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Application of Response Surface Methodology for Optimization of Permeabilization Process of Baker’s Yeast

100%
Trawczyńska I., Wójcik M.
Polish Journal of Chemical Technology
|
2014
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vol. 16
|
issue 2
31-35
EN
Permeabilization was used for the purpose of transforming the cells of microorganisms into biocatalysts with an enhanced enzyme activity. Baker’s yeast cells were permeabilized with various organic solvents. A high degree of catalase activity was observed upon permeabilization with acetone, chloroform, isopropyl alcohol and ethyl acetate. Response surface methodology was used to model the effect of concentration of isopropyl alcohol, temperature and treatment time on the permeabilization of baker’s yeast cells to maximize the decomposition of H2O2. The optimum operating conditions for permeabilization were observed at 53.7% concentration of isopropyl alcohol, treatment time of 40 min and temperature of 15.6oC. A maximum value of catalase activity was found to be 6.188 U/g wet wt. and was ca. 60 times higher than the catalytic activity of yeast not treated by the permeabilization process.
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The effect of pH on the sorption of copper ions by alginates

81%
Kwiatkowska-Marks S., Miłek J., Wójcik M.
Polish Journal of Chemical Technology
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2008
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vol. 10
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issue 1
28-30
EN
The effect of pH on copper ions sorption by alginates has been investigated. The granules of the sorbent obtained from sodium alginate (type DMF, manufactured by KELCO) have been applied. By using a new method of gelation a biosorbent containing from 11.3 to 20.3% mas. of alginate has been produced. Investigations have been carried out at a constant temperature of 25°C and the pH values ranging from 3 to 5. The obtained experimental results have been described by the Langmuir equation. It has been found that with the increasing pH of the solution the maximum biosorbent sorption capacity increased.
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An alternative method to determine the diffusion coefficient for the shrinking core model

81%
Kwiatkowska-Marks S., Wójcik M., Kopiński L.
Polish Journal of Chemical Technology
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2011
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vol. 13
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issue 2
54-56
EN
A new method to determine the effective diffusion coefficient of sorbate in sorbent granule based on the analytical solution of the shrinking core model (SCM) has been proposed. The experimental data presented by Lewandowski and Roe1 concerning the sorption of copper ions by alginate granules have been applied to compare the analytical and numerical methods. The results obtained by both methods are very close.
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