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1
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Application of genetic semihomology algorithm to theoretical studies on various protein families.

100%
Leluk J., Hanus-Lorenz B., Sikorski A. F.
|
2001
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vol. 48
|
issue 1
21-33
EN
Several protein families of different nature were studied for genetic relationship, correct alignment at non-homologous fragments, optimal sequence consensus construction, and confirmation of their actual relevance. A comparison of the genetic semihomology approach with statistical approaches indicates a high accuracy and cognition significance of the former. This is particularly pronounced in the study of related proteins that show a low degree of homology. The sequence multiple alignments were verified and corrected with respect to the questionable, non-homologous fragments. The verified alignments were the basis for consensus sequence formation. The frequency of six-codon amino acids occurrence versus position variability was studied and their possible role in amino acid mutational exchange at variable positions is discussed.
2
Content available remote

Interaction of membrane skeletal proteins with membrane lipid domain.

100%
Sikorski A. F., Hanus-Lorenz B., Jezierski A., Dluzewski A. R.
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2000
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vol. 47
|
issue 3
565-578
EN
The object of this paper is to review briefly the studies on the interaction of red blood cell membrane skeletal proteins and their non-erythroid analogues with lipids in model systems as well as in natural membranes. An important question to be addressed is the physiological significance and possible regulatory molecular mechanisms in which these interactions are engaged.
3
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Optimization of flotation assay conditions for syndapin binding to phosphatidic acid containing liposomes

100%
Piaścik M., Zegarlińska J., Sikorski A. F., Czogalla A.
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
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2017
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vol. 13
9-17
PL
Flotacja jest jedną z najefektywniejszych metod wstępnej identyfikacji oddziaływań białko-błony lipidowe. W większości przypadków wykorzystuje się w niej małe jednowarstwowe pęcherzyki lipidowe, które służą jako modele błonowe i nie wymagają dodatkowych nośników, takich jak membrany czy nanocząstki polimerowe, które są często używane w innych metodach mających na celu identyfikację oddziaływań białkolipid. W poniższej pracy prezentujemy wyniki uzyskane podczas badań oddziaływań kwasu fosfatydowego i syndapiny. Omawiamy także niektóre techniczne aspekty metody, kładąc nacisk na to jak małe zmiany w warunkach metody mogą wpłynąć na otrzymywane wyniki.
EN
Flotation is one of the best method for preliminary identification of protein-lipid interactions. In most widely used approach it utilizes large unilamellar vesicles, that are excellent models of freestanding membranes and do not require any additional components, like solid supports or beads that are needed in other methods commonly used for protein-lipid binding studies. Here we present results obtained during our studies on phosphatidic acid - syndapin interactions and discuss some technical aspects of this method underlying how relatively small changes in the conditions can influence the results.
4
Content available remote

Attaching a spin to a protein - site-directed spin labeling in structural biology

100%
Czogalla A., Pieciul A., Jezierski A., Sikorski A. F.
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2007
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vol. 54
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issue 2
235-244
EN
Site-directed spin labeling and electron paramagnetic resonance spectroscopy have recently experienced an outburst of multiple applications in protein science. Numerous interesting strategies have been introduced for determining the structure of proteins and its conformational changes at the level of the backbone fold. Moreover, considerable technical development in the field makes the technique an attractive approach for the study of structure and dynamics of membrane proteins and large biological complexes at physiological conditions. This review focuses on a brief description of site-directed spin labeling-derived techniques in the context of their recent applications.
5
Content available remote

Ankyrin shares a binding site with phospholipid vesicles on erythrocyte spectrin

100%
Białkowska K., Zembroń A., Sikorski A. F.
Acta Biochimica Polonica
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1994
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vol. 41
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issue 2
155-157
6
Content available remote

Proteolysis of spectrin by trypsin and pronase in the presence of phospholipid suspensions

100%
Sikorski A. F., Kozubek A., Szopa J.
Acta Biochimica Polonica
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1988
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vol. 35
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issue 2
71-81
7
Content available remote

Expression, purification and functional characterization of recombinant human acyl-CoA-binding protein (ACBP) from erythroid cells

76%
Augoff K., Kolondra A., Chorzalska A., Lach A., Grabowski K., Sikorski A. F.
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2010
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vol. 57
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issue 4
533-540
EN
Fatty acyl-CoA esters are extremely important in cellular homeostasis. They are intermediates in both lipid metabolism and post-translational protein modifications. Among these modification events, protein palmitoylation seems to be unique by its reversibility which allows dynamic regulation of the protein hydrophobicity. The recent discovery of an enzyme family that catalyze protein palmitoylation has increased the understanding of the enzymology of the covalent attachment of fatty acids to proteins. Despite that, the molecular mechanism of supplying acyl-CoA esters to this reaction is yet to be established. Acyl-coenzyme A-binding proteins are known to bind long-chain acyl-CoA esters with very high affinity. Therefore, they play a significant role in intracellular acyl-CoA transport and pool formation. The purpose of this work is to explore the potential of one of the acyl-CoA-binding proteins to participate in the protein palmitoylation. In this study, a recombinant form of ACBP derived from human erythroid cells was expressed in E. coli, purified, and functionally characterized. We demonstrate that recombinant hACBP effectively binds palmitoyl-CoA in vitro, undergoing a shift from a monomeric to a dimeric state, and that this ligand-binding ability is involved in erythrocytic membrane phosphatidylcholine (PC) remodeling but not in protein acylation.
8
Content available remote

Brain spectrin interacts with membrane phospholipids

74%
Diakowski W., Sikorski A. F.
Acta Biochimica Polonica
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1994
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vol. 41
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issue 2
153-154
9
Content available remote

Interaction of spectrin with hydrophobic agaroses

74%
Sikorski A. F.
Acta Biochimica Polonica
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1988
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vol. 35
|
issue 1
19-27
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