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Effect of antisense peptide binding on the dimerization of human cystatin C - gel electrophoresis and molecular modeling studies.

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Stachowiak K., Rodziewicz-Motowidło S., Sosnowska R., Kasprzykowski F., Łankiewicz L., Grubb A., Grzonka Z.

vol. 51

issue 1

153-160

Human cystatin C (HCC) shows a tendency to dimerize. This process is particularly easy in the case of the L68Q HCC mutant and might lead to formation of amyloid deposits in brain arteries of young adults. Our purpose was to find ligands of monomeric HCC that can prevent its dimerization. Eleven antisense peptide ligands of monomeric HCC were designed and synthesized. The influence of these ligands on HCC dimerization was studied using gel electrophoresis and molecular modeling methods. The results suggest that all the designed peptides interact with monomeric HCC facilitating its dimerization rather than preventing it.

Governing the monomer-dimer ratio of human cystatin c by single amino acid substitution in the hinge region

100%

Szymańska A., Radulska A., Czaplewska P., Grubb A., Grzonka Z., Rodziewicz-Motowidło S.

issue 3

455-463

Three dimensional domain swapping is one of the mechanisms involved in formation of insoluble aggregates of some amyloidogenic proteins. It has been proposed that proteins able to swap domains may share some common structural elements like conformationally constrained flexible turns/loops. We studied the role of loop L1 in the dimerization of human cystatin C using mutational analysis. Introduction of turn-favoring residues such as Asp or Asn into the loop sequence (in position 57) leads to a significant reduction of the dimer fraction in comparison with the wild type protein. On the other hand, introduction of a proline residue in position 57 leads to efficient dimer formation. Our results confirm the important role of the loop L1 in the dimerization process of human cystatin C and show that this process can be to some extent governed by single amino acid substitution.

Differential effects of various soy isoflavone dietary supplements (nutraceuticals) on bacterial growth and human fibroblast viability

100%

Pierzynowska K., Rzeszótko A., Blendowska A., Wieczerzak E., Rodziewicz-Motowidło S., Piotrowska E., Węgrzyn G.

2018

vol. 65

issue 2

325-332

Flavonoids, polyphenolic compounds present in many food products, affect growth of different bacterial species when tested as purified or synthetic substances. They can also influence gene expression in human cells, like fibroblasts. Here, we asked if soy isoflavone extracts, commonly used in many products sold as anti-menopausal dietary supplements, influence bacterial growth similarly to a synthetic isoflavone, genistein. Four commercially available products were tested in amounts corresponding to genistein concentrations causing inhibition of growth of Vibrio harveyi (a model bacterium sensitive to this isoflavone) and Escherichia coli (a model bacterium resistant to genistein). Differential effects of various extracts on V. harveyi and E. coli growth, from stimulation, to no changes, to inhibition, were observed. Moreover, contrary to genistein, the tested extracts caused a decrease (to different extent) in viability of human dermal fibroblasts. These results indicate that effects of various soy isoflavone extracts on bacterial growth and viability of human cells are different, despite similar declared composition of the commercially available products.

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3 Acta Biochimica Polonica

1 2018

1 2009

1 2004

3 Rodziewicz-Motowidło S.

2 Grubb A.

2 Grzonka Z.

1 Blendowska A.

1 Czaplewska P.

1 Kasprzykowski F.

1 Pierzynowska K.

1 Piotrowska E.

1 Radulska A.

1 Rzeszótko A.

1 Sosnowska R.

1 Stachowiak K.

1 Szymańska A.

1 Wieczerzak E.

1 Węgrzyn G.

1 Łankiewicz L.

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Effect of antisense peptide binding on the dimerization of human cystatin C - gel electrophoresis and molecular modeling studies.

Governing the monomer-dimer ratio of human cystatin c by single amino acid substitution in the hinge region

Differential effects of various soy isoflavone dietary supplements (nutraceuticals) on bacterial growth and human fibroblast viability