. Induced mutation rate of barley esterase loci has been estimated. Results suggested that about 3% of investigated M1 spikes had seeds which gave rise to M2 seedlings mutated in one of four esterase loci. M1 plants were obtained after chemical treatment of seeds from two spring barley cultivars Aramir and Bielik. The majority of mutants were reconfirmed in the M3 generation.
The usefulness of mutagenic treatment to enlarge isozymic variability of barley and the use of induced mutants for genetic analysis were evaluated.N-methyl-N-nitroso urea, sodium azide and gamma rays were employed as mutagenic agents>Electrophoretic assays of 3848 M2 seedlings obtained by chemical mutagenic treatment of the spring barley cultivars Dema, Aramir, Bielik and 3100 M2 seedlings obtained by physical mutagenic treatment of the cv. Dema revealed 70 isozymic mutants, which represent 30 separate mutants in 25 M1 plants.Most of mutations (27) were induced by chemical mutagen at polymorphic esterase loci.The occurence of induced mutants analysis of those loci in barley including mapping respective genes within chromosomes.
To saturate barley (Hordeum vulgare L.) genetic maps the linkage relationships of two isoenzyme loci Amp2 (aminopeptydase) and Aat2 (aspartate aminotransferase) with known genetic markers were investigated. Results of the genetic analysis support previous information on the localization of these loci on chromosome 1 and 6, respectively. The following recombination values were estimated: between locus Amp2 and T1-3b translocation break point 13.8 ? 2.1%, between locus Aat2 and translocation T6-7i, 17 ? 3.0% and between locus Aat2 and marker o 24.1 ? 3.0%.
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.