Search results

Sort By:

Limit search:

Undescribed wheat gene for partial leaf rust and stripe rust resistance from Thatcher derivatives RL6058 and 90RN2491 carrying Lr34

100%

Agarwal S., Saini R. G.

Journal of Applied Genetics

2009

vol. 50

issue 3

199-204

Inheritance of partial leaf rust and stripe rust resistance of a Thatcher wheat 90RN2491, earlier reported to carry two doses of the gene pair Lr34-Yr18 and the reference line RL6058 (6*Thatcher/PI58548) for the Lr34-Yr18 gene pair was studied against predominant and highly virulent Indian races. Thatcher derivatives 90RN2491 and RL6058 were intercrossed as well as crossed with the leaf rust and stripe rust susceptible Indian cultivar WL711. The F1, F2 and F3 generations from these crosses were assessed for rust severity against leaf rust race 77-5 and stripe rust race 46S119. The F2 and F3 generations from the crosses of RL6058 and 90RN2491 with WL711, segregated 15 resistant : 1 susceptible (F2) and 7 homozygous resistant : 8 segregating : 1 homozygous susceptible (F3) ratios, respectively, both for leaf rust and stripe rust severity. Therefore, partial resistance against each of the leaf rust and stripe rust races in both RL6058 and 90RN2491 is ascribed to two independently inherited dominant genes. One of the two genes for leaf rust and stripe rust resistance in 90RN2491 and RL6058 is Lr34 and the linked gene Yr18, respectively. The second leaf rust resistance gene in both the Thatcher lines segregated independently of stripe rust resistance. Therefore, it is not Lr34 and it remains unidentified.

Genotyping of alpha-thalassemia in microcytic hypochromic anemia patients from North India

61%

Sankar V. H., Arya V., Tewari D., Gupta U. R., Pradhan M., Agarwal S.

Journal of Applied Genetics

2006

vol. 47

issue 4

391-395

Microcytic hypochromic anemia is a common condition in clinical practice and alpha-thalassemia has to be considered as a differential diagnosis. Molecular diagnosis of alph-thalassemia is possible by polymerase chain reaction. The aim of this study was to evaluate the frequency of alpha-gene numbers in subjects with microcytosis. In total, 276 subjects with microcytic hypochromic anemia [MCV<80fl; MCH<27pg] were studied. These include 125 with thalassemia trait, 48 with thalassemia major, 26 with sickle-cell thalassemia, 15 with E beta-thalassemia, 40 with iron-deficiency anemia, 8 with another hemolytic anemia, and 14 patients with no definite diagnosis. Genotyping for -alpha 3.7 deletion, alpha-4.2 deletion, Hb Constant Spring, and -triplications was done with polymerase chain reaction. The overall frequency of -alpha 3.7 deletion in 276 individuals is 12.7%. The calculated allele frequency for -thalassemia is 0.09. The subgroup analysis showed that co-inheritance of -deletion is more frequent with the sickle-cell mutation than in other groups. We were able to diagnose 1/3 of unexplained cases of microcytosis as -thalassemia carriers. The -gene mutation is quite common in the Indian subcontinent. Molecular genotyping of -thalassemia helps to diagnose unexplained microcytosis, and thus prevents unnecessary iron supplementation.

Refine search results

2 Journal of Applied Genetics

1 2009

1 2006

2 Agarwal S.

1 Arya V.

1 Gupta U. R.

1 Pradhan M.

1 Saini R. G.

1 Sankar V. H.

1 Tewari D.

Search results

Undescribed wheat gene for partial leaf rust and stripe rust resistance from Thatcher derivatives RL6058 and 90RN2491 carrying Lr34

Genotyping of alpha-thalassemia in microcytic hypochromic anemia patients from North India