Search results

1

Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21.

100%

Nowacka J., Helszer Z., Walter Z., Płucienniczak A., Kałużewski B.

|

vol. 51

|

issue 4

995-1001

EN

Pre- and postnatal diagnosis of chromosomal aberrations is generally based on conventional cytogenetic analysis. In this paper, we have devised a quantitative polymerase chain reaction (Q-PCR) method to determine gene dose effects and applied it in cases of regular trisomy 21 as a model. The method is based on quantitative assessment of PCR products after using primers amplifying DNA fragments located in the pericentromeric, heterochromatic, euchromatic and telomeric regions of chromosome 21. A gene dose effect on the amount of PCR product in cases of trisomy 21 was confirmed. Moreover, a correlation between the amount of the PCR product of the examined sequences and their location in the chromosome was observed. The obtained results suggest that the Q-PCR technique can be applied in the diagnosis of aneuploidies.

2

Phosphorus fractions of crayfish haemolymph, serum and haemocyanin

71%

Gondko R., Helszer Z., Adamska M.

|

3

Haemocyanin of some Oniscidea

50%

Helszer Z., Gondko R.

Acta Biochimica Polonica

|

1991

|

vol. 38

|

issue 3

303-310

Refine search results

3 Acta Biochimica Polonica

1 2004

1 1991

1 1985

3 Helszer Z.

2 Gondko R.

1 Adamska M.

1 Kałużewski B.

1 Nowacka J.

1 Płucienniczak A.

1 Walter Z.

Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21.

Phosphorus fractions of crayfish haemolymph, serum and haemocyanin

Haemocyanin of some Oniscidea