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In vitro co-stimulation of anti-tumor activity by soluble B7 molecules

100%

He W., Hu Z.-B., Liu F., Feng X.-Q., Zou P.

2006

vol. 53

issue 4

807-813

In order to investigate the anti-tumor activity of a soluble B7-1/immunoglobulin G fusion protein and explore an effective method to eliminate immune escape of tumor cells, a recombinant vector encoding this fusion protein was constructed and constitutively expressed in Chinese hamster ovary cells. After purification with protein G affinity chromatography, the soluble fusion protein was tested for bioactivity. Results showed that the fusion protein could significantly increase the density of B7-1 molecules on WEHI-3 cells, a mouse leukemia cell line. Through allogeneic mixed lymphocyte tumor cultures, it was demonstrated that, with the presence of the first signal, it could also significantly enhance T cell activation and killing activity against WEHI-3 cells and interleukin-2 secretion by activated mouse T lymphocytes. The conclusion can be drawn that the soluble B7-IgG fusion protein has a potent capacity to generate or enhance anti-tumor immune response in vitro, and its clinical value deserves further investigation.

Upregulation of GRP78 and caspase-12 in diastolic failing heart

86%

Sun Y., Liu G., Song T., Liu F., Kang W., Zhang Y., Ge Z.

2008

vol. 55

issue 3

511-516

Background: The endoplasmic reticulum (ER) fulfills multiple cellular functions. Various stimuli can potentially cause ER stress (ERS). ERS is one of the intrinsic apoptosis pathways and apoptosis plays a critical role in hypertension. Glucose regulated protein 78 (GRP78) has been widely used as a marker for ERS and caspase-12 mediated apoptosis was a specific apoptotic pathway of ER. The expression of GRP78 and caspase-12 remains poorly understood in the diastolic heart failure resulting from hypertension. Methods: We used spontaneously hypertensive rats (SHRs) to establish a model of diastolic heart failure, and performed immunohistochemistry, western blot, and real-time PCR to analyze GRP78 and caspase-12. Results: We found that GRP78 and caspase-12 had enhanced expression at protein and mRNA levels. Conclusions: These results suggest that GRP78 and caspase-12 were upregulated in cardiomyocytes and ERS can contribute to cardiac myocyte apoptosis in the diastolic heart failure resulting from hypertension.

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2 Acta Biochimica Polonica

1 2008

1 2006

2 Liu F.

1 Feng X.-Q.

1 Ge Z.

1 He W.

1 Hu Z.-B.

1 Kang W.

1 Liu G.

1 Song T.

1 Sun Y.

1 Zhang Y.

1 Zou P.

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In vitro co-stimulation of anti-tumor activity by soluble B7 molecules

Upregulation of GRP78 and caspase-12 in diastolic failing heart