi morphology may be secondary phenomena.

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2 Acta Neurobiologiae Experimentalis

1 Biotechnologia

1 2006

1 2005

1 2004

3 Jasinski A.

1 Adamek D.

1 Baj M.

1 Fiedorowicz M.

1 Frontczak-Baniewicz M.

1 Gordon-Krajcer W.

1 Grieb P.

1 Kryczka T.

1 Krzyzak A. T.

1 Kulinowski P.

1 Lipinski D.

1 Majcher K.

1 Ryba M. S.

1 Sagnowski P.

1 Slomski R.

1 Sulek Z.

1 Szalata M.

1 Walski M.

1 Weglarz W. P.

Search results

The organ transplantation ? a challenge for biotechnology

Visualisation of the extent of damage in a rat spinal cord injury model using MR Microscopy of the water diffusion tensor

2-Deoxyglucose induces beta-APP overexpression, tau hyperphosphorylation and expansion of the trans-part of the Golgi complex in rat cerebral cortex