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1

STUDIES ON THE USABLE PROPERTIES OF INNOVATIVE WOUND DRESSINGS

100%

Kucharska M., Struszczyk M. H., Cichecka M., Brzoza K.

Progress on Chemistry and Application of Chitin and its Derivatives

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2012

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vol. 17

133 - 140

EN

The new implemented wound dressings should meet several requirements connected with the clinical safety and performance. The series of PN-EN 13726 Standards describes the tests methods for the verification the most important parameters related to mentioned performance and safety of a new designed wound dressing being the base for the risk analysis (according to PN-EN ISO 14971:2009 Standard). The aim of the research was to evaluate the behavior of several types of new designed prototypes of innovative wound dressings made of various form of chitosan (such as: foams, films, etc.). The behavior of studied chitosan wound dressings was evaluated before and after of accelerated aging according to ASTM F 1980-07:2002 to establish the effect of the wound dressings storing conditions. The presented study is a continuation of earlier research.

2

PRELIMINARY STUDIES ON THE USABLE PROPERTIES OF INNOVATIVE WOUND DRESSINGS

100%

Kucharska M., Struszczyk M. H., Cichecka M., Brzoza-Malczewska K.

Progress on Chemistry and Application of Chitin and its Derivatives

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2011

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vol. 16

131 - 138

EN

There is endless and growing demand on a novel and innovative wound dressings, which except basic protective performance accelerate the process of wounds healing and/or replace the patient skin function. The studies on the new wound dressings result from the need of the quick and effective treatment of wounds, such as: having no tendency to heal, posttraumatic wounds, scalding wounds, ulcerations or bedsores. Chitosan is a biopolymer originated from chitin by N-acetylation. Due to the special behavior, such as: biological activity, it is linked to polymers having several and multifunctional applications, especially for designing of medical devices. The aim of the research was to evaluate the comprehensive performance of several kinds of chitosan prototypes of innovative wound dressings made of various usable form of chitosan. The evaluation of mentioned prototypes of wound dressing was carried out using the guidelines from series of PN-EN 13726 Standards harmonized with UE Directive 93/42/EEC and 2007/47/WE for medical devices.

3

PREPARATION OF MICRO AND NANOSTRUCTURES OF CHITOSAN BY ULTRASONIC COALESCENCE OF W/O EMULSIONS

100%

Balcerzak J., Kucharska M., Gruchała B.

Progress on Chemistry and Application of Chitin and its Derivatives

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2013

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vol. 18

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issue 18

13-20

EN

In the paper, the preeliminary study of chitosan micro and nanospheres precipitation due to two reverse emulsions (W/O) coalescence is described. The composition conditions, which must be filled to obtain stable reverse emulsion is pointed out. Furthermore, the parameters of ultrasonic emulsification and coalescence technique are presented and finally, morphological study of obtained micro and nanospheres are discussed.

4

POLYMER BIOCOMPOSITES USED IN BEDSORES TREATMENT

88%

Wiśniewska-Wrona M., Kucharska M., Kardas I., Bodek A., Bodek H.

Progress on Chemistry and Application of Chitin and its Derivatives

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2011

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vol. 16

111 - 120

EN

Investigations are presented in the preparation of composite dressing material based on two biopolymers -chitosan and sodium alginate with the addition of sulfanilamide as medication designed for the healing of bedsores. The dressing was prepared in the form of film. The biopolymers used in the construction of the film make the dressing biodegradable and resorbable in the wound’s environment. Mechanical properties of the film were tested: thickness, extension strength, tenacity and elongation at maximum stress The ability of the material to match the wound was examined, too, as well as the transmission of water vapor. Sulfanilamide as bacteriostatic agent was added to the prepared composites. Mechanical and sorption properties of the composite dressings with addition of the active substance depend largely on their composition. The sorption properties were tested before and after addition of the medication .The release of the medication is intricate and proceeds according to kinetics of first order. Susceptibility of the composite materials to hydrolytic and enzymatic degradation was assessed.

5

THE ROLE OF OLIGOCHITOSANS IN AKT KINASE REGULATION

88%

Ignacak J., Wiśniewska-Wrona M., Dulińska-Litewka J., Pałka I., Kucharska M.

