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Ellipsometrical and SEM Study of Ferritin Adsorption οn a Gold Surface

100%
Vladimirova L., Tsutsumanova G., Gritzkow A., Savov V.
Acta Physica Polonica A
|
2009
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vol. 116
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issue 4
705-707
EN
The adsorption of horse spleen ferritin on gold surface is studied with null ellipsometry and scanning electron microscopy. The high sensitivity and in situ advantage of ellipsometry make it very attractive for studies of ultrathin biomolecular layers. The influence of pH and protein concentration in solution on the adsorption kinetics was interpreted using ex situ scanning electron microscopy shots and computer modeling of the system "prism-gold film-adsorbed layer-solution". It seems that the first fast stage comprises nucleation process occurring at active sites and the following slow multistage process of a protein film growth. The data about adsorption kinetics of protein and the structure of the adsorbate layers obtained in this study enable understanding of such processes in real biological system, e.g. the protein adsorption on blood vessels and tissues in pathological processes.
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An Ellipsometrical and AFM Study οf Ferritin Adsorption Kinetics on a Gold Surface

88%
Vladimirova L., Andreeva A., Savov V., Gritzkow A., Burova M.
Acta Physica Polonica A
|
2009
|
vol. 116
|
issue 4
687-689
EN
The kinetics of ferritin adsorption on gold (Au) surface was studied with ellipsometry and atomic force microscopy. The thickness and the refraction index of the adsorbed layer were determined using a theoretical model system: air-interface layer-solution-adsorbed layer-gold surface. The relations between the parameters of the adsorbed layer and pH and the protein concentration are obtained. It seems that the ferritin adsorption is a relatively slow multi-stage process. The obtained results enable modelling of the protein adsorption on blood vessels and tissues in pathological processes. The kinetics of protein adsorption could be implemented in medical applications and in development of new biosensor systems.
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