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1

Sialic acid containing lipopolysaccharides

100%
Gamian A.
Postępy Higieny i Medycyny Doświadczalnej
|
1996
|
vol. 50
|
issue 5
459-472
EN
Sialic acids which are important constituents of animal tissue glycoconjugates are also present in antigens of some bacterial strains. Capsular polysaccharides with sialic acid have been extensively studied whereas little is known on lipopolysaccharides which contain sialic acid. The paper presents review of the data concerning structure of bacterial endotoxic lipopolysaccharides. Methodological peculiarities were described. Specially emphasized were endotoxins of Escherichia coli O24, O56, O104, Salmonella toucra O48, Citrobacter freundii O37 and Hafnia alvei strain PCM2386.
2

Studies on glycyl componenet of lipopolysaccharides

64%
Mieszala M., Gamian A.
Postępy Higieny i Medycyny Doświadczalnej
|
1996
|
vol. 50
|
issue 5
525-528
EN
The aminoacyl analysis of endotoxic lipopolysaccharides (LPS) isolated from several bacteria revealed essential amounts of glycine, among the inherent LPS components. Glycine as a single amino acid was found only in a core part of LPS. The results indicate that amino acid is an integral constituent of core oligosaccharide in lipopolysaccharide.
3

Biochemical properties and clinical effects of the products formed during the glycation of proteins

64%
Staniszewska M., Gamian A.
Postępy Higieny i Medycyny Doświadczalnej
|
2003
|
vol. 57
|
issue 2
123-147
EN
Advanced glycation end-products (AGEs) are formed during non-enzymatic glycation - the process occurring in vitro and in the organism. The glycation products accumulate in tissues and interact with specific receptors, what induces various cellular responses. Some enzymes important in metabolism can be also glycated. The disturbances of homeostasis, related to the glycation products, are the reason for complications observed in diabetes and aging processes. There are presented in this paper: mechanism of the formation of AGEs, their cellular receptor (RAGE), as well as the effects of glycation in aging, diabetes and Alzheimer disease. Finally, there are described the compounds which could be useful as inhibitors of glycation in clinical practice.
4

Methods of measurement of the protein glycation products formed in human organism

64%
Staniszewska M., Gamian A.
Postępy Higieny i Medycyny Doświadczalnej
|
2003
|
vol. 57
|
issue 4
359-368
EN
The advanced glycation end-products (AGEs) are the compounds formed and accumulated in the organism. There is heterogenous group of the glycation products, which have great variety of structural and functional properties. The glycation end-products can be used as markers of the glycation process. Because of the great heterogeneity of the AGEs there is no specific test for their adequate measurement. In this paper there are presented the chromatographic, colorimetric, spectroscopic, mass-spectrometric and serological methods, which are used for determination of AGEs in biological samples. New procedures for the preparation of the model AGEs have been also described. Special attention has been paid to the immunochemical tests. For further assays the model AGE antigens have to be prepared.
5

Glycine as an inherent component of bacterial lipopolysaccharides

51%
Zielinska-Kuzniarz K., Mieszala M., Lipinski T., Gamian A.
Postępy Higieny i Medycyny Doświadczalnej
|
2003
|
vol. 57
|
issue 5
473-483
EN
The aminoacylation of bacterial polysaccharide antigens and the biological role of this phenomenon are poorly understood, although it might be relevant in the processes of the infection and immunity. Due to the lability of ester linked substituens on glycoconjugate antigens, usually such groups escaped detection during routine strustural investigation. Among the only few data available, these on the occurrence of glycine in endotoxic lipopolysaccharides of Gram-negative bacteria are well documented. This work summarises these data on glycine as an integral constituent of bacterial LPS and also on some other amino acid esters in teichoic acids and phosphatidylglycerol of Gram-positive bacteria.The possible functions are discucced of such noncarbohydrate ester linked substituets in bacterial antigens.
6

Fimbriae as a pathogenic factor of bacteria and a carrier for conjugate vaccines

51%
Staniszewska M., Witkowska D., Gamian A.
Postępy Higieny i Medycyny Doświadczalnej
|
2000
|
vol. 54
|
issue 6
727-747
EN
Fimbriae play important role as pathogenic factors in many bacteria by their adhesive properties. Adhesin is located at the tip fimbriae but also in other parts of fimbriae. Recent findings on structure of fimbriae genes and their expression for the biosynthesis and formulation of complete fimbriae have been described. Special attention was focused on the participation of fimbriae in the mechanism of pathogenesis and their specificity towards tissue receptors. Most recent studies have been performed on E. coli and Klebsiella and those data predominate in this work. Fimbriae can be used for the construction of vaccine as a proteinous carrier for haptenic carbohydrate epitopes. In conjugates fimbriae express distinct immunogenic, adjuvant and protective properties.
7

Advanced glycation end-products prepared in solution under high pressure contain epitopes distinct from those formed in the dry reaction at high temperature

