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1

Methods of preservation of in vitro plant cell culture

100%
Marecik R., Kroliczak P.
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issue 1
105-116
EN
Tissue culture has been utilized for producing virus free plants for vegetative propagation and genetic engineering. In addition, altered cells lines showing higher productivity of secondary metabolities can be obtained using tissue culture. The wide use of tissue culture requires the development of new preservation techniques for in vitro culture material. One of this method is growth reduction achieved by modifying various parameters such as: temperature, culture medium, gasous environment. However, crypreservation (i. e. storage in liquid nitrogen. -196?C) is the only method available nowadays for long-term conservation. New cryopreservation techniques such as encapsulation-dehydratation, vitrification and desiccation helped expend the list of plant species that can tolerate low temperatures and are characterized by a normal rate of growth. Each step of the cryopreservation procces requires specific conditions.
2

Phytoremediation ? an alternative method for environmental cleanup

81%
Marecik R., Kroliczak P., Cyplik P.
Biotechnologia
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2006
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issue 3
88-97
EN
Phytoremediation is a new, promising, quickly developing technique for environmental clean-up. Some plants have the ability to accumulate contaminants by building them into the structure of their own cells, others can collect and metabolize toxins as a result of the natural process of adaptation to difficult life conditions in contaminated environment. The idea of introducing plant systems for environmental cleaning has many advantages, including significantly lower remediation costs compared to traditional methods, convenience of use, favourable effect on soil, and stimulation of the growth of microbial populations. Additionally, the technology is friendly to the environment and improves its aesthetic features.
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