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Ligands of peroxisome proliferator-activated receptor-γ increase the generation of vascular endothelial growth factor in vascular smooth muscle cells and in macrophages.

100%

Jozkowicz A., Dulak J., Piatkowska E., Placha W., Dembinska-Kiec A.

2000

vol. 47

issue 4

1147-1157

Peroxisome proliferator-activated receptors-γ (PPARγ) are ligand-inducible transcription factors of the nuclear hormone receptor superfamily. We examined the effect of PPARγ activation on the generation of vascular endothelial growth factor (VEGF), one of the major angiogenic agents. Rat vascular smooth muscle cells (VSMC) and murine macrophages RAW264.7 were incubated for 24 h with PPARγ activators: prostaglandin J2 and ciglitazone. PPARγ were expressed in VSMC and RAW cells and their activity was upregulated in the presence of PGJ2 and ciglitazone. Incubation of the cells with PPARγ activators significantly augmented the release of VEGF protein into the media, both in resting and in IL-1β- or LPS-stimulated cultures. The higher protein generation was connected with the increased expression of mRNA and transcriptional activation of VEGF promoter. We conclude that the activation of PPARγ upregulates the generation of VEGF and may be involved in the regulation of angiogenesis.

Relation of the protein glycation, oxidation and nitration to the osteocalcin level in obese subjects

59%

Razny U., Goralska J., Zdzienicka A., Fedak D., Masania J., Rabbani N., Thornalley P., Pawlica-Gosiewska D., Gawlik K., Dembinska-Kiec A., Solnica B., Malczewska-Malec M.

2017

vol. 64

issue 3

415-422

Carboxylated osteocalcin (Gla-OC) contributes to the bone formation, whereas its undercarboxylated form (Glu-OC) takes part in the energy metabolism. In vitro studies had shown that treatment of osteoblast-like cells with advanced glycation end product-modified bovine serum resulted in reduced synthesis of collagen 1 and osteocalcin. The aim of this study was to find association between Gla-OC and markers of protein glycation, oxidation and nitration, as well as pro-inflammatory and antioxidant defense markers in obese subjects. Non-obese [(body mass index (BMI)<30 kg/m; n=34)] and obese subjects (30

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2 Acta Biochimica Polonica

1 2017

1 2000

2 Dembinska-Kiec A.

1 Dulak J.

1 Fedak D.

1 Gawlik K.

1 Goralska J.

1 Jozkowicz A.

1 Malczewska-Malec M.

1 Masania J.

1 Pawlica-Gosiewska D.

1 Piatkowska E.

1 Placha W.

1 Rabbani N.

1 Razny U.

1 Solnica B.

1 Thornalley P.

1 Zdzienicka A.

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Ligands of peroxisome proliferator-activated receptor-γ increase the generation of vascular endothelial growth factor in vascular smooth muscle cells and in macrophages.

Relation of the protein glycation, oxidation and nitration to the osteocalcin level in obese subjects