51%
Staniszewska M., Jarosz J., Jon M., Gamian A.
Archivum Immunologiae et Therapiae Experimentalis
|
2005
|
vol. 53
|
issue 1
71-78
EN
Advanced glycation end-products play an important role in diseases related to diabetes and aging processes. Model compounds are synthesized in order to prepare the diagnostic and experimental tools for studying the mechanisms of pathogenesis. The objective of the present study was to accelerate glycation and upgrade its efficiency under high-pressure conditions.Aqueous solutions of proteins were kept with carbohydrates under a pressure of up to 850 MPa for several hours. Then the high-pressure glycation (HPG) products were fractionated on a Sephadex G-200 column and characterized with SDS-PAGE and MALDI-TOF mass spectrometry. The low-molecular-mass fraction of glycated proteins was separated from the two fractions containing high- and intermediate-molecular-mass cross-linked products of glycation. The products were then compared with those obtained with the high-temperature glycation (HTG) procedure carried out in dry conditions with a lyophilized mixture of substrates. The fractionated products were used to prepare rabbit sera. The immunoblotting experiments showed that the epitopes on the cross-linked glycation products formed in solution under high pressure differed from those originating in dry conditions at high temperature. Sera against the HPG products were specific to homologous material and did not interact with the fractions obtained by HTG. The antibodies against HTG products recognized HTG but not HPG products.
8

Routine clinical laboratory tests correspond to increased serum levels of 3-hydroxy fatty acids, markers of endotoxins, in cardiosurgery patients

45%
Krasnik L., Szponar B., Walczak M., Larsson L., Gamian A.
Archivum Immunologiae et Therapiae Experimentalis
|
2006
|
vol. 54
|
issue 1
55-60
EN
Introduction: Endotoxemia developing during cardiosurgery as elevated endotoxin concentrations in patient's serum may prevail over 24 h after operation. A major reason is thought to be increased gut permeability resulting in endotoxin and bacterial leakage. In this study we aimed to measure endotoxin levels on samples obtained during and after cardiovascular procedures and compare them with clinical observations and laboratory test results. Materials and Methods: 3-Hydroxy fatty acids (3-OH FAs) of 10?18 carbon chain length, chemical markers of endotoxin (lipopolysaccharide), were determined in patient sera by gas chromatography-mass spectrometry-based analysis. Results were compared with routine laboratory tests: blood morphology, urine, ALT, AST, bilirubin, kidney parameters, clotting parameters, and gasometry. Results: Of a total of 16 patients, 5 patients (group I) showed increased serum 3-OH FA levels and 11 patients (group II) did not show any change in 3-OH FA levels 24 h after operation. All group I patients revealed leukocytosis, two developed post-operative anemia. Significantly different changes were observed: the initial, pre-operative 3-OH FA levels were similar for both groups, while group I patients showed increased levels of all the studied 3-OH FAs during the operation (p0.05), and 3-OH C14 and 3-OH C16 remained elevated 24 h after the operation. Conclusions: Cardiosurgery may strongly promote gut endotoxin translocation to the blood in some patients. Prolonged leukocytosis, deep anemia, and increased liver dysfunction markers may indicate the need for observation of possible endotoxemia development. It is recommended to monitor the endotoxin level and/or endotoxemia markers in cardiosurgery patients.
9

Structures and serology of the O-specific polysaccharides of bacteria of the genus Citrobacter

45%
Knirel Y. A., Kocharova N. A., Bystrova O. V., Katzenellenbogen E., Gamian A.
Archivum Immunologiae et Therapiae Experimentalis
|
2002
|
vol. 50
|
issue 6
377-389
EN
The review presents the structures of the O-specific polysaccharides (O-antigens) of the lipopolysaccharides isolated from over 25 Citrobacter strains, which represent different species and serogroups. The correlation between O-antigen structure and immunospecificity as well as numerous cross-reactions between Citrobacter and other enterobacterial species are discussed.
10

Structural and serological charaqcterization of Hafnia alvei PCM 1185 and 1199 O-specific polysaccharides

45%
K?bler J., Mieszala M., Katzenellenbogen E., Gamian A., Romanowska E.
Postępy Higieny i Medycyny Doświadczalnej
|
1996
|
vol. 50
|
issue 5
515-517
EN
This work describes results of studies of O-specific oligosaccharide repeating units from lipopolysaccharides of two related Hafnia alvei serotypes. The linkage between O-antigen and the core region in PCM 1199 LPS has been also established. The O-acetyl residues present in the polysaccharides are involved in formation of epitopes.
11

The effect of packed red blood cell storage on arachidonic acid and advanced glycation end-product formation

