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The effect of different incubation media of eggs of Archachatina marginata (Swainson, 1821) ovum in the University of Calabar, Nigeria

100%

Alozie J. C., Ogogo A., Achukee C. K., Bright J.

World News of Natural Sciences

2024

vol. 57

209-222

Hatchability of snail eggs in a cage is normally impaired as they are often unearthed by other snails looking for laying sites. This study evaluated the effects of different incubation media on egg hatchability of the giant African land snail (Archachatina marginata ovum) over a seven-week period. The experiment was conducted at the Department of Forestry and Wildlife Resources Management Teaching and Research Farm, University of Calabar, Calabar, Nigeria. One hundred eggs were collected from 2 boxes stocked with adult snails. The eggs were incubated in 4 media comprising river sand, top soil, sawdust, mixture of river sand, top soil and saw dust. Seventy-seven eggs were randomly assigned to each medium having six replicates of three eggs each incubated in a box measuring top diameter 11. 5 cm, bottom diameter 11 cm and height 9.5 cm. Data were collected on daily temperature (morning, and evening), length of incubation and % hatchability and subjected to one way analysis of variance in a completely randomized design. Duncan's Multiple Range Test was used to separate the means. Mean daily temperature ranged from 25.86-26.03 which was not significantly (P>0.05) different between incubation media. Incubation period ranged from 22 -30 days and was significantly (P<0.05) different between media. Eggs incubated in sawdust hatched earlier (25 days), followed by those in a mixture of river sand and sawdust, topsoil (26 days), river sand (27 days), and top soil gave the longest incubation period (28 days). Percentage Egg hatchability differed (P<0.05) significantly between incubation media. Sawdust gave 66.67 % hatchability, 50% was recorded in a mixture containing river sand and top soil and sawdust, river sand has 41.67% while topsoil gave the least hatchability of 25 %. It was thus recommended that saw dust could be adopted as the ideal incubating medium for eggs of Archachatina marginata Saturalis.

Effect of Tetracarpidium conophorum nut extracts on body and organ weights of monosodium glutamate obesity-induced in albino wistar rats

76%

Abraham E. J., Atangwho I. J., Achukee C. K., Udoubom I. A., Abang C., Ekon I. E., Nwankwor K. C.

World News of Natural Sciences

2024

vol. 52

1-11

This study aimed to assess the effect of Tetracarpidium conophorum nut extracts on body weight and organ/tissue weight in monosodium glutamate obesity- induced in Wistar rats. With the global obesity epidemic lacking an effective cure, this investigation holds significance. Twenty-five Wistar rats (15 males and 10 females) were utilized, housed in well-ventilated cages at a 3:2 female-to-male ratio Monosodium glutamate was induced in pups using intraperitoneal monosodium glutamate injections from postnatal days 2 through 10. Normal controls received saline, and all experimental animals were raised on standard rat chow until reaching a weight of ≥150g. The study encompassed five groups, each consisting of 7 animals. Groups IV, V, and VI were treated with Tetracarpidium conophorum nut extracts: ethanol whole extract (EWE), ethyl acetate extract (EAE), and ethanol residue (ER), respectively. Group III, the standard control was given 5.14g/kg of Orlistat reconstituted in normal saline. Experimental animals of groups I and II served as the normal and obese controls, respectively. After a six-week treatment period, the animals were euthanized for organ harvesting. Results indicated varying weight changes among treatment groups compared to controls. The EWE-treated group displayed a notable decrease in weight (-1.40 ± 8.42) compared to the obese control (8.29 ± 8.29). Similarly, EAE-treated animals exhibited weight reduction (-6.90 ± 12.29), as did the ER-treated group (-0.10 ± 12.22). Evidently, EWE treatment induced the most substantial weight loss. Tetracarpidium conophorum nut extracts demonstrated potential in alleviating obesity-related weight gain in the rat model.

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2 World News of Natural Sciences

2 2024

2 Achukee C. K.

1 Abang C.

1 Abraham E. J.

1 Alozie J. C.

1 Atangwho I. J.

1 Bright J.

1 Ekon I. E.

1 Nwankwor K. C.

1 Ogogo A.

1 Udoubom I. A.

Search results

The effect of different incubation media of eggs of Archachatina marginata (Swainson, 1821) ovum in the University of Calabar, Nigeria

Effect of Tetracarpidium conophorum nut extracts on body and organ weights of monosodium glutamate obesity-induced in albino wistar rats