Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 7

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Characteristcs and regulation of the expression of genes coding Erwinia pectinases and its significance for soft rot disease

100%
Jafra S., Lojkowska E.
Biotechnologia
|
1999
|
issue 2
104-119
EN
The enterobacterium Erwinia causes soft rot disease in various plants. Erwinia pathogenicity results from the secretion of pectinolytic and cellulolytic enzymes responsible for the deterioration of the plant cell wall. It produces pectin methylesterases, several pectate lyases, polygalacturonase, pectin lyase, cellulases and hemicellulase. Regulation of pectinases genes requires several regulatory systems. Deletion of the major pectate lyase genes from Erwinia genome failed to totally eliminate tissue maceration. Structural genes for secondary pectate lyase were cloned and sequenced. The important role of the secondary pelL gene in the development of infection was demonstrated.
2

Expression of Erwinia chrysanthemi genes coding pectate lyases in infected potato tissue

100%
Jafra S., Lojkowska E.
Biotechnologia
|
2000
|
issue 3
202-214
EN
Erwinia chrysanthemi mutants, containing transcriptional fusion of one of the pectate lyase genes (pelA, pelB, pelC, pelD, pelE, pelI, pelL, pelZ) with the reporter gene encoding b-glucuronidase activity, were studied for their ability to cause disease symptoms and to synthesise pectinases after inoculation of potato tubers. The strains affected in pelI and pelL genes displayed a reduced virulence on potato tubers demonstrating the important role of these isoenzymes in soft rot disease. Analysis of the bacterial population showed an active multiplication of bacteria during the infection. Similar kinetics of growth were observed for all mutants and for the wild type strain. Comparison of the mutants and the wild type strain showed that the pelI, pelL and pelZ mutants synthesised reduced levels of Pels. The expression of pelA, pelE and pelZ is 5-fold higher in planta than in in vitro. In contrast, both pelI and pelL are highly (10-fold factor) induced in planta, which is characteristic of the plant-inducible pectate lyases.
3

Elicitation of secondary metabolites in vitro culture of Ammi visnaga

100%
Krolicka A., Lojkowska E.
Biotechnologia
|
2000
|
issue 4
112-117
EN
Ammi visnaga (Umbelliferae) are subtropical annual plants, which contain two groups of pharmaceutically important substances: furanochromones and piranocoumarins. In order to check the possibility of the production of secondary metabolites, in vitro cultures of callus and cell suspension were established. The study was concentrated on the induction of production of secondary metabolites by exposing callus and cell suspension cultures to abiotic elicitors: acetylsalicylic acid, jasmonic acid and suspension of silicon dioxide and biotic elicitors: autoclaved lysates of Enterobacter sakazaki, and scleroglucan. Thin layer chromatography of methanol extracts of cultures of A. visnaga did not indicate high induction of secondary metabolites production. Treatment of the callus cultures of A. visnaga with acetylsalicylic acid or jasmonic acid induce accumulation of furanochromone - visnagin and piranocoumarin ? samidin. Exposing the callus and cell suspension cultures to the suspension of silicon dioxide indicated an induction of accumulation of furanochromone - kelolglucoside. Further research will concentrate on quantitative determination of the level of accumulated compounds.
4

Resistance to antibiotics of the selected species of Agrobacterium rhizogenes

81%
Krolicka A., Kurlenda J., Lojkowska E.
Biotechnologia
|
1999
|
issue 2
94-103
EN
Agrobacterium rhizogenes is used for the transformation of plant cells and production of hairy roots cultures. In the presented work the bacteriostatic activity of several antibiotics on A. rhizogenes strains was tested. Different concentration of antibiotics belonging to the types of cefalosporin II and III generation, b-lactam and fluorochinolon were tested for elimination of the bacteria from transformed tissue. Out of all tested combinations, the mixture of carbenicilin and cefotaksym (claforan) was the most efficient for A. rhizogenes strains elimination from transformed plant tissues. The addition of those antibiotics to the regeneration medium was not toxic to plant tissues and it facilitated rapid growth of hairy roots.
5

Technology Luminex xMAP? ? a new tool in the diagnostics of plant diseases

81%
Sledz W., Los E., Lojkowska E.
Biotechnologia
|
2010
|
issue 1
97-108
EN
The presented paper reviews the aspects of Luminex xMAP? technology as a new tool in diagnostics of plant diseases. Luminex xMAP? technology, based on flow cytometry, employs tiny colored beads (microspheres) combined with specific analytes and ligands (antibodies, oligonulectydies ect.). Initially, this system was known as FlowMetrix. The Luminex xMAP? technology is mainly employed in medical diagnostics. This technology is suited to very wide range of medical applications: allergy testing, cancer markers, gene expression, genotyping, tissue typing and many others. Luminex xMAP? technology was applied in the diagnostics of plant diseases. The first attempts were directed towards the fungal and bacterial plant pathogens. Nowadays, this technology has been applied to detection of bacteria such as: Pectobacterium carotovorum or Dickeya dianthicola and viral pathogens such as: Potato Virus X, Potato Virus Y and Potato Leafroll Virus. The Luminex xMAP? technology offers simultaneous detection of several factors (pathogens) in one test.
6

Hairy root culture of Centaurium erythraea Rafn.

71%
Piatczak E., Wielanek M., Krolicka A., Lojkowska E., Wysokinska H.
Biotechnologia
|
2004
|
issue 2
29-36
EN
In this study, the transformed root culture of Centaurium erythraea was established, as a result of infection of leaves culture with agropine strain of Agrobacterium rhizogenes (LBA 9402). Frequency of root formation depended on the kind of explants and the presence of acetosyringone in the bacteria medium. The transformation was confirmed according to the analysis of opines and RAPD-PCR. As the result of transformation 30 clones of hairy roots were obtained. These clones have grown in liquid Woody Plant (WPM) medium without growth regulators. The curve of growth indicates that fresh weight of roots increased almost 17 times , and dry weight increased 12 times during 55 days. Hairy roots of C. erythraea were able to produce secoiridoid glycosides (sweroside, gentiopicroside, swertiamarine).
7

Cyanobacteria ? the source of biologically active substances

71%
Glowacka J., Waleron M., Szefel-Markowska M., Lojkowska E., Waleron K.
|
2007
|
issue 4
95-112
EN
Cyanobacteria (blue ? green algae) are one of the largest group of Gram ? negative, photosynthetic prokaryotes, which are morphologically diverse and highly widespread in salt and sweet water and also in terrestrial habitats. They play a significant role as primary producers in many ecosystems. Some species of Cyanobacteria are capable to grow extensively and formate toxic water blooms which can be dangerous for animal and human health. Certain species of them produce a wide variety of bioactive compounds which can have potential biotechnological cosmetical and pharmaceutical applications. Cyanobacteria have recently been identified as one of the most promising group of organisms from which large number of novel, biochemically active natural compounds can be isolated.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.