39%
Lysenko L., Mierzchala M., Gamian A., Durek G., Kubler A., Kozlowski R., Sliwinski M.
Archivum Immunologiae et Therapiae Experimentalis
|
2006
|
vol. 54
|
issue 5
357-362
EN
Introduction: The transfusion of packed red blood cells (PRBCs) is a significant risk to blood recipients. Blood banking procedures permit the storage of PRBCs for up to 42 days. Storage of PRBCs can cause polymorphonuclear granulocytes (PMN) activation and the development of neutrophil-mediated transfusion-related acute lung injury. The aim of our study was to determine if PRBC storage has an influence on the formation of arachidonic acid (AA) and advanced glycation end products (AGEs). Materials and Methods: Twenty units of PRBCs were used to measure AA and AGE levels. The samples were taken on the 0th, 14th, 28th, and 42nd days of PRBC storage. The AA level was analyzed by gas-liquid chromatography-mass spectrometry and AGE level by an immunoenzymatic test. Results: During the first 14 days of PRBC storage, the AA level significantly increased and then slowly decreased. The AGE level increased continuously during the whole time of the study. In a model experiment, the AA glycoxidation product trans-2-nonenal (T2N) formed adducts in reaction with hemoglobin which were detectable with the test for AGE. Conclusions: It is highly probable that the observed increase in AGE level is related to the decrease in AA in PRBCs, which can be associated with the formation of toxic aldehydes, especially T2N and 4-hydroxynonenal (HNE), from AA. Glucose in the PRBCs (preservative solution) can contribute to AGE formation as well. The formation of AGEs, HNE, and T2N in PRBCs, their influence on PMNs in vitro, and confirmation of our assumption need further studies.
12
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Molecular Interaction between Bacterial Antigens and Macrophage Receptors Studied by Atomic Force Microscopy

39%
Targosz M., Czuba P., Biedroń R., Strus M., Gamian A., Marcinkiewicz J., Szymoński M.
Acta Physica Polonica A
|
2006
|
vol. 109
|
issue 3
421-426
EN
Atomic force spectroscopy was used to study interaction strengths between bacterial antigens and receptors on macrophages. This method allowed for a direct comparison of the interaction strengths in different systems studied at the level of single molecules.
13

Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose

33%
Katzenellenbogen E., Kocharova N. A., Korzeniowska-Kowal A., Gamian A., Bogulska M., Szostko B., Shashkov A. S., Knirel Y. A.
Archivum Immunologiae et Therapiae Experimentalis
|
2008
|
vol. 56
|
issue 5
347-352
EN
Introduction: Hafnia alvei is the only species of the genus Hafnia, which belongs to the family of Enterobacteriaceae. These Gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. Their lipopolysaccharides (LPSs) are important surface antigens which are responsible for the serological specificity and numerous cross-reactions with other enterobacterial genera. So far, 29 different O-polysaccharide (OPS, O-antigen) structures in Hafnia LPSs have been established and for some of them the molecular basis of the serological activity has been elucidated. Materials and Methods: OPS from H. alvei strain PCM 1219 was obtained by mild acid hydrolysis of the LPS followed by gel permeation chromatography of carbohydrate material on Sephadex G-50 column. The polysaccharide structure was determined using chemical methods as well as 13C NMR and 1H NMR spectroscopy. For serological studies, SDS-PAGE, immunoblotting, and passive hemagglutination tests were used. Results: The serological studies revealed a cross-reactivity of the LPSs of H. alvei PCM 1219 and a group of H. alvei strains with an O-antigen containing D-glucose 1-phosphate and [(R)-3-hydroxybutyramido]-D-glucose. The following structure of the OPS was established: 2)-alpha-d-Glcp-(1-PO4-6)-alpha-d-GlcpNAcyl-(14)-alpha-d-GalpNAc-(13)-beta-d-GalpNAc-(1- > 3---- OAc6<-1 alpga-d-Glcp where Acyl stands for (R)-3-hydroxybutyryl and the degree of O-acetylation is 70%. The structure of the core oligosaccharide was found to be typical of the genus Hafnia. Conclusions: Based on the OPS structure and serological results it was concluded that H. alvei strain PCM 1219 should be classified in the same serogroup as the H. alvei type strain ATCC 13337 and five other strains containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose in their O-antigens.
14
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Nasal carriage of various staphylococcal species in small ruminant lentivirus-infected asymptomatic goats

24%
Moroz A., Szaluś-Jordanow O., Czopowicz M., Brodzik K., Petroniec V., Augustynowicz-Kopeć E., Lutyńska A., Roszczynko M., Gołoś-Wójcicka A., Korzeniowska-Kowal A., Gamian A., Mickiewicz M., Frymus T., Petelicka H., Kaba J., Moroz A., Szaluś-Jordanow O., Czopowicz M., Brodzik K., Petroniec V., Augustynowicz-Kopeć E., Lutyńska A., Roszczynko M., Gołoś-Wójcicka A., Korzeniowska-Kowal A., Gamian A., Mickiewicz M., Frymus T., Petelicka H., Kaba J.
Polish Journal of Veterinary Sciences
|
2020
|
vol. 23
|
issue 2
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