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1

Effect of outer membrane proteins from Shigella humoral immunity induced in mice by SRBC

100%
Czarny A., Witkowska D., Mulczyk M.
Archivum Immunologiae et Therapiae Experimentalis
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1992
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vol. 40
|
issue 2
129-134
EN
Shigella flexneri outer membrane proteins (OMP) which had been earlier found to exert immunomodulatory effect on cell mediated immune response were also found to act as immunomodulator of the humoral immune response. Effects of OMP were investigated in the experiments in vitro and in vivo, where the level of humoral immune response measured as the number of plaque-forming cells (PFC) to SRBC in the spleen was evaluated. We demnostrate that small doses of OMP stimulate, whereas higher doses suppress the humoral immunity.
2

Fraction of spleen cells responsible for suppresion of cellular immune response to SRBC in mice treated with outer membrane proteins of Shigella

100%
Czarny A., Witkowska D., Mulczyk M.
Archivum Immunologiae et Therapiae Experimentalis
|
1992
|
vol. 40
|
issue 2
135-137
EN
Effect of splenocytes isolated from mice immunized with suppressive dose of OMP rfom Shigella on delaed hipersensivity, induced in mice with sheep red blood cells was investigated. Only the population of T lymphocytes was found to suppress the delayed hypersensitivity, as measured by the footpad reaction. The results suggest that OMP of Shigella are able to induce in the spleen of animals active T cells which are responsible for the suppression of cellular response induced by SRBC.
3

Some biological properties of outer membrane proteins of Hafnia

100%
Witkowska D., Czarny A., Mulczyk M.
Archivum Immunologiae et Therapiae Experimentalis
|
1992
|
vol. 40
|
issue 2
125-128
EN
Outer membrane proteins (OMP) isolated from four antigenically different strains of Hafnia alvei were tested for the toxicity, pyrogenicity, ability to induce Shwartzman reaction as well as for their influence on the leukocyte system. LD50 doses for the studied preparations determined on inbred mice were 18, 20, 28 and 34 mg/kg. These differences in the toxicity of the preparations were reflected in manifestation of Shwartzman reaction; more toxic preparations induced strongest necrohemorrhagic changes at the site of injection. The OMP preparations injected intravenously to rabbits caused moderate increase of body temperature. They induced changes in the number of leukocytes in the animals comparable with those of other preparates of bacterial origin.
4

Studies of immunity in mice immunized with outer membrane proteins of Hafnia

100%
Witkowska D., Czarny A., Mulczyk M.
Archivum Immunologiae et Therapiae Experimentalis
|
1992
|
vol. 40
|
issue 2
119-124
EN
Nonspecific protection induced in mice after administration outer membrane proteins of Hafnia alvei against infection with homologue and heterologous bacteria was transferred into other mice with lymphocytes isolated from spleens of mice immunized with outer membrane proteins. It was also found that mice sensitized with outer membrane proteins derived from H. alvei or with living bacteria induced in animals delayed hypersensitivity (DTH) in homologous and heterologous systems. The observed type of hypersensitivity was transferable to normal mice by lymphocytes obtained from donor animals which were previously sensitized with OMP. The experiments revealed that immunity induced with outer membrane proteins of Hafnia alvei is cell-mediated.
5
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Structural and serological characterization of Hafnia alvei lipopolysaccharide core region

64%
Ługowski C., Niedziela T., Jachymek W., Klonowska A., Czarny A., Rowiński S., Petersson C., Kenne L.
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