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Problems Associated with Transferring of Engineering to Small Scale - Towards Theoretical Nanotechnology

100%
Kaczmarek J.
Acta Physica Polonica A
|
2016
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vol. 130
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issue 6
1295-1323
EN
This paper is seen as a review of attempts carried out by author in order to indicate problems related to transferring of engineering to smaller scales having in mind theoretical aspects. Two main problems are indicated. The first one is associated with question what kind of theory is appropriate for realization of design of devices and processes related to smaller scales including nanoscale. The second problem is related to determination of source of precision which is necessary for realization of the design. Solution of both problems needs elaboration of appropriate theories. One indicates that unified mechanics of materials based on collection of dynamical systems with dimensional reduction is appropriate for future realization of the design process. This is multi-scale description which unifies discrete models related to atomic scale, including molecular dynamics, and more averaged continuum descriptions. In order to determine corresponding source of precision for small scale processes the vacuum medium mechanics is introduced. Within vacuum medium mechanics we are able to determine attractor, expressed on the most fundamental elementary particle level, and responsible for self-organization manifested in molecular processes. Vacuum medium mechanics is considered as fundamental theory. Therefore we should estimate to what degree such a theory is stable with respect to large set of experimental results. In particular one investigates consequences of vacuum medium mechanics for theoretical biology. One accentuates that mechanisms of biological evolution and source of precision for nanotechnology expressed by means of vacuum medium mechanics are interrelated. Theoretical biology and nanotechnology should be described in consistent way in order to cooperate in a future. Both theories: unified mechanics of materials and vacuum medium mechanics are seen as basis for further development of theoretical nanotechnology.
2

A role of protein kinase C isoforms in tumorigenicity and apoptotic cells death

51%
Rybczynska M., Ksiazek K., Kaczmarek J.
Postępy Higieny i Medycyny Doświadczalnej
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2000
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vol. 54
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issue 6
777-796
EN
Protein kinase C comprises a family of at least 13 distinct serine/threonine kinase isoenzymes that have important action in transmembrane signal transduction pathways and have been reported to regulate cell proliferation, differentiation, cell-to-cell interaction, cytoskeletal functions, gene transcription, apoptosis and drug resistance. The results of investigations show differential redistribution isozymes in each organs and their specyfic activity in determined diseases.
3

Analyses of air samples for ascospores of Leptosphaeria maculans and L.biglobosa by light microscopy and molecular techniques

45%
Kaczmarek J., Jedryczka M., Fitt B. D. L., Lucas J. A., Latunde-Dada A. O.
Journal of Applied Genetics
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2009
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vol. 50
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issue 4
411-419
EN
Spores of many fungal pathogens are dispersed by wind. Detection of these airborne inocula is important in forecasting both the onset and the risk of epiphytotics. Species-specific primers targeted at the internal transcribed spacer (ITS) region of Leptosphaeria maculans and L. biglobosa ? the causal organisms of phoma stem canker and stem lesions of Brassica spp., including oilseed rape ? were used to detect DNA extracted from particles deposited on tapes obtained from a spore trap operated in Rarwino (northwest Poland) from September to November in 2004 and 2006. The quantities of DNA assessed by traditional end-point PCR and quantitative real-time PCR were compared to microscopic counts of airborne ascospores. Results of this study showed that fluctuations in timing of ascospore release corresponded to the dynamics of combined concentrations of DNA from L. maculans and L. biglobosa, with significant positive correlations between ascospore number and DNA yield. Thus the utilization of PCR-based molecular diagnostic techniques enabled the detection, identification, and accurate quantification of airborne inoculum at the species level. Moreover, real-time PCR was more sensitive than traditional PCR, especially in years with low ascospore numbers.
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