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1

Pathways of aerobic petroleum oil hydrocarbons biodegradation

100%
Kwapisz E.
Biotechnologia
|
2006
|
issue 2
166-188
EN
The paper reviews the aspects of physiology and biochemistry of microbial biodegradation of the main components of petroleum oil: alkanes (larger than methane), alkenes, alkines, cyclic, polycyclic, aromatic and polyaromatic hydrocarbons. It focuses on aerobic degradation pathways. The review provides key information prepared on the basis of more than 30 years of research on microbial degradation of hydrocarbons. The areas discussed include new pathways of biodegradation of branched-chain alkanes, cyclic and polyaromatic hydrocarbons which have been discovered during the past few years. Special attention was paid to oxygenases ? enzymes initiating aerobic metabolism of hydrocarbons and phenomenon of co-oxidation which enables assimilation of most recalcitrant components of crude oil.
2

Microbial biotechnology - present state of research in Poland

63%
Galas E., Kwapisz E.
Biotechnologia
|
1995
|
issue 2
9-31
EN
The present state of science in microbial (classical) biotechnology in Poland is described.The main areas of research discussed in this paper include selection and industrial microorganisms improvement, optimization of biosynthesis processes, engineering aspects, techniques of immobilising cells and enzymes, processes of biotransformation and biodegradation, downstream processing and biotechnology in environmental protection.Special attention is paid to the problems of collaboration of scientific institutes with industry.The most important results in the above mentioned fields are also presented.
3
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Phytotests as tools for monitoring the bioremediation process of soil contaminated with diesel oil

51%
Wieczorek D., Kwapisz E., Marchut-Mikołajczyk O., Bielecki S.
BioTechnologia
|
2012
|
issue 4
4

Characterization of Candida methanosorbosa BP-6 strain capable of aniline degradation

51%
Mucha K., Kwapisz E., Kucharska U., Trzmiel T.
|
2007
|
issue 2
161-173
EN
The ability of some bacteria and filamentous fungi to degrade aniline and its derivatives has been reported in literature. This report is the first one concerning assimilation of aniline by Candida methanosorbosa BP-6 strain was isolated from wastewater dye factory by enrichement technique. Activity of this strain to degradation of aniline was compared with four bacterial strains: BD1, BZB1, BR 1, and PKN 7, isolated from the same environment and bacteria Bacillus subtilis IBT C-3 derived from the collection of Institute of Technical Biochemistry P?. Among the six tested strains capable to assimilate aniline, the most succesfull was Candida methanosorbosa BP-6 which quickly grows in the presence of high concentration of aniline (1-4%) and can degrade this substrate in the yield of 54-63%.
5

Screening methods of industrial strain mutants

51%
Galas E., Kwapisz E., Polak J.
Biotechnologia
|
1995
|
issue 3
104-119
EN
The screening methods used for improvement of industrial strains are presented.Two general categories of methods have been distinguished: random screening and rational screening.Major techniques employed in rational screening are based on the selection of auxotropic mutants, reverants of non-producers, strain resistant to some factors like toxic analogues of metabolism intermediates, induction and catabolite regression or end-product inhibition, mutants with modified cell wall permeability and microorganisms tolerating somenutrients and precursors of secondary metabolites.
6

Characterization of selected bacterial strains degrading hydrocarbons of petroleum oil

39%
Galas E., Kwapisz E., Tarabasz-Szymanska A., Krystynowicz A., Antczak T., Orynska
Biotechnologia
|
1997
|
issue 1
145-157
EN
The results of special tests which had been carried out with the following bacterail strains Bacillus sp. S2, Bacillus sp. BP, Pseudomonas sp. 141 and Micrococcus sp. R5.4 show that these strains are acive degraders of petroleum oil hydrocarbons, tolerate high concentrations of the subsrtate and have a high capability for degradation of hydrocarbons.The activity of dehydrogenases was related to the concentration of the substrate.The gas chromatography analysis of residual hydrocarbons in the medium after 9 days of cultivation proved that the main identified petroleum oil components had been metabolized with the yield 69 to 100%. Apart from the compounds originally present in crude oil no new by-products were detected in final medium.The accumulation of shorter chain length hydrocarbons and various new by-products occured in the case of another tested bacterial strain, designated 'B'.
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