Progress on Chemistry and Application of Chitin and its Derivatives

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2015

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vol. 20

73-81

EN

Among characteristic properties of cancers, there is their increased glycolytic activity.Contrary to normal cells, neoplastic cells use anaerobic glycolysis, even when a sufficient amount of oxygen is available. The intensity of the process is associated with a considerable demand for energy in the form of ATP. Akt, which - acting through the mTOR pathway - activates the HIF-1 factor, which in turn activates hexokinase that participates in glucose phosphorylation, stimulates the transport of glucose to cells via increasing glucose transporters (GLUT) and activates lactate dehydrogenase (which transforms pyruvate to lactate). Chitosan, as well as products of its degradation - oligochitosans - contribute to inhibiting the activity of the Akt kinase, and thus contribute to inhibiting excessive glycolytic activity of Ehrlich ascites tumor (EAT) cells and to decreasing proliferation of these cells.

6

POTENTIAL USE OF CHITOSAN – BASED MATERIALE IN MEDICINE

88%

Kucharska M., Ciechańska D., Niekraszewicz A., Wiśniewska-Wrona M., Kardas I.

Progress on Chemistry and Application of Chitin and its Derivatives

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2010

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vol. 15

169 - 176

EN

Polysaccharides are macromolecular polymers that manifest ability toward the forming of fibres, film and coatings. Some of their specific properties like biodegradability and biocompatibility make them suitable for medical application. Chitin, chitosan and alginates are basic polymers mostly used in the preparation of medical biomaterials. In the Institute of Biopolymers and Chemical Fibres (IBWCh) multidirectional investigations are in full swing concerned with the use medical of polysaccharides and their various useful forms. The research includes amongst: multifunctional dressing materials, implants and other polysaccharide biomaterials.

7

INHIBITION OF EHRLICH ASCITES TUMOUR (EAT) CELLS PROLIFERATION THROUGH CHITOSAN-MEDIATED REGULATION OF ACTIVITY OF THE AKT PATHWAY

88%

Ignacak J., Wiśniewska-Wrona M., Dulińska-Litewka J., Pałka I., Kucharska M.

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EN

Isoenzyme M2 pyruvate kinase, which is a marker of cancer transformation, can take both tetramer (cytosol) and dimer (nucleus) forms. The former is responsible for ATP synthesis, and the latter demonstrates histone H1 kinase activity. Regulation of the expression of pyruvate kinase through which Akt controls the expression of genes involved in Ehrlich ascites tumour (EAT) cell proliferation, migration and death, also involves cross-talk with the other signalling pathways, transcription factors and co-regulatory proteins such as β-catenin and c-Myc. Treatment of EAT cells with chitosans significantly reduced their proliferation (by 45-60%), expression of nuclear β-catenin, c-Myc as well as cell migration. After 48–72 hours of treatment of the cell with oligochitosans, lower levels of p-Akt were detected. Simultaneously, decreased expression of isoenzyme M2 PK protein levels was observed. The dimeric form (nucleus) can participate in H1 histone phosphorylation, which contributes to increased EAT cell proliferation.

8

ADVANCED CELLULOSIC MATERIALS

76%

Brzoza-Malczewska K., Kucharska M., Wiśniewska-Wrona M., Guzińska K., Jóźwicka J., Bacciarelli-Ulacha A.

Progress on Chemistry and Application of Chitin and its Derivatives

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2015

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vol. 20

7-17

EN

Aim of the work was to prepare a method of producing chitosan and chitosan-alginate nanoparticles designed for the modification of textile cellulosic products in hygiene and medical application. Spectrophotometry was used in the estimation of the prepared nanoparticles; analyzed, too, was the particle size and antibacterial and antifungal activity.

9

THE ROLE OF CHITOSAN IN AKT KINASE REGULATION ACTIVITY

76%

Ignacak J., Wiśniewska-Wrona M., Dulińska-Litewka J., Pałka I., Kucharska M., Kazimierski J.

