Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 13

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Genetic modification of secondary metabolic pathways

100%
Wysokinska H.
Biotechnologia
|
2000
|
issue 4
48-61
EN
The examples given in this presentation show that the levels of secondary metabolites can be modified by genetic engineering methods. In these methods, the specific genes encoding enzymes involved in key steps of biosynthetic pathway can be transferred and expressed in cell lines, hairy root cultures or transgenic plants of the same or another species. Overexpression of the enzymes may lead to the accumulation of intermediates of pathways and increased synthesis of desirable products. Best results will be obtained when the overproduced enzyme activity is clearly the rate-limiting step in the pathway. Therefore, better knowledge of the limiting steps of the pathways is required. The successful of biosynthetic pathways regulation may also be achieved by expressing antisense RNA which is complementary to the mRNA encoding pathway enzyme or by genetic manipulation of degradative pathways. It should be mentioned that genetic engineering also plays a role in the development of novel plant-derived drugs. As secondary metabolites are important in the defense of plants against pathogens, such engineered plants may show an increase in resistance against pathogens.
2

Secondary metabolites from transformed root cultures

100%
Wysokinska H.
Biotechnologia
|
2000
|
issue 4
32-39
EN
Transformed roots in axenic culture would prove to be a good model for the study of the aspects of secondary metabolism. They are morphologically differentiated and have the advantage of high growth in the liquid standard media without growth regulators. Hairy root cultures can express root-specific pathways and have stable production of alkaloids, polyacetylenes, sesquiterpenes, naphthoquinones and other natural products. They can also convert xenobiotics into bioactive metabolites. Thus, new compounds not found in the parent plants could be obtained. Despite encouraging results, no commercial application of hairy root cultures for production of secondary metabolites have been developed, so far. A lot of further work is required to optimize bioreactor design for differentiated plant organ and to improve productivity of hairy roots.
3

Transformed root cultures of Arnica montana L. ? of secondary metabolites isolation

64%
Weremczuk-Jezyna I., Wysokinska H.
Biotechnologia
|
2004
|
issue 2
54-61
EN
The hairy roots of A. montana were achieved by the infection of sterile leaves with Agrobacterium rhizogenes (strain LBA 9402). The transformation was confirmed by opin and PCR analysis. In the investigation, the optimum contitions of Hairy roots growth were characterized. The best results were achieved in liquid Gamborg medium which included half-strenght macro- and microelements (1/2B5) and 50 g/l sucrose. Phytochemical analysis showed that hairy roots of A. montana produced arnifolin and chlorogenic acid.
4

Biotransformation by plant cell cultures. Part II. Process conditions

64%
Chmiel A., Wysokinska H.
Biotechnologia
|
1995
|
issue 1
131-147
EN
Biological and biochemical conditions of biotransformation processes ,applications of cell suspension, immobilised cells and enzyme preparations are discussed.The nature and form of biocatalysts determine the technical parameters of the process.Bioreactor systems for plant cell biotechnology are presented.
5

Biotransformation by plant cell cultures. Part I. Reactions

64%
Wysokinska H., Chmiel A.
Biotechnologia
|
1995
|
issue 1
114-130
EN
Plant enzymes are able to catalyse regio- and stereospecific reactions and can be applied to the production of pharmaceutically important compounds.This paper summarizes the results of such biotransformations in freely suspended cells.The factors affecting bioconversion capabilities of cells are also discussed.Special attention is paid to permeabilization and the problems of poorly water-soluble precursors.
6

Oxycoccus quadripetalus Gilib. in in vitro culture

64%
Floryanowicz-Czekalska K., Wysokinska H.
Biotechnologia
|
2004
|
issue 2
225-230
EN
Oxycoccus quadripetalus callus induction and study on its capacity to regenerate shoots and/or roots were established.Woody Plant (WP) medium with 2iP (10 M) and NAA (1 M) was most suitable for callus tissue induction. The highest frequency of shoot regeneration was observed for WP medium supplemented with TDZ (0,2 M) and NAA (1 M). Multiplication of O. quadripetalus shoots from seedling shoot tips was also realized. Ca 3 (7 cm long) shoots with 11 nodes per shoot were differentiated from one explant.
7

Micropropagation of Salvia officinalis L. by shoot tips

64%
Grzegorczyk I., Wysokinska H.
Biotechnologia
|
2004
|
issue 2
212-218
EN
In this study, optimum conditions for the micropropagation of Salvia officinalis by shoot tips were determined. Shoot tips from five-week-old shoots grown in in vitro culture were used as explants. The explants were incubated on MS agar medium supplemented with 0.57 ?mol/l IAA and various concentrations of cytokinins (BAP, zeatin, TDZ or kinetin). The best results were obtained when BAP at the concentration of 2 ?mol/l was used as cytokinin. Under these conditions after 5 weeks more than 90% shoot tips formed axillary buds or shoots and almost 3 shoots per one explant were obtained. An average length of shoots was 2.5 cm. For root induction the most suitable was ? MS agar medium without growth regulators.
8

