The fatty acid composition of oil of the zero erucic acid commercial Brassica napus L. is typical for this species. It is rich in oleic acid and contains moderate levels of linoleic and linolenic acid. For human nutrition, it is advantageous primarily to obtain the highest possible content of oleic acid and to maintain the 2:1 ratio of linoleic to linolenic acid, while preserving the average total content of saturated acids. Uni- and multivariate analyses of variance were used for evaluation of doubled haploid lines of winter oilseed rape in respect of five fatty acids: palmitic (C16:0), stearic (C18:0) oleic (C18:1), linoleic (C18:2) and linolenic (C18:3). Some proposals of studying doubled haploid (DH) lines with the use of canonical transformation were also given. In MANOVA, the five original variables (individual fatty acids) were replaced by three 'new' variables (combinations of these acids) and used to evaluate DH lines with respect to the equirements concerning the nutritional role of fatty acids. The first variable was the total content of the saturated acids (C16:0 + C18:0), the second (unchanging) was the content of the monounsaturated acid C18:1, and the third was the difference between polyunsaturated acids, i.e. between linoleic acid, and the doubled content of linolenic acid (C18:2 ? 2 ? C18:3).
Many species of Cuphea genus show promise as rich natural sources of valuable seed oils for commercial utility.Among them are C.lancelota and C.viscosissima which seed oils contain mediumm-chain fatty acids.Recoprocal crosses were made between species to assess their usefulnes for Cuphea breeding programme.The degree of crossability between C.lanceolata and C.viscosissima was determinated by pollen grain germinatin and pollen tube growth observed using fluorescence microscopy in addition to capsule formation and seed number per capsule.Treatment of the pollen grain with a laser microbeam reduced the number of germinated pollen grains, tubes growth in the pistil and seed set when C viscisissima plants were used in the crosses as a female form.In the cross C.lanceolata (female form) x C viscosissima the pollen treatment with a laser microbeam improved pollen tube growth but reduced seed set.Crossability evaluated on the basis of pollen tube growth and seed set showed unilateral intraspecific incompatibility in crosses involving C.lanceolata plants as a female form.
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