Progress on Chemistry and Application of Chitin and its Derivatives

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2016

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vol. 21

73-82

EN

A decrease in migration of tumor cells incubated with the investigated chitosan preparations was correlated with a decreased activity of MMP-2 and MMP-9 metalloproteinases, what significantly affected inhibition of tumor cell proliferation. In the investigations of the effects of various chitosan preparations on expression of PCNA, Akt and β-catenin in the normal human 184A1 cells and in breast carcinoma MCF7 cells evaluated at the protein level, significant differences in inhibition of expression of selected genes were noted in the tumor cells. Similarly as in the case of human cells, in mouse cells, the differences in expression of the investigated genes involved solely the Ehrlich carcinoma cells. In the presence of the investigated chitosan preparations, there was observed inhibition of expression of the N-cadherin, β-catenin, Akt and PCNA genes. In case of p21 protein, its level increased, similarly as in the human breast carcinoma cells, what may also be related to phosphorylation of the protein, its capture by the cytosol and prolonging its half-life as compared to the non-phosphorylated form. In case of the normal human 181A1 cells and mouse CRL 1636 cells, no significant alterations were noted in expression of the investigated genes in presence of the employed chitosan preparations.

10

UTILIZATION OF BIODEGRADABLE POLYMERS FOR PERIPHERAL NERVE RECONSTRUCTION

64%

Kardas I., Marcol W., Niekraszewicz A., Kucharska M., Ciechańska D., Wawro D., Lewin-Kowalik J., Właszczuk A.

Progress on Chemistry and Application of Chitin and its Derivatives

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2010

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vol. 15

159 - 168

EN

Peripheral nerve injuries are one of frequent cause of disability. Results of surgical procedures are not still completely satisfactory, though fast development of microsurgical technician improve their efficiency. One of the major problems in surgical treatment of the peripheral nerve injuries is ends coaptation in the case of large loss of nerve tissue. And so finding substitute implants not rejected by the organism for the recipient and providing with the success is necessary of regeneration. Recently, several synthetic nerve guide implants have been introduced and approved for clinical use to replace autologous transplants. This alternative therapy is based on pioneering studies with experimental nerve guides. This paper present a review of published studies involving biodegradable nerve guides.

11

HAEMOSTATIC, RESORBABLE DRESSING OF NATURAL POLYMERS - HEMOGUARD

52%

Kucharska M., Wiśniewska-Wrona M., Brzoza_Malczewska K., Struszczyk M. H., Cichecka M., Wilbik-Hałgas B., Rybak Z., Szymonowicz M., Paluch D., Guzińska K., Kaźmierczak D.

Progress on Chemistry and Application of Chitin and its Derivatives

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2015

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vol. 20

130-141

EN

Investigations are presented in the preparation of a first aid haemostatic dressing that would exhibit an adequate haemostatic capacity in injuries and surgical wounds, an antibacterial activity to prevent primary and secondary infections, and offer safety in use

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STUDIES ON THE USABLE PROPERTIES OF INNOVATIVE WOUND DRESSINGS

PRELIMINARY STUDIES ON THE USABLE PROPERTIES OF INNOVATIVE WOUND DRESSINGS

PREPARATION OF MICRO AND NANOSTRUCTURES OF CHITOSAN BY ULTRASONIC COALESCENCE OF W/O EMULSIONS

POLYMER BIOCOMPOSITES USED IN BEDSORES TREATMENT

THE ROLE OF OLIGOCHITOSANS IN AKT KINASE REGULATION

POTENTIAL USE OF CHITOSAN – BASED MATERIALE IN MEDICINE

INHIBITION OF EHRLICH ASCITES TUMOUR (EAT) CELLS PROLIFERATION THROUGH CHITOSAN-MEDIATED REGULATION OF ACTIVITY OF THE AKT PATHWAY

ADVANCED CELLULOSIC MATERIALS

THE ROLE OF CHITOSAN IN AKT KINASE REGULATION ACTIVITY

UTILIZATION OF BIODEGRADABLE POLYMERS FOR PERIPHERAL NERVE RECONSTRUCTION

HAEMOSTATIC, RESORBABLE DRESSING OF NATURAL POLYMERS - HEMOGUARD