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The Silver Colloidal Solutions and Films for Efficient SERS

100%
Górska A., Meyer S., Balter A.
Acta Physica Polonica A
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2012
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vol. 122
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issue 2
350-352
EN
The silver colloids and silver films are known for their signal enhancing properties in Raman spectroscopy. Surface enhanced raman spectroscopy is a very useful tool to detect molecules at low concentration or even single molecules in examined samples. Here we present a study on the preparation of silver colloidal solution and deposition of silver films on mica. The colloidal solutions were examined by two methods: the UV-VIS spectroscopy to determine their optical properties and atomic force microscopy to determine the size of the particles and structure of the deposited films. The crystal violet (tris(4-(dimethylamino)phenyl)methylium chloride) solution was used as a test sample for evaluation of enhancing properties of silver colloidal solutions and films. These experiments have shown that both colloidal solutions and films, are efficient surface enhanced raman spectroscopy substrates, therefore they can be used in further studies for enhancement of the Raman signal of biopolymers, f.e. polysaccharides and DNA.
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Influence of Silver Colloid on Fluorescein Spectral Properties

100%
Frąckowiak D., Balter A., Karolczak J., Siejak P.
Acta Physica Polonica A
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2006
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vol. 110
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issue 4
451-458
EN
In order to check if the observed earlier changes in fluorescence intensities of fluorescein solution due to the addition of colloidal suspension of silver are related to the generation of surface plasmon coupled emission, the steady state photothermal spectra and fluorescence decay times of such samples were measured. The Gaussian components of photoacoustic spectra for solutions with and without silver colloid addition were determined and compared with components of absorption and fluorescence excitation spectra of the same samples. The efficiencies of thermal and radiative deactivation of various samples were discussed. The fluorescence decay times measured at the wavelength of excitation not exhibiting the increase in photonic mode density depend only on the dye concentration. Decays exhibit changes as a result of silver addition with excitation in the region in which enhancement of fluorescence is observed. These changes are due not only to the change in the fluorescence yield generated by interactions with plasmons but also to those in thermal deactivation of the sample related to the colloid presence.
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AFM Investigation of Biological Nanostructures

76%
Strzelecki J., Dąbrowski M., Strzelecka J., Tszydel M., Mikulska K., Nowak W., Balter A.
Acta Physica Polonica A
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2012
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vol. 122
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issue 2
329-332
EN
Nanostructures created by living organisms, optimized through millions of years of evolution, can be a valuable inspiration for nanotechnology. We employ atomic force microscopy to examine such structures in materials created by common organisms - caddisfly and diatoms. Caddisfly larvae are well known for their ability to spin silk, which serves as an "adhesive tape" to glue various materials and collect food in aqueous environment. Atomic force microscopy imaging of caddisfly silk, performed for the first time by our team, has shown that its surface is patterned with 150 nm extensions - a feature related to its exceptional underwater sticking abilities. Results of force spectroscopy of protein structures found on the surface are also shown. A characteristic feature of diatoms is that they are encased within a unique silica cell wall called frustules, patterned with 200 nm pores, which allow cellular interaction with the environment. We perform atomic force microscopy imaging of frustules in living diatoms as well as adhesion measurements inside pores.
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