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Defined serum-free culturing conditions for neural tissue engineering of human cord blood stem cells

100%
Ali H., Jurga M., Kurgonaite K., Forraz N., McGuckin C.
Acta Neurobiologiae Experimentalis
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2009
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vol. 69
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issue 1
12-23
EN
Taking tissue engineering applications into clinical trials requires the development of efficient and safe protocols incorporated with effective 3-dimentional cell culturing and differentiation systems in order to develop transplantable tissues that may offer a life-line for patients in the future. Cord blood, which is perhaps the most abundant world stem cell source, has shown previously practical and ethical advantages over other stem cells sources in many research and clinical applications including regenerative medicine. We previously developed a three-step protocol for isolation, expansion and sequential neuronal differentiation of cord blood pluripotent stem cells (characterized with our unique triple immunocytochemisty scheme for Oct-4, Sox-2 and Nanog) in defined serum-free culturing conditions. In this study we incorporated this protocol with 3-dimentional culturing systems which produced artificial neuronal tissues expressing Nestin, NF-200, TUJ1, PSD-95 and NeuN. We showed that cord blood pluripotent stem cells are a potential and promising candidate for future neural tissue engineering and regenerative medicine.
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Embryonic-like stem cells from umbilical cord blood and potential for neural modeling

86%
McGuckin C., Forraz N., Baradez M.-O., Basford C., Dickinson A. M., Navran S., Hartgerink J. D.
Acta Neurobiologiae Experimentalis
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2006
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vol. 66
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issue 4
321-329
EN
Stem cells offer the distinct prospect of changing the face of human medicine. However, although they have potential to form different tissues, are still in the early stages of development as therapeutic interventions. The three most used stem cell sources are umbilical cord blood, bone marrow and human embryos. Whilst, cord blood is now used to treat over 70 disorders, at the time of writing this manuscript, not a single disease has been overcome or ameliorated using human embryonic stem cells. Advancing stem cell medicine requires ethically sound and scientifically robust models to develop tomorrow's medicines. Media attention, however, distracts from this reality; it is important to remember that stem cells are a new visitor to the medical world and require more research. Here we describe the utility of human cord blood to develop neural models that are necessary to take stem cells to the next level ? into human therapies.
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