Search results

Sort By:

Limit search:

Invasive Cx43high sub-line of human prostate DU145 cells displays increased nanomechanical deformability

100%

Piwowarczyk K., Sarna M., Ryszawy D., Czyż J.

2017

vol. 64

issue 3

445-449

Connexin(Cx)43high cells are preferentially recruited to the invasive front of prostate cancer in vitro and in vivo. To address the involvement of Cx43 in the regulation of human prostate cancer DU145 cell invasiveness, we have analysed the nanoelasticity of invasive Cx43high sub-sets of DU145 cells by atomic force microscopy (AFM). The Cx43high DU145 cells displayed considerably higher susceptibility to mechanical distortions than the wild type DU145 cells. Transient Cx43 silencing had no effect on their elastic properties. Our data confirm the relationship between the invasive potential, Cx43 expression and nanoelasticity of the DU145 cells. However, they also show that Cx43 is not directly involved in the maintenance of DU145 invasive phenotype.

Connexin-dependent intercellular stress signaling in tissue homeostasis and tumor development

64%

Czyż J., Piwowarczyk K., Paw M., Luty M., Wróbel T., Catapano J., Madeja Z., Ryszawy D.

2017

vol. 64

issue 3

377-389

Cellular stress responses determine tissue development, homeostasis and pathogenesis. Paracrine signaling, exchange of mechanical stimuli and intercellular transfer of small metabolites via connexin-built gap junctional channels are involved in the cellular stress detection and propagation of stress stimuli in multicellular networks. Cellular stress responses are also regulated through the activity of unpaired connexons (hemichannels) and via the intracellular interference of connexins with the cell cycle and pro-apoptotic machinery. Therefore, connexins are considered as multidirectional transmitters of the "outside-in" and "inside-out" stress signaling that are crucial for tissue homeostasis, regeneration and pathology. In particular, the disturbance of connexin function during the multi-stage process of tumor development leads to abnormal reactions of tumor cells to stress stimuli. In this review, we outline the current knowledge on the multidirectional role of connexins in the detection of stress signals. We also discuss the role of connexin-mediated intercellular transmittance of stress signals in tumour promotion, progression and metastatic cascade. Highlights: 1. Connexins and gap junctions protect cells from the microenvironmental stress and are involved in propagation and intracellular processing of stress signals. 2. The quality and quantity of stress stimuli, which may lead to cell adaptation or death by apoptosis, is determined by intrinsic properties of connexins and the cell phenotype. 3. Connexin deficiency increases the resistance of tumor cells to the "outside-in" stress signaling. 4. The connexin-mediated "inside-out" stress signaling participates in tumor cell invasion during the metastatic cascade.

Refine search results

2 Acta Biochimica Polonica

2 2017

2 Czyż J.

2 Piwowarczyk K.

2 Ryszawy D.

1 Catapano J.

1 Luty M.

1 Madeja Z.

1 Paw M.

1 Sarna M.

1 Wróbel T.

Search results

Invasive Cx43high sub-line of human prostate DU145 cells displays increased nanomechanical deformability

Connexin-dependent intercellular stress signaling in tissue homeostasis and tumor development