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SENSITIVITY TO CHITOSAN ASCORBATE MICROAEROPHILIC BACTERIA ISOLATED FROM INFECTIONS OF ORAL CAVITY

100%

Kochańska B., Kędzia A., Gębska A.

Progress on Chemistry and Application of Chitin and its Derivatives

2016

vol. 21

109-113

The aim of the date was to determine the sensitivity to chitosan ascorbate microaerophilic bacteria isolated from pathological pockets, root canals and apical abscesses. Chitosan was obtained from Antarctic krill. The experiments included 70 clinical isolates and 4 standards strains. The susceptibility of microaerophilic bacteria was performed by means of plate dilution technique in Brucella agar supplemented with 5% defibrinated sheep blood. Inoculum contained 105 CFU per spot. Incubation inoculated agar was performed at 37°C for 48 hrs in microaerophilic conditions. MIC was defined as the least concentrations of chitosan ascorbate that inhibited growth of tested bacteria. The results indicated that 47% of all date bacteria was susceptible to chitosan ascorbate in ranges ≤ 0.01 – 0.5 mg/ml. The most susceptible were the strains of Corynebacterium matruchotii (100%, MIC ≤ 0.01 – 0.5 mg/ml) and the least sensitive strains of Rothia dentocariosa (MIC in ranges 1.0 ˗ ≥ 4.0 mg/ml ).

EVALUATION ACTIVITIES OF CHITOSAN ASCORBATE AGAINST RODS OF HELICOBACTER PYLORI ISOLATED FROM GINGIVAL POCKETS AND ATHEROSCLEROTIC PLAQUES

88%

Kędzia A., Kochańska B., Gębska A., Wierzbowska M., Kufel A.

Progress on Chemistry and Application of Chitin and its Derivatives

2016

vol. 21

104-108

The aim of the study was determine activity of chitosan ascorbate against 17 strains of Helicobacter pylori. The strains were isolated from gingival pockets (7 strains), atherosclerotic plaques from carotid and femoral (10 strains). Chitosan was obtained from krill chitin and deacetylation degree was equal 60%. The ratio of ascorbic to chitosan was equal 1. The susceptibility of rods was determined by means of plate dilution technique in Brucella agar with 5% sheep’s blood. The inoculum contained 105 CFU/spot. Incubation was performed in anaerobic jars with Campy Pak (BBL) for 48 hrs. The MIC was interpreted as the lowest concentration of chitosan ascorbate inhibiting the growth of bacteria. The results indicated, that chitosan ascorbate at the lowest concentrations was active against 35% of the strains. The Helicobacter pylori rods isolated from gingival pockets were the highest susceptible than isolated from atherosclerotic plaques.

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2 Progress on Chemistry and Application of Chitin and its Derivatives

2 2016

2 Gębska A.

2 Kochańska B.

2 Kędzia A.

1 Kufel A.

1 Wierzbowska M.

Search results

SENSITIVITY TO CHITOSAN ASCORBATE MICROAEROPHILIC BACTERIA ISOLATED FROM INFECTIONS OF ORAL CAVITY

EVALUATION ACTIVITIES OF CHITOSAN ASCORBATE AGAINST RODS OF HELICOBACTER PYLORI ISOLATED FROM GINGIVAL POCKETS AND ATHEROSCLEROTIC PLAQUES