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A fast determination of yohimbine in pharmaceuticals by micellar electrokinetic chromatography

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Vítková K., Pechová M., Petr J., Znaleziona J., Ranc V., Maier V., Ševčík J.
Open Chemistry
|
2010
|
vol. 8
|
issue 2
273-277
EN
A method for the fast determination of yohimbine, a potent adrenoreceptor antagonist used for the treatment of sexual dysfunctions, is proposed in this article. MEKC under basic and acidic conditions (sodium borate, pH 9.5 and sodium phosphate, pH 2.5) with SDS was developed. The effect of the experimental parameters, e.g. pH, SDS concentration and injection time, on yohimbine migration was also studied. Both methods were validated in terms of linearity, limits of detection and quantification, accuracy, and precision using caffeine as an internal standard. The application for the determination of yohimbine in hand-made medicaments is also investigated in this study. [...]
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