Secondary metabolites production in cultures of transformed plant organs

64%
Wysokinska H., Chmiel A.
Biotechnologia
|
2006
|
issue 4
124-135
EN
Plant cell cultures in vitro produce secondary metabolites with varied effectiveness. Despite industrial application of only few cell suspension cultures, considerable progress in research on plant cell biotechnology has been made over the last few years. Transformed organ cultures, especially hairy roots, seem to be an interesting model for stable production of plant metabolites with high yield. In this paper, cultures of hairy roots of Salvia sclarea, S. officinalis, S. miltiorrhiza, S. przewalski, and Centaurium erythracea, as well as transformed shoots and plants of C. erythracea are presented. Also, production of secondary metabolites in these cultures is discussed.
9

Micropropagation of Arnica montana L. and sesquiterpene lactones in regenerated plants

64%
Weremczuk-Jezyna I., Wysokinska H.
Biotechnologia
|
2000
|
issue 4
118-122
EN
Plants of Arnica montana L. were micropropagated from shoot tips. Shoot proliferation was obtained on Murashige and Skoog (MS) medium containing auxin (IAA 0.5 M/l) and cytokinin (BAP, Z, TDZ). BAP (0.25 M/l) and Z (0.5 M/l) were most effective for proliferation and elongation of shoots. Under these conditions, about 6 shoots per explant were obtained. Shoot proliferation was also achieved in the presence of TDZ (0.05 M/l). However, when used in higher concentration (0.2-10 M/l), thidiazuron caused shoot vitrification. Rooting of shoots was induced an MS medium without growth regulators. The plantlets were transplanted into pots. Phytochemical analysis showed that 4-week-old plantlets produced sesquiterpene lactone.
10

Production of secondary metabolites in shoots of Salvia nemorosa L. cultured in vitro

64%
Kuzma L., Wysokinska H.
Biotechnologia
|
2003
|
issue 4
154-159
EN
The shoots of Salvia nemorosa were proliferated in vitro from shoot tips of 4-week-old seedlings on Murashige and Skoog (MS) agar (0.7%) medium containing indolilo-3-acetic acid (IAA) 0.5 mg/l and benzylaminopurine (BAP) 1 mg/l. Four sterols (campesterol, stigmasterol, beta-sitosterol, stigmastanol) and two triterpenes (ursolic acid, oleanolic acid) were detected by GC-MS method in the chloroform extract obtained from the dry in vitro cultured shoots of S. nemorosa. From the methanolic extract, rosmarinic acid both as isomer trans and cis was isolated and identified on the basis of 1H-NMR and HH-COSY spectral data. It is the first report on the detection of cis-rosmarinic acid in plants.
11

Ginsenosides contents in Panax quinquefolium cultures

51%
Kochan E., Gadomska G., Wysokinska H., Chmiel A.
Biotechnologia
|
2004
|
issue 1
236-243
EN
Contents of six main saponins (Rb1, Rb2, Rc i Rd ? protopanaxadiols, Rg1 i Re - protopanaxatriols) were investigated in callus and suspension cultures of Panax quinquefolium. HPLC method was used to indicate saponins quantatively. Qualitative composition and the amount of individual ginsenosides varied depending on culture age and light conditions. The younger calli, grown in dark, produced all indicated metabolites, and protopanaxatriols were dominant. In older callus cultures, only 3 of the saponins (Rg1, Re, Rb1) were present. The calli grown in light did not synthesize protopanaxadiols at all. Although suspension cultures contained all of the 6 above indicated metabolites, ginsenosides Re and Rd were predominant and reached the highest level, 2,7 mg/g d.w. and 1,2 mg/g d.w. respectively.
12

Composition of essential oil from Elsholtzia cristata Willd. shoots cultured in vitro

51%
Sienkiewicz-Chwist M., Kalemba D., Wysokinska H.
Biotechnologia
|
2004
|
issue 2
100-106
EN
In this work, we studied the composition of essential oil from Elsholtzia cristata shoot proliferating cultures grown on agar-solidified Murashige and Skoog medium supplemented with indole-3-acetic acid (0,1 mg/l) and 6-benzyloaminopurine (0,5 mg/l) for 4 weeks. The essential oils from shoots E. cristata and flowers of regenerated in vitro plants grown in the soil were also studied. The essential oil was obtained by hydrodistillation and analysed by GC-MS method. It was found that the essential oils from the shoot cultures, shoots and flowers of plants were at 0,1, 0,2 and 0,67% (v/w), respectively. The main component of essential oils isolated from these materials was estragol (80-90%) in contrast to intact plants and callus tissue with were reported in the literature to contain mainly elsholtzia ketones.
13

Hairy root culture of Centaurium erythraea Rafn.

45%
Piatczak E., Wielanek M., Krolicka A., Lojkowska E., Wysokinska H.
Biotechnologia
|
2004
|
issue 2
29-36
EN
In this study, the transformed root culture of Centaurium erythraea was established, as a result of infection of leaves culture with agropine strain of Agrobacterium rhizogenes (LBA 9402). Frequency of root formation depended on the kind of explants and the presence of acetosyringone in the bacteria medium. The transformation was confirmed according to the analysis of opines and RAPD-PCR. As the result of transformation 30 clones of hairy roots were obtained. These clones have grown in liquid Woody Plant (WPM) medium without growth regulators. The curve of growth indicates that fresh weight of roots increased almost 17 times , and dry weight increased 12 times during 55 days. Hairy roots of C. erythraea were able to produce secoiridoid glycosides (sweroside, gentiopicroside, swertiamarine).
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.