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Serological characterization of the O-specific polysaccharide of Providencia alcalifaciens O23

100%

Torzewska A., Kocharova A. N., Maszewska A., Knirel Y. A., Rozalski A.

Archivum Immunologiae et Therapiae Experimentalis

2004

vol. 52

issue 1

43-49

The genus Providencia belongs to the Enterobacteriaceae family and currently consists of five species: P.alcalifaciens, P.heimbachae, P.rettgerii, P.rustigianii and P.stuartii .The serological classification scheme of P.alcalifaciens, P.rustigianii and P.stuartii includes 63 O-serogroups and 30 H-serogroups. The O-antigenic specificity is defined by the structure of the O-antigen(O-specific polysaccharide ?OPS), a part of the lipopolysaccharide (LPS,endotoxin),one of the major components of the outer membrane of Gram-negative bacteria and an important virulence factor of these bacteria. Among the bacteria of the Enterobacteriaceae family, the genus Providencia is one of the least studied in respect to its LPS structure and antigenic specificity. Studies of the chemical structures and the serological specificity of the O-antigens aim at the elucidation of the molecular basis of the serological classification of Providenciasp. LPS and alkali-treated LPS of P.alcalifaciens O23 and serologically related P.rustigianii O14, P.mirabilis O13 and P.myxofaciens as well as O-antiserum against P.alcalifaciens O23 were used. Serological characterization of P.alcalifaciens O23 O-specific polysaccharide was done by use enzyme immunosorbent assay (EIA), passive hemolysis test (PHT)as well as by inhibition and sodium deoxycholate polyacrylamide gel electrophoresis (DOC-PAGE)of LPS and Western blot. The OPS of P.alcalifaciens,O23,contains an N-(D-glucuronoyl)-N-[(R)-1-carboxyethyl ]-L- lysine residue (GlcAAlaLys).The LPS of P.alcalifaciens,O23,and other LPSs containing AlaLys from Providencia and Proteus strains were tested with rabbit anti-P.alcalifiaciens O23 serum. The serological data showed that a GlcAAlaLys-associated epitope plays a role as an antigenic determinant in the P.alcalifaciens O23 OPS and revealed the particular importance of glucuronic acid and the carboxyethyl group for the binding of O23-specific antibodies.

Structures and serology of the O-antigens of Proteus strains classified into serogroup O17 and former serogroup O35

73%

Torzewska A., Grabowski S., Kondakova A. N., Toukach F. V., Senchenkova S. N., Shashkov A. S., Arbatsky N. P., Knirel Y. A., Rozalski A., Kaca W.

Archivum Immunologiae et Therapiae Experimentalis

2006

vol. 54

issue 4

277-282

Introduction: Bacteria of the genus Proteus are facultative pathogens which commonly cause urinary tract infections. Based on the serological specificity of the O-chain polysaccharide of the lipopolysaccharide (O-polysaccharide, O-antigen), strains of P. mirabilis and P. vulgaris have been classified into 60 serogroups. Studies on the chemical structure and serological specificity of the O-antigens aim at the elucidation of the molecular basis and improvement of the serological classification of these bacteria. Materials and Methods: The O-polysaccharide was prepared by acetic acid degradation of the lipopolysaccharide isolated from dried bacterial mass of each strain by hot phenol/water extraction. 1H- and 13C-NMR spectroscopy was used for structural studies. Serological studies were performed with rabbit O-antisera using enzyme immunosorbent assay, passive hemolysis test, and the inhibition of reactions in these assays as well DOC-PAGE and Western blot. Results: Four Proteus strains belonging to serogroups O17 and O35 were found to possess similar O-polysaccharide structures, in particular having the same carbohydrate backbone built up of tetrasaccharide repeating units. However, they differ in the presence or absence of additional substituents, such as phosphoethanolamine in P. mirabilis O17 and glucose in P. penneri O17, as well as in the pattern and degree of O-acetylation of various monosaccharide residues. Serological studies also showed close relationships between the O-antigens studied. Conclusions: Based on these data it is proposed to reclassify strain P. mirabilis PrK 61/57, formerly representing the O35 serogroup, into the serogroup O17 in the Kauffman-Perch classification system of Proteus.

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2 Archivum Immunologiae et Therapiae Experimentalis

1 2006

1 2004

2 Knirel Y. A.

2 Rozalski A.

2 Torzewska A.

1 Arbatsky N. P.

1 Grabowski S.

1 Kaca W.

1 Kocharova A. N.

1 Kondakova A. N.

1 Maszewska A.

1 Senchenkova S. N.

1 Shashkov A. S.

1 Toukach F. V.

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Serological characterization of the O-specific polysaccharide of Providencia alcalifaciens O23

Structures and serology of the O-antigens of Proteus strains classified into serogroup O17 and former serogroup O35